SACA9_RAT
ID SACA9_RAT Reviewed; 168 AA.
AC Q4V8P4; Q7TSB9;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Sperm acrosome-associated protein 9;
GN Name=Spaca9; Synonyms=Rsb66 {ECO:0000303|PubMed:18756329};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH INCA1,
RP TISSUE SPECIFICITY, MUTAGENESIS OF TYR-117 AND HIS-119, AND SITES TYR-117
RP AND HIS-119.
RX PubMed=18756329; DOI=10.1139/o08-072;
RA Chen X., Hu T., Liang G., Yang M., Zong S., Miao S., Koide S.S., Wang L.;
RT "A novel testis protein, RSB-66, interacting with INCA1 (inhibitor of Cdk
RT interacting with cyclin A1).";
RL Biochem. Cell Biol. 86:345-351(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with CABP1 AND CALR (By similarity). Interacts with
CC INCA1 (PubMed:18756329). {ECO:0000250|UniProtKB:Q7TPM5,
CC ECO:0000269|PubMed:18756329}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18756329}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000250|UniProtKB:Q7TPM5}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000250|UniProtKB:Q7TPM5}. Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q7TPM5}. Nucleus {ECO:0000269|PubMed:18756329}.
CC Note=In caudal sperms localizes onto sperm head. Is also present on
CC midpiece and principle piece of sperm tails. Acrosome and sperm tail
CC localization is regulated by Y-chromosome.
CC {ECO:0000250|UniProtKB:Q7TPM5}.
CC -!- TISSUE SPECIFICITY: Testis-specific. Expressed in round spermatids.
CC {ECO:0000269|PubMed:18756329}.
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DR EMBL; AY121839; AAN07900.1; -; mRNA.
DR EMBL; AC129847; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH474001; EDL93400.1; -; Genomic_DNA.
DR EMBL; BC097268; AAH97268.1; -; mRNA.
DR RefSeq; NP_859045.2; NM_181694.2.
DR AlphaFoldDB; Q4V8P4; -.
DR SMR; Q4V8P4; -.
DR STRING; 10116.ENSRNOP00000017004; -.
DR PaxDb; Q4V8P4; -.
DR GeneID; 296610; -.
DR KEGG; rno:296610; -.
DR CTD; 11092; -.
DR RGD; 727909; Spaca9.
DR VEuPathDB; HostDB:ENSRNOG00000012697; -.
DR eggNOG; ENOG502QQV9; Eukaryota.
DR HOGENOM; CLU_100443_0_0_1; -.
DR InParanoid; Q4V8P4; -.
DR OMA; EHHCYNS; -.
DR OrthoDB; 1252395at2759; -.
DR PhylomeDB; Q4V8P4; -.
DR TreeFam; TF328897; -.
DR PRO; PR:Q4V8P4; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Proteomes; UP000234681; Chromosome 3.
DR Bgee; ENSRNOG00000012697; Expressed in testis and 5 other tissues.
DR GO; GO:0001669; C:acrosomal vesicle; ISO:RGD.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0097546; C:ciliary base; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005881; C:cytoplasmic microtubule; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; ISO:RGD.
DR GO; GO:0048306; F:calcium-dependent protein binding; ISO:RGD.
DR InterPro; IPR027818; SPACA9.
DR PANTHER; PTHR32455; PTHR32455; 1.
DR Pfam; PF15120; SPACA9; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton;
KW Flagellum; Nucleus; Reference proteome.
FT CHAIN 1..168
FT /note="Sperm acrosome-associated protein 9"
FT /id="PRO_0000445518"
FT SITE 117
FT /note="Essential for interaction with INCA1"
FT /evidence="ECO:0000269|PubMed:18756329"
FT SITE 119
FT /note="Essential for interaction with INCA1"
FT /evidence="ECO:0000269|PubMed:18756329"
FT MUTAGEN 117
FT /note="Y->A: Significantly reduced interaction with INCA1;
FT when associated with A-119."
FT /evidence="ECO:0000269|PubMed:18756329"
FT MUTAGEN 119
FT /note="H->A: Significantly reduced interaction with INCA1;
FT when associated with A-117."
FT /evidence="ECO:0000269|PubMed:18756329"
FT CONFLICT 48
FT /note="Q -> L (in Ref. 1; AAN07900)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="N -> I (in Ref. 1; AAN07900)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 168 AA; 19465 MW; DEEDAE5AA363B277 CRC64;
MNEVKESLRS IEQKYKLFQQ QQFTFIAALE HCRENAHDKI RPISSIEQVQ SYMEHYCNNS
TDRRILLMFM DICSELNKLC QHFEALHSGT PVTNSLLEKC KTLVSQSNDL SILRAKYPHD
VVNHLSCDEA RNHYGGVVSL IPIVLDLMKE WIAHSEKLPR KVLQHGTT
