ID   SACB_PSESG              Reviewed;         415 AA.
AC   O52408;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Levansucrase;
DE            EC=2.4.1.10;
DE   AltName: Full=Beta-D-fructofuranosyl transferase;
DE   AltName: Full=Sucrose 6-fructosyl transferase;
GN   Name=lsc;
OS   Pseudomonas savastanoi pv. glycinea (Pseudomonas syringae pv. glycinea).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=318;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PG4180;
RX   PubMed=9726857; DOI=10.1128/aem.64.9.3180-3187.1998;
RA   Hettwer U., Jaeckel F.R., Boch J., Meyer M., Rudolph K., Ullrich M.S.;
RT   "Cloning, nucleotide sequence, and expression in Escherichia coli of
RT   levansucrase genes from the plant pathogens Pseudomonas syringae pv.
RT   glycinea and P. syringae pv. phaseolicola.";
RL   Appl. Environ. Microbiol. 64:3180-3187(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside +
CC         sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-
CC         glucopyranoside + D-glucose; Xref=Rhea:RHEA:13653, Rhea:RHEA-
CC         COMP:13093, Rhea:RHEA-COMP:13094, ChEBI:CHEBI:4167,
CC         ChEBI:CHEBI:17992, ChEBI:CHEBI:134464; EC=2.4.1.10;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR   EMBL; AF037443; AAC36056.1; -; Genomic_DNA.
DR   RefSeq; WP_004664779.1; NZ_RBVH01000735.1.
DR   AlphaFoldDB; O52408; -.
DR   SMR; O52408; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   PATRIC; fig|318.3.peg.3339; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050053; F:levansucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Secreted; Transferase.
FT   CHAIN           1..415
FT                   /note="Levansucrase"
FT                   /id="PRO_0000057718"
FT   ACT_SITE        46
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        287
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            203
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
SQ   SEQUENCE   415 AA;  45844 MW;  839B686AC80610CF CRC64;
     MSNINYAPTI WSRADALKVN ENDPTTTQPL VSPDFPVMSD TVFIWDTMPL RELDGTVVSV
     NGWSVIVTLT ADRHPDDPQY VGANGRYDIK RDWEDRHGRA RMCYWYSRTG KDWIFGGRVM
     AEGVSPTTRE WAGTPVLLND KGDIDLYYTC VTPGAAIAKV RGRIVTSDKG VELKDFTEVK
     TLFEADGKYY QTEAQNSTWN FRDPSPFIDP NDGKLYMVFE GNVAGERGTH TVGAAELGPV
     PPGHEETGGA RFQVGCIGLA VAKDLSGDEW EILPPLVTAV GVNDQTERPH YVFQDGKYYL
     FTISHKFTYA DGVTGPDGVY GFVGEHLFGP YRPMNASGLV LGNPPAQPFQ TYSHCVMPNG
     LVTSFIDSVP TSGEDYRIGG TEAPTVRILL EGDRSFVQEV YDYGYIPAMK NVVLS