SACA3_HUMAN
ID SACA3_HUMAN Reviewed; 215 AA.
AC Q8IXA5; Q7Z4Y5;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Sperm acrosome membrane-associated protein 3;
DE AltName: Full=Cancer/testis antigen 54;
DE Short=CT54;
DE AltName: Full=Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17;
DE AltName: Full=Lysozyme-like protein 3;
DE AltName: Full=Sperm lysozyme-like protein 1;
DE AltName: Full=Sperm protein reactive with antisperm antibodies;
DE Short=Sperm protein reactive with ASA;
DE Contains:
DE RecName: Full=Sperm acrosome membrane-associated protein 3, membrane form;
DE Contains:
DE RecName: Full=Sperm acrosome membrane-associated protein 3, processed form;
GN Name=SPACA3; Synonyms=LYC3, LYZL3, SLLP1, SPRASA; ORFNames=UNQ424/PRO862;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 88-109,
RP FUNCTION IN FERTILIZATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=12606493; DOI=10.1095/biolreprod.102.010108;
RA Mandal A., Klotz K.L., Shetty J., Jayes F.L., Wolkowicz M.J., Bolling L.C.,
RA Coonrod S.A., Black M.B., Diekman A.B., Haystead T.A.J., Flickinger C.J.,
RA Herr J.C.;
RT "SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like
RT protein of human spermatozoa.";
RL Biol. Reprod. 68:1525-1537(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=16014814; DOI=10.1095/biolreprod.105.041889;
RA Zhang K., Gao R., Zhang H., Cai X., Shen C., Wu C., Zhao S., Yu L.;
RT "Molecular cloning and characterization of three novel lysozyme-like genes,
RT predominantly expressed in the male reproductive system of humans,
RT belonging to the c-type lysozyme/alpha-lactalbumin family.";
RL Biol. Reprod. 73:1064-1071(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Sperm;
RX PubMed=14747161; DOI=10.1093/humrep/deh050;
RA Chiu W.W.C., Erikson E.K., Sole C.A., Shelling A.N., Chamley L.W.;
RT "SPRASA, a novel sperm protein involved in immune-mediated infertility.";
RL Hum. Reprod. 19:243-249(2004).
CC -!- FUNCTION: Sperm surface membrane protein that may be involved in sperm-
CC egg plasma membrane adhesion and fusion during fertilization. It could
CC be a potential receptor for the egg oligosaccharide residue N-
CC acetylglucosamine, which is present in the extracellular matrix over
CC the egg plasma membrane. The processed form has no detectable
CC bacteriolytic activity in vitro. {ECO:0000269|PubMed:12606493}.
CC -!- SUBUNIT: Interacts with ASTL. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasmic vesicle, secretory
CC vesicle, acrosome membrane {ECO:0000305}; Single-pass type II membrane
CC protein {ECO:0000305}. Note=Anterior acrosome in non-capacitated
CC spermatozoa and retained in the equatorial segment and in the luminal
CC face of both the inner and outer acrosomal membranes following
CC capacitation and the acrosome reaction. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IXA5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IXA5-2; Sequence=VSP_021329;
CC -!- TISSUE SPECIFICITY: The processed form is expressed in sperm (at
CC protein level). Expressed in testis, epididymis and placenta.
CC {ECO:0000269|PubMed:12606493, ECO:0000269|PubMed:16014814}.
CC -!- PTM: The processed form derives from the membrane form by proteolytic
CC processing.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 22 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00680}.
CC -!- CAUTION: Although it belongs to the glycosyl hydrolase 22 family, Thr-
CC 122 and Asn-139 are present instead of the conserved Glu and Asp which
CC are active site residues. It is therefore expected that this protein
CC lacks hydrolase activity. {ECO:0000305}.
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DR EMBL; AF216311; AAK01478.1; -; mRNA.
DR EMBL; AF099029; AAP97222.1; -; Genomic_DNA.
DR EMBL; AY358653; AAQ89016.1; -; mRNA.
DR EMBL; BC100886; AAI00887.1; -; mRNA.
DR EMBL; BC100887; AAI00888.1; -; mRNA.
DR CCDS; CCDS11275.1; -. [Q8IXA5-1]
DR RefSeq; NP_001304155.1; NM_001317226.1.
DR RefSeq; NP_776246.1; NM_173847.4. [Q8IXA5-1]
DR AlphaFoldDB; Q8IXA5; -.
DR SMR; Q8IXA5; -.
DR BioGRID; 125897; 29.
DR IntAct; Q8IXA5; 7.
DR STRING; 9606.ENSP00000269053; -.
DR CAZy; GH22; Glycoside Hydrolase Family 22.
DR GlyGen; Q8IXA5; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8IXA5; -.
DR PhosphoSitePlus; Q8IXA5; -.
DR BioMuta; SPACA3; -.
DR DMDM; 74723659; -.
DR MassIVE; Q8IXA5; -.
DR PaxDb; Q8IXA5; -.
DR PeptideAtlas; Q8IXA5; -.
DR PRIDE; Q8IXA5; -.
DR ProteomicsDB; 70978; -. [Q8IXA5-1]
DR ProteomicsDB; 70979; -. [Q8IXA5-2]
DR Antibodypedia; 15460; 71 antibodies from 20 providers.
DR DNASU; 124912; -.
DR Ensembl; ENST00000269053.8; ENSP00000269053.3; ENSG00000141316.13. [Q8IXA5-1]
DR Ensembl; ENST00000580599.5; ENSP00000463386.1; ENSG00000141316.13. [Q8IXA5-2]
DR GeneID; 124912; -.
DR KEGG; hsa:124912; -.
DR MANE-Select; ENST00000269053.8; ENSP00000269053.3; NM_173847.5; NP_776246.1.
DR UCSC; uc002hhs.2; human. [Q8IXA5-1]
DR CTD; 124912; -.
DR DisGeNET; 124912; -.
DR GeneCards; SPACA3; -.
DR HGNC; HGNC:16260; SPACA3.
DR HPA; ENSG00000141316; Tissue enriched (testis).
DR MIM; 612749; gene.
DR neXtProt; NX_Q8IXA5; -.
DR OpenTargets; ENSG00000141316; -.
DR PharmGKB; PA38101; -.
DR VEuPathDB; HostDB:ENSG00000141316; -.
DR eggNOG; ENOG502S1F5; Eukaryota.
DR GeneTree; ENSGT00940000161810; -.
DR HOGENOM; CLU_111620_1_1_1; -.
DR InParanoid; Q8IXA5; -.
DR OMA; MYCTDLL; -.
DR PhylomeDB; Q8IXA5; -.
DR TreeFam; TF324882; -.
DR PathwayCommons; Q8IXA5; -.
DR SignaLink; Q8IXA5; -.
DR BioGRID-ORCS; 124912; 13 hits in 1067 CRISPR screens.
DR ChiTaRS; SPACA3; human.
DR GeneWiki; SPACA3; -.
DR GenomeRNAi; 124912; -.
DR Pharos; Q8IXA5; Tbio.
DR PRO; PR:Q8IXA5; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q8IXA5; protein.
DR Bgee; ENSG00000141316; Expressed in left testis and 104 other tissues.
DR ExpressionAtlas; Q8IXA5; baseline and differential.
DR Genevisible; Q8IXA5; HS.
DR GO; GO:0043159; C:acrosomal matrix; IDA:UniProtKB.
DR GO; GO:0002080; C:acrosomal membrane; IDA:UniProtKB.
DR GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030141; C:secretory granule; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IBA:GO_Central.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IMP:UniProtKB.
DR GO; GO:0042117; P:monocyte activation; TAS:UniProtKB.
DR GO; GO:0043032; P:positive regulation of macrophage activation; TAS:UniProtKB.
DR GO; GO:0050766; P:positive regulation of phagocytosis; TAS:UniProtKB.
DR GO; GO:0035036; P:sperm-egg recognition; IEA:Ensembl.
DR InterPro; IPR001916; Glyco_hydro_22.
DR InterPro; IPR019799; Glyco_hydro_22_CS.
DR InterPro; IPR000974; Glyco_hydro_22_lys.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR030058; LYZL3.
DR PANTHER; PTHR11407; PTHR11407; 1.
DR PANTHER; PTHR11407:SF25; PTHR11407:SF25; 1.
DR Pfam; PF00062; Lys; 1.
DR PRINTS; PR00137; LYSOZYME.
DR PRINTS; PR00135; LYZLACT.
DR SMART; SM00263; LYZ1; 1.
DR SUPFAM; SSF53955; SSF53955; 1.
DR PROSITE; PS00128; GLYCOSYL_HYDROL_F22_1; 1.
DR PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Direct protein sequencing;
KW Disulfide bond; Membrane; Reference proteome; Secreted; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..215
FT /note="Sperm acrosome membrane-associated protein 3,
FT membrane form"
FT /id="PRO_0000256219"
FT CHAIN 88..215
FT /note="Sperm acrosome membrane-associated protein 3,
FT processed form"
FT /id="PRO_0000256220"
FT TOPO_DOM 1..63
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 88..215
FT /note="C-type lysozyme"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT SITE 87..88
FT /note="Cleavage; to produce processed form"
FT /evidence="ECO:0000305"
FT DISULFID 93..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT DISULFID 117..201
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT DISULFID 151..166
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT DISULFID 162..180
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT VAR_SEQ 1..69
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_021329"
FT VARIANT 80
FT /note="C -> Y (in dbSNP:rs16967845)"
FT /id="VAR_028885"
FT VARIANT 100
FT /note="H -> R (in dbSNP:rs28963)"
FT /id="VAR_028886"
FT VARIANT 128
FT /note="A -> T (in dbSNP:rs35420663)"
FT /id="VAR_050008"
SQ SEQUENCE 215 AA; 23431 MW; 39988565D592BCC8 CRC64;
MVSALRGAPL IRVHSSPVSS PSVSGPRRLV SCLSSQSSAL SQSGGGSTSA AGIEARSRAL
RRRWCPAGIM LLALVCLLSC LLPSSEAKLY GRCELARVLH DFGLDGYRGY SLADWVCLAY
FTSGFNAAAL DYEADGSTNN GIFQINSRRW CSNLTPNVPN VCRMYCSDLL NPNLKDTVIC
AMKITQEPQG LGYWEAWRHH CQGKDLTEWV DGCDF
