SAEA_MYCS2
ID SAEA_MYCS2 Reviewed; 692 AA.
AC A0QNH4; I7FCB7;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Putative ESX-1 scaffolding and assembly protein SaeA;
GN Name=saeA {ECO:0000303|PubMed:22233444};
GN OrderedLocusNames=MSMEG_0044, MSMEI_0046;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155, and MKD8;
RX PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT Mycobacterium smegmatis.";
RL Mol. Microbiol. 69:794-808(2008).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA Gray T.A., Derbyshire K.M.;
RT "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT secretory apparatus in mycobacteria.";
RL Mol. Microbiol. 83:654-664(2012).
CC -!- FUNCTION: May be involved in assembly of the ESX-1 / type VII
CC specialized secretion system (T7SS), which exports several proteins
CC including EsxA and EsxB (PubMed:22233444). Involved in DNA conjugation
CC in recipient (MKD8) but not donor (mc(2)155) strain (PubMed:18554329).
CC {ECO:0000269|PubMed:18554329, ECO:0000305|PubMed:22233444}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22233444}.
CC Note=Localizes at or near the cell pole in (on average) 1 discrete
CC spot. Polar localization does not require ESX-1 genes
CC (PubMed:22233444). {ECO:0000269|PubMed:22233444}.
CC -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC recipient strain (MKD8), no effect when disrupted in donor strain
CC (mc(2)155) (PubMed:18554329). The recipient strain does not secrete
CC EsxB (PubMed:18554329). Disruption of the probable saeA-saeB-saeC
CC operon completely blocks polar localization of EccCb1
CC (PubMed:22233444). {ECO:0000269|PubMed:18554329,
CC ECO:0000269|PubMed:22233444}.
CC -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC distinct donor and recipient strains; mc(2)155 is a donor strain while
CC MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC transfer do not always alter DNA transfer in a recipient strain.
CC {ECO:0000305|PubMed:18554329}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFP36528.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000480; ABK72207.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36528.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_011726628.1; NZ_SIJM01000001.1.
DR RefSeq; YP_884462.1; NC_008596.1.
DR AlphaFoldDB; A0QNH4; -.
DR SMR; A0QNH4; -.
DR STRING; 246196.MSMEI_0046; -.
DR EnsemblBacteria; ABK72207; ABK72207; MSMEG_0044.
DR EnsemblBacteria; AFP36528; AFP36528; MSMEI_0046.
DR GeneID; 66738234; -.
DR KEGG; msg:MSMEI_0046; -.
DR KEGG; msm:MSMEG_0044; -.
DR PATRIC; fig|246196.19.peg.42; -.
DR eggNOG; ENOG50332MG; Bacteria.
DR OMA; WSRAYFT; -.
DR OrthoDB; 143521at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE 4: Predicted;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..692
FT /note="Putative ESX-1 scaffolding and assembly protein
FT SaeA"
FT /id="PRO_0000438351"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 87..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..104
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 692 AA; 74978 MW; D77990E63C1D1A0F CRC64;
MGERGELVSD LHPSDDHDAD PRLAPLLAWR QQLVDSGAVA PRSFKEAHLR LVLRSGRTDV
EQIRAMLPGS VAEHAEEMAR ILAELTPAAP EPDPPPVPEP QPEPEPGPGK HRSPETDEPP
VATTTEIPIP TTGFAPFQFS SQQVALHDIT VQRTDAAVEL SWPPYEAPED EAAQDISVVM
YRVVSSDDQA PYSPDPAHLV ALTEEPKATD ERQQVSPVRH YQVWVNVGAS EAAARKTQPV
LYATAVLVRP VTGFEIREDA GWVIGQWTAP PGVTAVHVFR VPIDEVDRDE AQYRILTAGE
NLAGFVDTEP VRGQRYRYRA RCAVNVDGVV RLSEAAEADV ELAAALMPVT DLVVETAADG
ASCDLSWTPP AGGQVAIYRS QNGPSADAEA IELPQGALEQ VGLTPELRVT QDLTEETGSD
GRRRARLTGV TWPSEWSRAY FTPVTLMGER AMLGRTLSSV RTGTIRDIEL AEYCNKQVLT
FDWPDGAASV IVYLAPKGHD PRSGLNGRSF EISLEEYERY GGMHLTGQLP VGGCSLHLAP
VAFAGGRRVV GAFSSIEYRG LLRLQYAVRI GRDPNGFPTT ATIALRAEQN VPGSPGFVLV
NNPQRLPLSV HDGHPVDVAP LDARGQLADH PSKELRWSAL TTSGDGELWA ANLSGLQGWI
RLFVNIGSPA QLRVIALLDP PVETLRLTAA TL
