ID   SAE1_DICDI              Reviewed;         330 AA.
AC   Q54WI4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=SUMO-activating enzyme subunit 1;
DE   AltName: Full=Ubiquitin-like 1-activating enzyme E1A;
GN   Name=sae1; Synonyms=uble1a; ORFNames=DDB_G0279641;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: The dimeric enzyme acts as an E1 ligase for sumo. It mediates
CC       ATP-dependent activation of sumo and formation of a thioester with a
CC       conserved cysteine residue on sae2 (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein sumoylation.
CC   -!- SUBUNIT: Heterodimer of sae1 and sae2. The complex binds sumo via sae2
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000032; EAL67595.1; -; Genomic_DNA.
DR   RefSeq; XP_641569.1; XM_636477.1.
DR   AlphaFoldDB; Q54WI4; -.
DR   SMR; Q54WI4; -.
DR   STRING; 44689.DDB0302359; -.
DR   PaxDb; Q54WI4; -.
DR   EnsemblProtists; EAL67595; EAL67595; DDB_G0279641.
DR   GeneID; 8622144; -.
DR   KEGG; ddi:DDB_G0279641; -.
DR   dictyBase; DDB_G0279641; sae1.
DR   eggNOG; KOG2014; Eukaryota.
DR   HOGENOM; CLU_002556_4_1_1; -.
DR   InParanoid; Q54WI4; -.
DR   OMA; TDVWGTF; -.
DR   PhylomeDB; Q54WI4; -.
DR   Reactome; R-DDI-3065676; SUMO is conjugated to E1 (UBA2:SAE1).
DR   Reactome; R-DDI-3065678; SUMO is transferred from E1 to E2 (UBE2I, UBC9).
DR   UniPathway; UPA00886; -.
DR   PRO; PR:Q54WI4; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0031510; C:SUMO activating enzyme complex; ISS:dictyBase.
DR   GO; GO:0019948; F:SUMO activating enzyme activity; ISS:dictyBase.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0016925; P:protein sumoylation; ISS:dictyBase.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   InterPro; IPR000011; UBQ/SUMO-activ_enz_E1-like.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PRINTS; PR01849; UBIQUITINACT.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..330
FT                   /note="SUMO-activating enzyme subunit 1"
FT                   /id="PRO_0000328138"
SQ   SEQUENCE   330 AA;  37227 MW;  D928C96E8461169F CRC64;
     MTNPENTPMT TTNEGGKGLT EYEAKIYDRS IRLWGVDAQA KLRQSKVLFI GINGLMSEII
     KNVVLAGVDS ITLVDDHIIT TSDLSAHLFI NEDSVGKVIS TESVFAISEL NPLVTIDVYD
     KEIETMDDQF IKNYTMVVIS DKNLNNVSKV NSLCRKNNVS FIFSHSFGLK GLFFSDLNEF
     KYFTKTTTEP PKTETHISIF KSFKESMGYD WSKTNSRTPL PFFALSTLYQ FEEKHNRVPD
     NISDSDLSEL KSIINSSIEK FNLKNTDSNK YFEETKDLLN KMNIEISPVC AIVGGIVGAE
     IIKIITQNMQ VLNNFFFYDG VKGTGLVEQF