ID   SACA3_CANLF             Reviewed;         163 AA.
AC   B6VH75;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Sperm acrosome membrane-associated protein 3;
DE   AltName: Full=Sperm protein reactive with antisperm antibodies;
DE            Short=Sperm protein reactive with ASA;
DE   Flags: Precursor;
GN   Name=SPACA3; Synonyms=SPRASA;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Prendergast D., Woad K.J., Chamley L.W., Shelling A.N.;
RT   "Evolutionary conservation of SPRASA in various animal species.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sperm surface membrane protein that may be involved in sperm-
CC       egg plasma membrane adhesion and fusion during fertilization. It could
CC       be a potential receptor for the egg oligosaccharide residue N-
CC       acetylglucosamine, which is present in the extracellular matrix over
CC       the egg plasma membrane. The processed form has no detectable
CC       bacteriolytic activity in vitro (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ASTL. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 22 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00680}.
CC   -!- CAUTION: Although it belongs to the glycosyl hydrolase 22 family, Thr-
CC       70 and Asn-87 are present instead of the conserved Glu and Asp which
CC       are active site residues. It is therefore expected that this protein
CC       lacks hydrolase activity. {ECO:0000305}.
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DR   EMBL; FJ396442; ACJ06636.1; -; mRNA.
DR   RefSeq; NP_001184016.1; NM_001197087.1.
DR   RefSeq; XP_005624416.1; XM_005624359.1.
DR   RefSeq; XP_005624417.1; XM_005624360.2.
DR   AlphaFoldDB; B6VH75; -.
DR   SMR; B6VH75; -.
DR   STRING; 9612.ENSCAFP00000027123; -.
DR   CAZy; GH22; Glycoside Hydrolase Family 22.
DR   PaxDb; B6VH75; -.
DR   Ensembl; ENSCAFT00030030611; ENSCAFP00030026696; ENSCAFG00030016608.
DR   GeneID; 610702; -.
DR   KEGG; cfa:610702; -.
DR   CTD; 124912; -.
DR   eggNOG; ENOG502S1F5; Eukaryota.
DR   HOGENOM; CLU_111620_1_1_1; -.
DR   InParanoid; B6VH75; -.
DR   OMA; MYCTDLL; -.
DR   OrthoDB; 1551203at2759; -.
DR   TreeFam; TF324882; -.
DR   Proteomes; UP000002254; Unplaced.
DR   Bgee; ENSCAFG00000018355; Expressed in testis and 5 other tissues.
DR   GO; GO:0043159; C:acrosomal matrix; IEA:Ensembl.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:Ensembl.
DR   GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0036126; C:sperm flagellum; IBA:GO_Central.
DR   GO; GO:0003796; F:lysozyme activity; IEA:InterPro.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IBA:GO_Central.
DR   GO; GO:0035036; P:sperm-egg recognition; IEA:Ensembl.
DR   InterPro; IPR001916; Glyco_hydro_22.
DR   InterPro; IPR019799; Glyco_hydro_22_CS.
DR   InterPro; IPR000974; Glyco_hydro_22_lys.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR030058; LYZL3.
DR   PANTHER; PTHR11407; PTHR11407; 1.
DR   PANTHER; PTHR11407:SF25; PTHR11407:SF25; 1.
DR   Pfam; PF00062; Lys; 1.
DR   PRINTS; PR00137; LYSOZYME.
DR   PRINTS; PR00135; LYZLACT.
DR   SMART; SM00263; LYZ1; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS00128; GLYCOSYL_HYDROL_F22_1; 1.
DR   PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..163
FT                   /note="Sperm acrosome membrane-associated protein 3"
FT                   /id="PRO_0000375079"
FT   DOMAIN          36..163
FT                   /note="C-type lysozyme"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        41..161
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        65..149
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        99..114
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        110..128
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
SQ   SEQUENCE   163 AA;  18195 MW;  908A337C1830C9F9 CRC64;
     MEARSWAPRG HLCPPGSMLL AFASLLGCLL TSSQARVYSR CELAKVLQDF GMEGYRGYTL
     ADWVCLAYFT SGFNTAAVDH EADGSTNNGI FQINSRKWCK NLNTEVPNVC QMYCSDLLNP
     NLKDTVICAM KITQQPQGLA SWEAWRRHCQ GKDLTDWVDG CDL