ID   SACB_ERWAM              Reviewed;         415 AA.
AC   Q46654;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Levansucrase;
DE            EC=2.4.1.10;
DE   AltName: Full=Beta-D-fructofuranosyl transferase;
DE   AltName: Full=Sucrose 6-fructosyl transferase;
GN   Name=lsc;
OS   Erwinia amylovora (Fire blight bacteria).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=552;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EA7/74;
RX   AGRICOLA=IND20389464; DOI=10.1006/pmpp.1993.1029;
RA   Geier G., Geider K.K.;
RT   "Characterization and influence on virulence of the levansucrase gene from
RT   the fireblight pathogen Erwinia amylovora.";
RL   Physiol. Mol. Plant Pathol. 42:387-404(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside +
CC         sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-
CC         glucopyranoside + D-glucose; Xref=Rhea:RHEA:13653, Rhea:RHEA-
CC         COMP:13093, Rhea:RHEA-COMP:13094, ChEBI:CHEBI:4167,
CC         ChEBI:CHEBI:17992, ChEBI:CHEBI:134464; EC=2.4.1.10;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR   EMBL; X75079; CAA52972.1; -; Genomic_DNA.
DR   PIR; S39195; S39195.
DR   AlphaFoldDB; Q46654; -.
DR   SMR; Q46654; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   BRENDA; 2.4.1.10; 2136.
DR   PHI-base; PHI:2472; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050053; F:levansucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Secreted; Transferase.
FT   CHAIN           1..415
FT                   /note="Levansucrase"
FT                   /id="PRO_0000057717"
FT   ACT_SITE        46
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        287
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            203
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
SQ   SEQUENCE   415 AA;  46408 MW;  4FF564F6E0607FEB CRC64;
     MSDYNYKPTL WTRADALKVH EDDPTTTQPV IDIAFPVMSE EVFIWDTMPL RDFDGEIISV
     NGWCIIFTLT ADRNTDNPQF QDENGNYDIT RDWEDRHGRA RICYWYSRTG KDWIFGGRVM
     AEGVAPTTRE WAGTPILLND RGDIDLYYTC VTPGATIAKV RGKIVTSDQS VSLEGFQQVT
     SLFSADGTIY QTEEQNAFWN FRDPSPFIDR NDGKLYMLFE GNVAGPRGSH EITQAEMGNV
     PPGYEDVGGA KYQAGCVGLA VAKDLSGSEW QILPPLITAV GVNDQTERPH FVFQDGKYYL
     FTISHKYTFA DNLTGPDGVY GFVSDKLTGP YTPMNSSGLV LGNPSSQPFQ TYSHYVMPNG
     LVTSFIDSVP WKGKDYRIGG TEAPTVKILL KGDRSFIVDS FDYGYIPAMK DITLK