SAD2H_ARATH
ID SAD2H_ARATH Reviewed; 1030 AA.
AC F4J738; B9DFH6; Q9LYT4;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Importin beta-like SAD2 homolog {ECO:0000305};
GN OrderedLocusNames=At3g59020 {ECO:0000312|Araport:AT3G59020};
GN ORFNames=F17J16.70 {ECO:0000312|EMBL:CAB86930.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf {ECO:0000312|EMBL:BAH19493.1};
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=16889648; DOI=10.1111/j.1365-313x.2006.02833.x;
RA Verslues P.E., Guo Y., Dong C.H., Ma W., Zhu J.K.;
RT "Mutation of SAD2, an importin beta-domain protein in Arabidopsis, alters
RT abscisic acid sensitivity.";
RL Plant J. 47:776-787(2006).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Functions probably in nuclear protein import, either by
CC acting as autonomous nuclear transport receptor or as an adapter-like
CC protein in association with other importin subunits.
CC {ECO:0000250|UniProtKB:F4IRR2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:F4IRR2}. Nucleus
CC {ECO:0000250|UniProtKB:F4IRR2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoform are produced. According to EST sequences.
CC {ECO:0000305};
CC Name=1;
CC IsoId=F4J738-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:16889648}.
CC -!- SIMILARITY: Belongs to the importin beta family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB86930.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL163527; CAB86930.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79862.1; -; Genomic_DNA.
DR EMBL; AK316774; BAH19493.1; -; mRNA.
DR PIR; T47784; T47784.
DR RefSeq; NP_001030888.1; NM_001035811.2. [F4J738-1]
DR AlphaFoldDB; F4J738; -.
DR SMR; F4J738; -.
DR STRING; 3702.AT3G59020.2; -.
DR iPTMnet; F4J738; -.
DR PaxDb; F4J738; -.
DR PRIDE; F4J738; -.
DR ProteomicsDB; 232728; -. [F4J738-1]
DR EnsemblPlants; AT3G59020.2; AT3G59020.2; AT3G59020. [F4J738-1]
DR GeneID; 825071; -.
DR Gramene; AT3G59020.2; AT3G59020.2; AT3G59020. [F4J738-1]
DR KEGG; ath:AT3G59020; -.
DR Araport; AT3G59020; -.
DR TAIR; locus:2077715; AT3G59020.
DR eggNOG; KOG1991; Eukaryota.
DR HOGENOM; CLU_004196_0_0_1; -.
DR InParanoid; F4J738; -.
DR PhylomeDB; F4J738; -.
DR PRO; PR:F4J738; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J738; baseline and differential.
DR Genevisible; F4J738; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR013713; XPO2_central.
DR Pfam; PF08506; Cse1; 1.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..1030
FT /note="Importin beta-like SAD2 homolog"
FT /id="PRO_0000431578"
FT DOMAIN 25..99
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REGION 886..928
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 940..964
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 888..921
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 944..963
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 587
FT /note="A -> T (in Ref. 3; BAH19493)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1030 AA; 117769 MW; E37DDA2E9EAA06CB CRC64;
MDLPSLALIV GAAAFSPNPD ERRAAEQSLN QLQHTPQHLI RILQIIVDGG SDLSVRQSAS
IHFKNFIAKH WEPHSGDQNI ILPSDKNVVR NQILVFVSQV PPILRVQMGE CLKTIIYADY
PEQWPELLDW VKQNLQKPQV YGALFVLRIL SSKYEFKSDE DRAPIHRVVE ETFPHLLNIF
NNLVHVENPS LEVADHIKLI CKIFWSCIYL ELPRPLFDPN FFNAWMGLFL NILERPVPVE
GQPEDPELRK SWGWWKAKKW IAHILNRLYT RFGDLKLQNP DNKAFAQMFQ INYAAKILEC
HLKLLNAIRI GGYLPDRVIN LILQYLSNSI SKSSMYNLLQ PHLNTLLFEI VFPLMCFNDN
DQMLWDEDPH EYVRKGYDII EDLYSPRTAS MDFVTELVRK RGKENFPKFI QFVVDIFKRY
NEASLENKPY RLKDGALLAV GTLCDKLRQT EPYKSELENM LVQHVFPEFS SPAGHLRAKA
AWVAGQYANI DFSDQSNFSK ALHCVISGMC DLELPVRVDS VFALRSFIEA CKDLDEIRPV
LPQLLDEFFK LMKEVENEDL AFTLETIVYK FGEEISPYAL GLCQNLASAF WRCIDTDNGD
DETDDAGALA AVGCLRAIST ILESISSLPH LYGQIEPQLL PIMRKMLTTD GQDVFEEVLE
IVSYITTFSP TISLEMWSLW PLMMEALVDW AIDFFPNILV PLHNYISRGT GHYLTCKEPD
YQQNLWNVIS VLMANKNIDD SDLEPAPKLL GIVLQTCKGQ VDQWVEPYLR ITLDRLRGAE
KSSFKCLLVE VVANAFYYNT PLALGILQRF GIATEIFTLW FQMLQEKKKS GARSNFKREH
DKKVCILGLT SLFSLPAGQL PGEVLPHVFR ALLELLVAYK DQLAEAAKAE EEEEDEDGDD
DDMDEFQTDD EDEDGDDENP DETDGSTLRK LAAQAKDFRS YSDDDDFSDD DFSDDEELES
PIDEVDPFVL FMDAVTAMQV SDSPRFQSLT QTLDPHYHGL ASTIAQHTEL RRAEILKEKL
EKQSSATVAS
