SACA4_HUMAN
ID SACA4_HUMAN Reviewed; 124 AA.
AC Q8TDM5;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Sperm acrosome membrane-associated protein 4 {ECO:0000305};
DE AltName: Full=Sperm acrosomal membrane-associated protein 14 {ECO:0000303|PubMed:12788941};
DE Flags: Precursor;
GN Name=SPACA4 {ECO:0000312|HGNC:HGNC:16441};
GN Synonyms=SAMP14 {ECO:0000303|PubMed:12788941};
GN ORFNames=UNQ3046/PRO9862 {ECO:0000303|PubMed:12975309};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 68-80, POSSIBLE FUNCTION IN
RP GAMETE INTERACTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR
RP LOCATION, GPI-ANCHOR AT ASN-97, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=12788941; DOI=10.1074/jbc.m301713200;
RA Shetty J., Wolkowicz M.J., Digilio L.C., Klotz K.L., Jayes F.L.,
RA Diekman A.B., Westbrook V.A., Farris E.M., Hao Z., Coonrod S.A.,
RA Flickinger C.J., Herr J.C.;
RT "SAMP14, a novel, acrosomal membrane-associated,
RT glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type
RT plasminogen activator receptor superfamily with a role in sperm-egg
RT interaction.";
RL J. Biol. Chem. 278:30506-30515(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Sperm surface membrane protein that may be involved in sperm-
CC egg plasma membrane adhesion and fusion during fertilization.
CC {ECO:0000269|PubMed:12788941}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12788941};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:12788941}. Cytoplasmic
CC vesicle, secretory vesicle, acrosome {ECO:0000269|PubMed:12788941}.
CC Note=Expressed in acrosomal matrix and outer and inner acrosomal
CC membranes. {ECO:0000269|PubMed:12788941}.
CC -!- TISSUE SPECIFICITY: Testis specific (PubMed:12788941). Expressed in
CC spermatozoa (PubMed:12788941). {ECO:0000269|PubMed:12788941}.
CC -!- SIMILARITY: Belongs to the SPACA4/bouncer family. {ECO:0000305}.
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DR EMBL; AF353721; AAL83950.1; -; mRNA.
DR EMBL; AY358387; AAQ88753.1; -; mRNA.
DR EMBL; BC047334; AAH47334.1; -; mRNA.
DR CCDS; CCDS12725.1; -.
DR RefSeq; NP_598005.1; NM_133498.2.
DR AlphaFoldDB; Q8TDM5; -.
DR SMR; Q8TDM5; -.
DR BioGRID; 128108; 24.
DR IntAct; Q8TDM5; 7.
DR STRING; 9606.ENSP00000312774; -.
DR BioMuta; SPACA4; -.
DR DMDM; 74730606; -.
DR MassIVE; Q8TDM5; -.
DR PaxDb; Q8TDM5; -.
DR PeptideAtlas; Q8TDM5; -.
DR PRIDE; Q8TDM5; -.
DR ProteomicsDB; 74301; -.
DR Antibodypedia; 49773; 29 antibodies from 12 providers.
DR DNASU; 171169; -.
DR Ensembl; ENST00000321762.3; ENSP00000312774.1; ENSG00000177202.3.
DR GeneID; 171169; -.
DR KEGG; hsa:171169; -.
DR MANE-Select; ENST00000321762.3; ENSP00000312774.1; NM_133498.3; NP_598005.1.
DR UCSC; uc002pjo.3; human.
DR CTD; 171169; -.
DR GeneCards; SPACA4; -.
DR HGNC; HGNC:16441; SPACA4.
DR HPA; ENSG00000177202; Tissue enriched (testis).
DR MIM; 609932; gene.
DR neXtProt; NX_Q8TDM5; -.
DR OpenTargets; ENSG00000177202; -.
DR PharmGKB; PA38141; -.
DR VEuPathDB; HostDB:ENSG00000177202; -.
DR eggNOG; ENOG502S5P1; Eukaryota.
DR GeneTree; ENSGT00510000049347; -.
DR HOGENOM; CLU_163755_0_0_1; -.
DR InParanoid; Q8TDM5; -.
DR OMA; TSCGREE; -.
DR OrthoDB; 1493078at2759; -.
DR PhylomeDB; Q8TDM5; -.
DR TreeFam; TF338445; -.
DR PathwayCommons; Q8TDM5; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; Q8TDM5; -.
DR BioGRID-ORCS; 171169; 18 hits in 1060 CRISPR screens.
DR GenomeRNAi; 171169; -.
DR Pharos; Q8TDM5; Tdark.
DR PRO; PR:Q8TDM5; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q8TDM5; protein.
DR Bgee; ENSG00000177202; Expressed in left testis and 67 other tissues.
DR ExpressionAtlas; Q8TDM5; baseline and differential.
DR Genevisible; Q8TDM5; HS.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0035036; P:sperm-egg recognition; IBA:GO_Central.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR046354; SPACA4/Bouncer.
DR PANTHER; PTHR47613; PTHR47613; 1.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell membrane; Cytoplasmic vesicle;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..97
FT /note="Sperm acrosome membrane-associated protein 4"
FT /id="PRO_0000252450"
FT PROPEP 98..124
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000252451"
FT DOMAIN 23..97
FT /note="UPAR/Ly6"
FT LIPID 97
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000305|PubMed:12788941"
FT DISULFID 23..47
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 26..34
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 41..65
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 71..90
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 91..96
FT /evidence="ECO:0000250|UniProtKB:P13987"
SQ SEQUENCE 124 AA; 13004 MW; 53407C5CCD8FC8BF CRC64;
MVLCWLLLLV MALPPGTTGV KDCVFCELTD SMQCPGTYMH CGDDEDCFTG HGVAPGTGPV
INKGCLRATS CGLEEPVSYR GVTYSLTTNC CTGRLCNRAP SSQTVGATTS LALGLGMLLP
PRLL
