SACB_RAHAC
ID SACB_RAHAC Reviewed; 415 AA.
AC O54435; H2J2C5;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Levansucrase;
DE EC=2.4.1.10;
DE AltName: Full=Beta-D-fructofuranosyl transferase;
DE AltName: Full=Sucrose 6-fructosyl transferase;
GN Name=sacB; OrderedLocusNames=Rahaq2_5035;
OS Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 /
OS NCIMB 13365 / CIP 78.65).
OG Plasmid pRahaq202.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Rahnella.
OX NCBI_TaxID=745277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP
RC 78.65;
RX PubMed=9928133; DOI=10.1111/j.1749-6632.1998.tb10369.x;
RA Song K.B., Seo J.W., Kim M.G., Rhee S.K.;
RT "Levansucrase of Rahnella aquatilis ATCC33071. Gene cloning, expression,
RT and levan formation.";
RL Ann. N. Y. Acad. Sci. 864:506-511(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP
RC 78.65; PLASMID=pRahaq202;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Held B., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Sobecky P.,
RA Martinez R., Woyke T.;
RT "Complete sequence of plasmid 2 of Rahnella aquatilis CIP 78.65.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside +
CC sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-
CC glucopyranoside + D-glucose; Xref=Rhea:RHEA:13653, Rhea:RHEA-
CC COMP:13093, Rhea:RHEA-COMP:13094, ChEBI:CHEBI:4167,
CC ChEBI:CHEBI:17992, ChEBI:CHEBI:134464; EC=2.4.1.10;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR EMBL; U91484; AAC36458.1; -; Genomic_DNA.
DR EMBL; CP003246; AEX54742.1; -; Genomic_DNA.
DR RefSeq; WP_014333851.1; NZ_JMPO01000160.1.
DR AlphaFoldDB; O54435; -.
DR SMR; O54435; -.
DR STRING; 745277.GRAQ_04881; -.
DR CAZy; GH68; Glycoside Hydrolase Family 68.
DR EnsemblBacteria; AEX54742; AEX54742; Rahaq2_5035.
DR KEGG; raq:Rahaq2_5035; -.
DR PATRIC; fig|745277.3.peg.4810; -.
DR eggNOG; COG1621; Bacteria.
DR HOGENOM; CLU_031862_1_0_6; -.
DR OMA; DPWFFED; -.
DR BRENDA; 2.4.1.10; 5269.
DR Proteomes; UP000009010; Plasmid pRahaq202.
DR GO; GO:0050053; F:levansucrase activity; IEA:UniProtKB-EC.
DR GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR CDD; cd08997; GH68; 1.
DR Gene3D; 2.115.10.20; -; 1.
DR InterPro; IPR003469; Glyco_hydro_68.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR Pfam; PF02435; Glyco_hydro_68; 1.
DR SUPFAM; SSF75005; SSF75005; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Plasmid; Transferase.
FT CHAIN 1..415
FT /note="Levansucrase"
FT /id="PRO_0000057720"
FT ACT_SITE 46
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q74K42"
FT ACT_SITE 287
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q74K42"
FT SITE 203
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:Q74K42"
SQ SEQUENCE 415 AA; 45939 MW; E028828813D13A74 CRC64;
MTNLNYTPTI WTRADALKVN ENDPTTTQPI VDADFPVMSD EVFIWDTMPL RSLDGTVVSV
DGWSVIFTLT AQRNNNNSEY LDAEGNYDIT SDWNNRHGRA RICYWYSRTG KDWIFGGRVM
AEGVSPTSRE WAGTPILLNE DGDIDLYYTC VTPGATIAKV RGKVLTSEEG VTLAGFNEVK
SLFSADGVYY QTESQNPYWN FRDPSPFIDP HDGKLYMVFE GNVAGERGSH VIGKQEMGTL
PPGHRDVGNA RYQAGCIGMA VAKDLSGDEW EILPPLVTAV GVNDQTERPH FVFQDGKYYL
FTISHKFTYA DGLTGPDGVY GFLSDNLTGP YSPMNGSGLV LGNPPSQPFQ TYSHCVMPNG
LVTSFIDNVP TSDGNYRIGG TEAPTVKIVL KGNRSFVERV FDYGYIPPMK NIILN
