ID   SACB_ZYMMA              Reviewed;         423 AA.
AC   F8DT26; Q06487; Q5NQK6; Q60114; Q60116;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Levansucrase;
DE            EC=2.4.1.10;
DE   AltName: Full=Beta-D-fructofuranosyl transferase;
DE   AltName: Full=Sucrose 6-fructosyl transferase;
GN   Name=sacB; Synonyms=levU, sucE2; OrderedLocusNames=Zmob_0914;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 /
OS   NCIMB 8938 / NRRL B-806 / ZM1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=555217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=8318541; DOI=10.1016/0167-4781(93)90130-6;
RA   Song K.B., Joo H.K., Rhee S.-K.;
RT   "Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1
RT   (ATCC10988).";
RL   Biochim. Biophys. Acta 1173:320-324(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RA   Gunasekaran P., Mukundan G., Kannan R., Velmurugan S., Ait-Abdelkader N.,
RA   Alvarez E., Baratti J.;
RL   Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=21725006; DOI=10.1128/jb.05395-11;
RA   Pappas K.M., Kouvelis V.N., Saunders E., Brettin T.S., Bruce D., Detter C.,
RA   Balakireva M., Han C.S., Savvakis G., Kyrpides N.C., Typas M.A.;
RT   "Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis
RT   lectotype strain ATCC 10988.";
RL   J. Bacteriol. 193:5051-5052(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[6)-beta-D-fructofuranosyl-(2->](n) alpha-D-glucopyranoside +
CC         sucrose = [6)-beta-D-fructofuranosyl-(2->](n+1) alpha-D-
CC         glucopyranoside + D-glucose; Xref=Rhea:RHEA:13653, Rhea:RHEA-
CC         COMP:13093, Rhea:RHEA-COMP:13094, ChEBI:CHEBI:4167,
CC         ChEBI:CHEBI:17992, ChEBI:CHEBI:134464; EC=2.4.1.10;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- PTM: Does not seem to be N-terminally processed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR   EMBL; AF081588; AAA27695.1; -; Genomic_DNA.
DR   EMBL; L33402; AAA27702.1; -; Genomic_DNA.
DR   EMBL; CP002850; AEH62749.1; -; Genomic_DNA.
DR   PIR; S33771; S33771.
DR   RefSeq; WP_011240294.1; NC_017262.1.
DR   AlphaFoldDB; F8DT26; -.
DR   SMR; F8DT26; -.
DR   STRING; 555217.Zmob_0914; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   EnsemblBacteria; AEH62749; AEH62749; Zmob_0914.
DR   GeneID; 58026222; -.
DR   KEGG; zmm:Zmob_0914; -.
DR   eggNOG; COG1621; Bacteria.
DR   HOGENOM; CLU_031862_1_0_5; -.
DR   OMA; DPWFFED; -.
DR   Proteomes; UP000001494; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050053; F:levansucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Secreted; Transferase.
FT   CHAIN           1..423
FT                   /note="Levansucrase"
FT                   /id="PRO_0000414233"
FT   ACT_SITE        48
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        278
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            194
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   CONFLICT        200..203
FT                   /note="NPED -> TPKI (in Ref. 1; AAA27695)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   423 AA;  46762 MW;  19A4691DA3EB9FE3 CRC64;
     MLNKAGIAEP SLWTRADAMK VHTDDPTATM PTIDYDFPVM TDKYWVWDTW PLRDINGQVV
     SFQGWSVIFA LVADRTKYGW HNRNDGARIG YFYSRGGSNW IFGGHLLKDG ANPRSWEWSG
     CTIMAPGTAN SVEVFFTSVN DTPSESVPAQ CKGYIYADDK SVWFDGFDKV TDLFQADGLY
     YADYAENNFW DFRDPHVFIN PEDGKTYALF EGNVAMERGT VAVGEEEIGP VPPKTETPDG
     ARYCAAAIGI AQALNEARTE WKLLPPLVTA FGVNDQTERP HVVFQNGLTY LFTISHHSTY
     ADGLSGPDGV YGFVSENGIF GPYEPLNGSG LVLGNPSSQP YQAYSHYVMT NGLVTSFIDT
     IPSSDPNVYR YGGTLAPTIK LELVGHRSFV TEVKGYGYIP PQIEWLAEDE SSNSAAALSL
     LNK