ID   SAE1_DANRE              Reviewed;         348 AA.
AC   Q6IQS6;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=SUMO-activating enzyme subunit 1;
DE   AltName: Full=Ubiquitin-like 1-activating enzyme E1A;
GN   Name=sae1; Synonyms=sae2a, uble1a; ORFNames=zgc:86633;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The heterodimer acts as an E1 ligase for sumo1, sumo2, and
CC       sumo3. It mediates ATP-dependent activation of sumo proteins followed
CC       by formation of a thioester bond between a sumo protein and a conserved
CC       active site cysteine residue on uba2/sae2 (By similarity).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein sumoylation.
CC   -!- SUBUNIT: Heterodimer of sae1 and uba2/sae2. The heterodimer corresponds
CC       to the two domains that are encoded on a single polypeptide chain in
CC       ubiquitin-activating enzyme E1. Interacts with ube2i (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC071328; AAH71328.1; -; mRNA.
DR   RefSeq; NP_001002058.1; NM_001002058.1.
DR   AlphaFoldDB; Q6IQS6; -.
DR   SMR; Q6IQS6; -.
DR   STRING; 7955.ENSDARP00000016138; -.
DR   PaxDb; Q6IQS6; -.
DR   PRIDE; Q6IQS6; -.
DR   Ensembl; ENSDART00000011447; ENSDARP00000016138; ENSDARG00000010487.
DR   GeneID; 415148; -.
DR   KEGG; dre:415148; -.
DR   CTD; 10055; -.
DR   ZFIN; ZDB-GENE-040625-21; sae1.
DR   eggNOG; KOG2014; Eukaryota.
DR   GeneTree; ENSGT00550000075007; -.
DR   HOGENOM; CLU_002556_4_0_1; -.
DR   InParanoid; Q6IQS6; -.
DR   OMA; TDVWGTF; -.
DR   OrthoDB; 1180926at2759; -.
DR   PhylomeDB; Q6IQS6; -.
DR   TreeFam; TF315037; -.
DR   Reactome; R-DRE-3065676; SUMO is conjugated to E1 (UBA2:SAE1).
DR   Reactome; R-DRE-3065678; SUMO is transferred from E1 to E2 (UBE2I, UBC9).
DR   UniPathway; UPA00886; -.
DR   PRO; PR:Q6IQS6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 15.
DR   Bgee; ENSDARG00000010487; Expressed in gastrula and 26 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0031510; C:SUMO activating enzyme complex; ISS:UniProtKB.
DR   GO; GO:0008022; F:protein C-terminus binding; ISS:UniProtKB.
DR   GO; GO:0019948; F:SUMO activating enzyme activity; IBA:GO_Central.
DR   GO; GO:0060216; P:definitive hemopoiesis; IMP:ZFIN.
DR   GO; GO:0061484; P:hematopoietic stem cell homeostasis; IMP:ZFIN.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0016925; P:protein sumoylation; IMP:ZFIN.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   InterPro; IPR000011; UBQ/SUMO-activ_enz_E1-like.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PRINTS; PR01849; UBIQUITINACT.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   2: Evidence at transcript level;
KW   Ligase; Nucleus; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..348
FT                   /note="SUMO-activating enzyme subunit 1"
FT                   /id="PRO_0000268869"
SQ   SEQUENCE   348 AA;  39076 MW;  549F97AC8D394BCE CRC64;
     MIDTIEKEDT IISEEEAAQY DRQIRLWGLD AQKRLRGSRV LLVGLRGLGA EVAKNLILAG
     VKGLTLLDHE QVTEESRRAQ FLIPVDADGQ NHAQASLERA QFLNPMVEVK ADTEPVESKP
     DDFFFQFDAV CLTRCSRDLM VRVDQLCASR NIKVFCGDVY GYNGYMFSDL GQEYHYVEEK
     PKVVKGSNEA NDGPEAKKPK IDPNETTMVK KTISFCSLKE ALEVDWTTEK AKSSLKRIPA
     DYFLLQVLLK FRTDKGRDPQ PDSFAEDSQL LLQIRDDVLE TMGLSSDLLP NTFVSYCFSE
     MSPVCAVVGG VLGQEIVKAL SQRDAPHRNF FFFDGLKGSG VVDYFSSK