ID   SACA3_BOVIN             Reviewed;         163 AA.
AC   A6QQ77;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Sperm acrosome membrane-associated protein 3;
DE   Flags: Precursor;
GN   Name=SPACA3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sperm surface membrane protein that may be involved in sperm-
CC       egg plasma membrane adhesion and fusion during fertilization. It could
CC       be a potential receptor for the egg oligosaccharide residue N-
CC       acetylglucosamine, which is present in the extracellular matrix over
CC       the egg plasma membrane. The processed form has no detectable
CC       bacteriolytic activity in vitro (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ASTL. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 22 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00680}.
CC   -!- CAUTION: Although it belongs to the glycosyl hydrolase 22 family, Ala-
CC       70 and Asn-87 are present instead of the conserved Glu and Asp which
CC       are active site residues. It is therefore expected that this protein
CC       lacks hydrolase activity. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC149686; AAI49687.1; -; mRNA.
DR   RefSeq; NP_001098477.1; NM_001105007.1.
DR   AlphaFoldDB; A6QQ77; -.
DR   SMR; A6QQ77; -.
DR   CORUM; A6QQ77; -.
DR   STRING; 9913.ENSBTAP00000000449; -.
DR   CAZy; GH22; Glycoside Hydrolase Family 22.
DR   PaxDb; A6QQ77; -.
DR   Ensembl; ENSBTAT00000000449; ENSBTAP00000000449; ENSBTAG00000000344.
DR   GeneID; 617955; -.
DR   KEGG; bta:617955; -.
DR   CTD; 124912; -.
DR   VEuPathDB; HostDB:ENSBTAG00000000344; -.
DR   VGNC; VGNC:55872; SPACA3.
DR   eggNOG; ENOG502S1F5; Eukaryota.
DR   GeneTree; ENSGT00940000161810; -.
DR   HOGENOM; CLU_111620_1_2_1; -.
DR   InParanoid; A6QQ77; -.
DR   OMA; MYCTDLL; -.
DR   OrthoDB; 1551203at2759; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000000344; Expressed in spermatid and 24 other tissues.
DR   GO; GO:0043159; C:acrosomal matrix; IEA:Ensembl.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:Ensembl.
DR   GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0036126; C:sperm flagellum; IBA:GO_Central.
DR   GO; GO:0003796; F:lysozyme activity; IEA:InterPro.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IBA:GO_Central.
DR   GO; GO:0035036; P:sperm-egg recognition; IEA:Ensembl.
DR   InterPro; IPR001916; Glyco_hydro_22.
DR   InterPro; IPR019799; Glyco_hydro_22_CS.
DR   InterPro; IPR000974; Glyco_hydro_22_lys.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR030058; LYZL3.
DR   PANTHER; PTHR11407; PTHR11407; 1.
DR   PANTHER; PTHR11407:SF25; PTHR11407:SF25; 1.
DR   Pfam; PF00062; Lys; 1.
DR   PRINTS; PR00137; LYSOZYME.
DR   PRINTS; PR00135; LYZLACT.
DR   SMART; SM00263; LYZ1; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS00128; GLYCOSYL_HYDROL_F22_1; 1.
DR   PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..163
FT                   /note="Sperm acrosome membrane-associated protein 3"
FT                   /id="PRO_0000375078"
FT   DOMAIN          36..163
FT                   /note="C-type lysozyme"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        41..161
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        65..149
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        99..114
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        110..128
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
SQ   SEQUENCE   163 AA;  18099 MW;  A74B5BD9236E4C1E CRC64;
     MEAGSWAPRR WPRPPGIVLL ALASVLSSLL SSGQARVYSR CELARVLQDF GLEGYRGYSL
     ADWICLAYFA SGFNTGAVDH EADGSTNSGI FQINSRKWCK NLNPNVPNLC QMYCSDLLNP
     NLKDTVICAM KITQEPQGLG SWEAWRHHCQ GKDLSDWVDG CEL