SACA4_MOUSE
ID SACA4_MOUSE Reviewed; 127 AA.
AC Q80ZQ0; Q9DAK7;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Sperm acrosome membrane-associated protein 4 {ECO:0000305};
DE Flags: Precursor;
GN Name=Spaca4 {ECO:0000312|MGI:MGI:1916613};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Sperm surface membrane protein that may be involved in sperm-
CC egg plasma membrane adhesion and fusion during fertilization.
CC {ECO:0000250|UniProtKB:Q8TDM5}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8TDM5};
CC Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:Q8TDM5}. Cytoplasmic
CC vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q8TDM5}.
CC Note=Expressed in acrosomal matrix and outer and inner acrosomal
CC membranes. {ECO:0000250|UniProtKB:Q8TDM5}.
CC -!- SIMILARITY: Belongs to the SPACA4/bouncer family. {ECO:0000305}.
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DR EMBL; AK005760; BAB24224.1; -; mRNA.
DR EMBL; BC048608; AAH48608.1; -; mRNA.
DR CCDS; CCDS21262.1; -.
DR RefSeq; NP_081331.2; NM_027055.3.
DR AlphaFoldDB; Q80ZQ0; -.
DR STRING; 10090.ENSMUSP00000091991; -.
DR PaxDb; Q80ZQ0; -.
DR PRIDE; Q80ZQ0; -.
DR ProteomicsDB; 256687; -.
DR Antibodypedia; 49773; 29 antibodies from 12 providers.
DR Ensembl; ENSMUST00000094424; ENSMUSP00000091991; ENSMUSG00000070563.
DR GeneID; 69363; -.
DR KEGG; mmu:69363; -.
DR UCSC; uc009gwz.2; mouse.
DR CTD; 171169; -.
DR MGI; MGI:1916613; Spaca4.
DR VEuPathDB; HostDB:ENSMUSG00000070563; -.
DR eggNOG; ENOG502S5P1; Eukaryota.
DR GeneTree; ENSGT00510000049347; -.
DR HOGENOM; CLU_163755_0_0_1; -.
DR InParanoid; Q80ZQ0; -.
DR OMA; TSCGREE; -.
DR PhylomeDB; Q80ZQ0; -.
DR TreeFam; TF338445; -.
DR Reactome; R-MMU-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR BioGRID-ORCS; 69363; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q80ZQ0; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q80ZQ0; protein.
DR Bgee; ENSMUSG00000070563; Expressed in spermatid and 20 other tissues.
DR Genevisible; Q80ZQ0; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0035036; P:sperm-egg recognition; IBA:GO_Central.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR046354; SPACA4/Bouncer.
DR PANTHER; PTHR47613; PTHR47613; 1.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell membrane; Cytoplasmic vesicle; Disulfide bond;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..97
FT /note="Sperm acrosome membrane-associated protein 4"
FT /id="PRO_0000252452"
FT PROPEP 98..127
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000252453"
FT DOMAIN 23..97
FT /note="UPAR/Ly6"
FT LIPID 97
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000250|UniProtKB:Q8TDM5"
FT DISULFID 23..47
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 26..34
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 41..65
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 71..90
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT DISULFID 91..96
FT /evidence="ECO:0000250|UniProtKB:P13987"
FT CONFLICT 63
FT /note="K -> R (in Ref. 1; BAB24224)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 127 AA; 13380 MW; A4871B4A7F8C41B6 CRC64;
MVLGWPLLLV LVLCPGVTGI KDCVFCELTD SARCPGTHMR CGDDEDCFTG HGVAQGVGPI
INKGCVHSTS CGREEPISYM GLTYSLTTTC CSGHLCNKGT GLSTGATSLS LGLQLLLGLL
LLLQYWL
