ID   SACB_PSESH              Reviewed;         431 AA.
AC   O68609;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Levansucrase;
DE            EC=2.4.1.10;
DE   AltName: Full=Beta-D-fructofuranosyl transferase;
DE   AltName: Full=Sucrose 6-fructosyl transferase;
GN   Name=lsc;
OS   Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv.
OS   phaseolicola).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=319;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LMG 5473 / NCPPB 1321;
RX   PubMed=9726857; DOI=10.1128/aem.64.9.3180-3187.1998;
RA   Hettwer U., Jaeckel F.R., Boch J., Meyer M., Rudolph K., Ullrich M.S.;
RT   "Cloning, nucleotide sequence, and expression in Escherichia coli of
RT   levansucrase genes from the plant pathogens Pseudomonas syringae pv.
RT   glycinea and P. syringae pv. phaseolicola.";
RL   Appl. Environ. Microbiol. 64:3180-3187(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside +
CC         sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-
CC         glucopyranoside + D-glucose; Xref=Rhea:RHEA:13653, Rhea:RHEA-
CC         COMP:13093, Rhea:RHEA-COMP:13094, ChEBI:CHEBI:4167,
CC         ChEBI:CHEBI:17992, ChEBI:CHEBI:134464; EC=2.4.1.10;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR   EMBL; AF052289; AAC36063.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68609; -.
DR   SMR; O68609; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050053; F:levansucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Secreted; Transferase.
FT   CHAIN           1..431
FT                   /note="Levansucrase"
FT                   /id="PRO_0000057719"
FT   ACT_SITE        62
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        303
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            219
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
SQ   SEQUENCE   431 AA;  47603 MW;  795FEA246D40C40F CRC64;
     MSTSSSAVSQ LKNSPLAGNI NYEPTVWSRA DALKVNENDP TTTQPLVSAD FPVMSDTVFI
     WDTMPLRELD GTVVSVNGWS VILTLTADRH PNDPQYLDAN GRYDIKRDWE DRHGRARMSY
     WYSRTGKDWI FGGRVMAEGV SPTTREWAGT PILLNDKGDI DLYYTCVTPG AAIAKVRGRI
     VTSDQGVELK DFTQVKKLFE ADGTYYQTEA QNSSWNFRDP SPFIDPNDGK LYMVFEGNVA
     GERGSHTVGA AELGPVPPGH EDVGGARFQV GCIGLAVAKD LSGEEWEILP PLVTAVGVND
     QTERPHYVFQ DGKYYLFTIS HKFTYAEGLT GPDGVYGFVG EHLFGPYRPM NASGLVLGNP
     PEQPFQTYSH CVMPNGLVTS FIDSVPTEGE DYRIGGTEAP TVRILLKGDR SFVQEEYDYG
     YIPAMKDVTL S