ID   SADH_MYCTU              Reviewed;         276 AA.
AC   P9WGP9; F2GNY3; L0TEA6; O53302; Q7D656;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Putative oxidoreductase SadH;
DE            EC=1.-.-.-;
GN   Name=sadH; OrderedLocusNames=Rv3085;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION, AND GENE NAME.
RX   PubMed=14568148; DOI=10.1016/s0378-1097(03)00648-7;
RA   Singh A., Jain S., Gupta S., Das T., Tyagi A.K.;
RT   "mymA operon of Mycobacterium tuberculosis: its regulation and importance
RT   in the cell envelope.";
RL   FEMS Microbiol. Lett. 227:53-63(2003).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Erdman;
RX   PubMed=15937179; DOI=10.1128/jb.187.12.4173-4186.2005;
RA   Singh A., Gupta R., Vishwakarma R.A., Narayanan P.R., Paramasivan C.N.,
RA   Ramanathan V.D., Tyagi A.K.;
RT   "Requirement of the mymA operon for appropriate cell wall ultrastructure
RT   and persistence of Mycobacterium tuberculosis in the spleens of guinea
RT   pigs.";
RL   J. Bacteriol. 187:4173-4186(2005).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16893682; DOI=10.1016/j.tube.2006.01.021;
RA   Cheruvu M., Plikaytis B.B., Shinnick T.M.;
RT   "The acid-induced operon Rv3083-Rv3089 is required for growth of
RT   Mycobacterium tuberculosis in macrophages.";
RL   Tuberculosis 87:12-20(2007).
CC   -!- FUNCTION: Required for maintaining the appropriate mycolic acid
CC       composition and permeability of the envelope on its exposure to acidic
CC       pH. {ECO:0000269|PubMed:15937179}.
CC   -!- INDUCTION: Expression is controlled by VirS. Induced at acidic pH and
CC       in macrophages. {ECO:0000269|PubMed:14568148}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation of the mymA operon causes altered
CC       cell wall structure, reduced contents and altered composition of
CC       mycolic acids along with the accumulation of saturated C24 and C26
CC       fatty acids, and enhanced susceptibility to antibiotics, detergents and
CC       acidic pH. Also impairs ability to survive in macrophages.
CC       {ECO:0000269|PubMed:15937179, ECO:0000269|PubMed:16893682}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45894.1; -; Genomic_DNA.
DR   PIR; A70853; A70853.
DR   RefSeq; NP_217601.1; NC_000962.3.
DR   RefSeq; WP_003416074.1; NZ_NVQJ01000011.1.
DR   AlphaFoldDB; P9WGP9; -.
DR   SMR; P9WGP9; -.
DR   STRING; 83332.Rv3085; -.
DR   PaxDb; P9WGP9; -.
DR   DNASU; 888656; -.
DR   GeneID; 888656; -.
DR   KEGG; mtu:Rv3085; -.
DR   TubercuList; Rv3085; -.
DR   eggNOG; COG4221; Bacteria.
DR   OMA; EWEQMID; -.
DR   PhylomeDB; P9WGP9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0051701; P:biological process involved in interaction with host; IMP:MTBBASE.
DR   GO; GO:0010447; P:response to acidic pH; IEP:MTBBASE.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..276
FT                   /note="Putative oxidoreductase SadH"
FT                   /id="PRO_0000420878"
FT   ACT_SITE        155
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   276 AA;  29217 MW;  4721366EEBE3E270 CRC64;
     MSSFEGKVAV ITGAGSGIGR ALALNLSEKR AKLALSDVDT DGLAKTVRLA QALGAQVKSD
     RLDVAEREAV LAHADAVVAH FGTVHQVYNN AGIAYNGNVD KSEFKDIERI IDVDFWGVVN
     GTKAFLPHVI ASGDGHIVNI SSLFGLIAVP GQSAYNAAKF AVRGFTEALR QEMLVARHPV
     KVTCVHPGGI KTAVARNATV ADGEDQQTFA EFFDRRLALH SPEMAAKTIV NGVAKGQARV
     VVGLEAKAVD VLARIMGSSY QRLVAAGVAK FFPWAK