SACA9_MOUSE
ID SACA9_MOUSE Reviewed; 168 AA.
AC Q7TPM5; Q3TTJ0; Q3U293; Q9D9W4;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Sperm acrosome-associated protein 9;
DE AltName: Full=Acrosome and sperm tail protein {ECO:0000303|PubMed:24256100, ECO:0000303|Ref.4};
DE Short=MAST {ECO:0000303|PubMed:24256100, ECO:0000303|Ref.4};
GN Name=Spaca9 {ECO:0000312|MGI:MGI:1917237};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RC STRAIN=RIII; TISSUE=Sperm;
RA Bhattacharya R., Dhople V.M., Jesudasan R.A.;
RT "Novel miRNA cluster generated by extensive alternate splicing of a
RT multicopy non-coding RNA from mouse Y-heterochromatin.";
RL Submitted (JUN-2009) to UniProtKB.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND INTERACTION WITH CABP1 AND CALR.
RX PubMed=24256100; DOI=10.1186/1471-2121-14-50;
RA Bhattacharya R., Devi M.S., Dhople V.M., Jesudasan R.A.;
RT "A mouse protein that localizes to acrosome and sperm tail is regulated by
RT Y-chromosome.";
RL BMC Cell Biol. 14:50-50(2013).
RN [7]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP INDUCTION.
RX PubMed=27914912; DOI=10.1016/j.ydbio.2016.11.019;
RA Stauber M., Weidemann M., Dittrich-Breiholz O., Lobschat K., Alten L.,
RA Mai M., Beckers A., Kracht M., Gossler A.;
RT "Identification of FOXJ1 effectors during ciliogenesis in the foetal
RT respiratory epithelium and embryonic left-right organiser of the mouse.";
RL Dev. Biol. 423:170-188(2017).
CC -!- SUBUNIT: Interacts with CABP1 AND CALR (PubMed:24256100). Interacts
CC with INCA1 (By similarity). {ECO:0000250|UniProtKB:Q4V8P4,
CC ECO:0000269|PubMed:24256100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24256100}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:24256100}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000269|PubMed:27914912}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:27914912}. Nucleus {ECO:0000250|UniProtKB:Q4V8P4}.
CC Note=In caudal sperms localizes onto sperm head. Is also present on
CC midpiece and principle piece of sperm tails. Acrosome and sperm tail
CC localization is regulated by Y-chromosome.
CC {ECO:0000269|PubMed:24256100}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TPM5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TPM5-2; Sequence=VSP_046487, VSP_046488;
CC -!- TISSUE SPECIFICITY: Expressed in sperm (at protein level) (Ref.4).
CC Expressed from almost all the cell types of testis, with abundant
CC expression in round and elongated spermatids (at protein level)
CC (PubMed:24256100). Predominantly expressed in tissues containing motile
CC cilia (PubMed:27914912). {ECO:0000269|PubMed:24256100,
CC ECO:0000269|PubMed:27914912, ECO:0000269|Ref.4}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing fetal lung epithelium
CC (at protein level). {ECO:0000269|PubMed:27914912}.
CC -!- INDUCTION: Expression is activated by FOXJ1 and NOTO.
CC {ECO:0000269|PubMed:27914912}.
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DR EMBL; AK006393; BAB24565.1; -; mRNA.
DR EMBL; AK155408; BAE33249.1; -; mRNA.
DR EMBL; AK161340; BAE36335.1; -; mRNA.
DR EMBL; CH466542; EDL08395.1; -; Genomic_DNA.
DR EMBL; BC055108; AAH55108.1; -; mRNA.
DR CCDS; CCDS15845.1; -. [Q7TPM5-1]
DR RefSeq; NP_081559.1; NM_027283.1. [Q7TPM5-1]
DR AlphaFoldDB; Q7TPM5; -.
DR SMR; Q7TPM5; -.
DR STRING; 10090.ENSMUSP00000121846; -.
DR iPTMnet; Q7TPM5; -.
DR PhosphoSitePlus; Q7TPM5; -.
DR MaxQB; Q7TPM5; -.
DR PaxDb; Q7TPM5; -.
DR PeptideAtlas; Q7TPM5; -.
DR PRIDE; Q7TPM5; -.
DR ProteomicsDB; 256584; -. [Q7TPM5-1]
DR ProteomicsDB; 256585; -. [Q7TPM5-2]
DR Antibodypedia; 18219; 59 antibodies from 15 providers.
DR DNASU; 69987; -.
DR Ensembl; ENSMUST00000102877; ENSMUSP00000099941; ENSMUSG00000026809. [Q7TPM5-1]
DR Ensembl; ENSMUST00000124840; ENSMUSP00000121846; ENSMUSG00000026809. [Q7TPM5-1]
DR GeneID; 69987; -.
DR KEGG; mmu:69987; -.
DR UCSC; uc008iza.1; mouse. [Q7TPM5-1]
DR UCSC; uc008izc.1; mouse. [Q7TPM5-2]
DR CTD; 11092; -.
DR MGI; MGI:1917237; Spaca9.
DR VEuPathDB; HostDB:ENSMUSG00000026809; -.
DR eggNOG; ENOG502QQV9; Eukaryota.
DR GeneTree; ENSGT00390000007746; -.
DR HOGENOM; CLU_100443_0_0_1; -.
DR InParanoid; Q7TPM5; -.
DR OMA; EHHCYNS; -.
DR OrthoDB; 1252395at2759; -.
DR PhylomeDB; Q7TPM5; -.
DR TreeFam; TF328897; -.
DR BioGRID-ORCS; 69987; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Spaca9; mouse.
DR PRO; PR:Q7TPM5; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q7TPM5; protein.
DR Bgee; ENSMUSG00000026809; Expressed in seminiferous tubule of testis and 86 other tissues.
DR Genevisible; Q7TPM5; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0097546; C:ciliary base; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005881; C:cytoplasmic microtubule; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IDA:MGI.
DR GO; GO:0048306; F:calcium-dependent protein binding; IPI:UniProtKB.
DR InterPro; IPR027818; SPACA9.
DR PANTHER; PTHR32455; PTHR32455; 1.
DR Pfam; PF15120; SPACA9; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm;
KW Cytoplasmic vesicle; Cytoskeleton; Flagellum; Nucleus; Reference proteome.
FT CHAIN 1..168
FT /note="Sperm acrosome-associated protein 9"
FT /id="PRO_0000089674"
FT SITE 117
FT /note="Essential for interaction with INCA1"
FT /evidence="ECO:0000250|UniProtKB:Q4V8P4"
FT SITE 119
FT /note="Essential for interaction with INCA1"
FT /evidence="ECO:0000250|UniProtKB:Q4V8P4"
FT VAR_SEQ 117..133
FT /note="YPHEVVNHLSCDEARNH -> GRHSFCTVASSGGDIQE (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_046487"
FT VAR_SEQ 134..168
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_046488"
FT CONFLICT 115
FT /note="A -> D (in Ref. 1; BAB24565)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 168 AA; 19482 MW; 650C8FE0B6BA325E CRC64;
MNEVKESLRS IEQKYKLFQQ QQFTFIAALE HCRENAHDKI RPISSIEQVQ SYMEHYCNNS
THRRILIMFM DICSELSKLC QHFEALHSGT PVTNSLLEKC KTLVSQSNDL SSLRAKYPHE
VVNHLSCDEA RNHYGGVVSL IPIVLDFMKE WIAHSEKLPR KVLQHGTT
