SACT_BACSU
ID SACT_BACSU Reviewed; 276 AA.
AC P26212;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=SacPA operon antiterminator;
GN Name=sacT; OrderedLocusNames=BSU38070; ORFNames=ipa-47d;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2163394; DOI=10.1128/jb.172.7.3966-3973.1990;
RA Debarbouille M., Arnaud M., Fouet A., Klier A., Rapoport G.;
RT "The sacT gene regulating the sacPA operon in Bacillus subtilis shares
RT strong homology with transcriptional antiterminators.";
RL J. Bacteriol. 172:3966-3973(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7934828; DOI=10.1111/j.1365-2958.1993.tb01963.x;
RA Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F.,
RA Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E.,
RA Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A.;
RT "Bacillus subtilis genome project: cloning and sequencing of the 97 kb
RT region from 325 degrees to 333 degrees.";
RL Mol. Microbiol. 10:371-384(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Mediates positive regulation of the sacPA operon by
CC functioning as an antiterminator factor of transcription.
CC -!- PTM: Phosphorylated and inactivated by SacP (EII-Scr). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the transcriptional antiterminator BglG family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA22726.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J03006; AAA22726.1; ALT_INIT; Genomic_DNA.
DR EMBL; X73124; CAA51603.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15833.1; -; Genomic_DNA.
DR PIR; S39702; S39702.
DR RefSeq; NP_391686.1; NC_000964.3.
DR RefSeq; WP_003243088.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P26212; -.
DR SMR; P26212; -.
DR STRING; 224308.BSU38070; -.
DR PaxDb; P26212; -.
DR PRIDE; P26212; -.
DR EnsemblBacteria; CAB15833; CAB15833; BSU_38070.
DR GeneID; 937284; -.
DR KEGG; bsu:BSU38070; -.
DR PATRIC; fig|224308.179.peg.4121; -.
DR eggNOG; COG3711; Bacteria.
DR OMA; YQRLLTH; -.
DR PhylomeDB; P26212; -.
DR BioCyc; BSUB:BSU38070-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.30.24.10; -; 1.
DR InterPro; IPR004341; CAT_RNA-bd_dom.
DR InterPro; IPR036650; CAT_RNA-bd_dom_sf.
DR InterPro; IPR011608; PRD.
DR InterPro; IPR036634; PRD_sf.
DR InterPro; IPR001550; Transcrpt_antitermin_CS.
DR Pfam; PF03123; CAT_RBD; 1.
DR Pfam; PF00874; PRD; 2.
DR SMART; SM01061; CAT_RBD; 1.
DR SUPFAM; SSF50151; SSF50151; 1.
DR SUPFAM; SSF63520; SSF63520; 2.
DR PROSITE; PS00654; PRD_1; 1.
DR PROSITE; PS51372; PRD_2; 2.
PE 3: Inferred from homology;
KW Activator; Phosphoprotein; Reference proteome; Repeat; RNA-binding;
KW Transcription; Transcription regulation.
FT CHAIN 1..276
FT /note="SacPA operon antiterminator"
FT /id="PRO_0000204248"
FT DOMAIN 62..167
FT /note="PRD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT DOMAIN 168..276
FT /note="PRD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT VARIANT 96
FT /note="D -> Y (in sacT30 mutant)"
SQ SEQUENCE 276 AA; 32074 MW; F1D63E4BC7CFBA03 CRC64;
MKIYKVLNNN AALIKEDDQE KIVMGPGIAF QKKKNDLIPM NKVEKIFVVR DENEKFKQIL
QTLPEEHIEI AEDIISYAEG ELAAPLSDHI HIALSDHLSF AIERIQNGLL VQNKLLHEIK
ALYKKEYEIG LWAIGHVKET LGVSLPEDEA GYIALHIHTA KMDAESMYSA LKHTTMIKEM
IEKIKQYFNR KVDENSISYQ RLVTHLRYAV SRLESNEALH RMDEEMLYFI QKKYSFAYQC
ALELAEFLKN EYQLHLPESE AGYITLHVQR LQDLSE
