SAE3_YEAST
ID SAE3_YEAST Reviewed; 91 AA.
AC P89114; D3DL30;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Pachytene arrest protein SAE3;
DE AltName: Full=Sporulation in the absence of SPO11 protein 3;
GN Name=SAE3; OrderedLocusNames=YHR079C-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX PubMed=9215889; DOI=10.1093/genetics/146.3.817;
RA McKee A.H.Z., Kleckner N.;
RT "Mutations in Saccharomyces cerevisiae that block meiotic prophase
RT chromosome metabolism and confer cell cycle arrest at pachytene identify
RT two new meiosis-specific genes SAE1 and SAE3.";
RL Genetics 146:817-834(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MEI5 AND DMC1, AND
RP REVISION OF GENE MODEL.
RX PubMed=15620352; DOI=10.1016/j.cell.2004.10.031;
RA Hayase A., Takagi M., Miyazaki T., Oshiumi H., Shinohara M., Shinohara A.;
RT "A protein complex containing Mei5 and Sae3 promotes the assembly of the
RT meiosis-specific RecA homolog Dmc1.";
RL Cell 119:927-940(2004).
RN [5]
RP FUNCTION.
RX PubMed=15238514; DOI=10.1534/genetics.103.023762;
RA Young J.A., Hyppa R.W., Smith G.R.;
RT "Conserved and nonconserved proteins for meiotic DNA breakage and repair in
RT yeasts.";
RL Genetics 167:593-605(2004).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MEI5.
RX PubMed=15579681; DOI=10.1534/genetics.103.025700;
RA Tsubouchi H., Roeder G.S.;
RT "The budding yeast mei5 and sae3 proteins act together with dmc1 during
RT meiotic recombination.";
RL Genetics 168:1219-1230(2004).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16341884; DOI=10.1007/s00438-005-0078-4;
RA Fukuda T., Ohya Y.;
RT "Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break
RT repair site initiated by meiosis-specific endonuclease VDE (PI-SceI).";
RL Mol. Genet. Genomics 275:204-214(2006).
CC -!- FUNCTION: Involved in meiotic DNA-break repair. Required for the
CC recruitment of DCM1 to meiosis recombination hot spots.
CC {ECO:0000269|PubMed:15238514, ECO:0000269|PubMed:15579681,
CC ECO:0000269|PubMed:15620352, ECO:0000269|PubMed:16341884,
CC ECO:0000269|PubMed:9215889}.
CC -!- SUBUNIT: Forms a ternary complex with DMC1 and MEI5.
CC -!- INTERACTION:
CC P89114; P32489: MEI5; NbExp=4; IntAct=EBI-16412526, EBI-10685;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15579681,
CC ECO:0000269|PubMed:15620352, ECO:0000269|PubMed:16341884}.
CC Note=Localizes at chromosomal foci at leptotene and zigotene.
CC -!- INDUCTION: During meiosis. {ECO:0000269|PubMed:9215889}.
CC -!- SIMILARITY: Belongs to the SWI5/SAE3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40146.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAB68897.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U82546; AAB40146.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U10556; AAB68897.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006934; DAA06774.1; -; Genomic_DNA.
DR PIR; S72456; S72456.
DR RefSeq; NP_011947.2; NM_001180026.1.
DR AlphaFoldDB; P89114; -.
DR SMR; P89114; -.
DR BioGRID; 36514; 30.
DR ComplexPortal; CPX-1288; MEI5-SAE3 DMC1 recombinase assembly factor complex.
DR DIP; DIP-5100N; -.
DR IntAct; P89114; 2.
DR STRING; 4932.YHR079C-A; -.
DR iPTMnet; P89114; -.
DR PaxDb; P89114; -.
DR PRIDE; P89114; -.
DR EnsemblFungi; YHR079C-A_mRNA; YHR079C-A; YHR079C-A.
DR GeneID; 856479; -.
DR KEGG; sce:YHR079C-A; -.
DR SGD; S000001957; SAE3.
DR VEuPathDB; FungiDB:YHR079C-A; -.
DR eggNOG; ENOG502S9IK; Eukaryota.
DR HOGENOM; CLU_106110_2_0_1; -.
DR InParanoid; P89114; -.
DR OMA; EYESMNG; -.
DR BioCyc; YEAST:G3O-31241-MON; -.
DR PRO; PR:P89114; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P89114; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0000228; C:nuclear chromosome; IDA:ComplexPortal.
DR GO; GO:0032798; C:Swi5-Sfr1 complex; IBA:GO_Central.
DR GO; GO:0034974; C:Swi5-Swi2 complex; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0000707; P:meiotic DNA recombinase assembly; IMP:SGD.
DR GO; GO:0010772; P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0000709; P:meiotic joint molecule formation; IBA:GO_Central.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR InterPro; IPR010760; DNA-repair_Swi5.
DR Pfam; PF07061; Swi5; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..91
FT /note="Pachytene arrest protein SAE3"
FT /id="PRO_0000269645"
SQ SEQUENCE 91 AA; 10827 MW; 0EAD012E48D482AA CRC64;
MNYLETQLNK KQKQIQEYES MNGNLIKMFE QLSKEKKNDE TPKKISSTYI KELKEYNELR
DAGLRLAQII ADEKQCKIKD VFEEIGYSMK D
