ID   SAEA_MYCSE              Reviewed;         692 AA.
AC   L8FM21; A0A2U9PH32;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Putative ESX-1 scaffolding and assembly protein SaeA;
GN   Name=saeA;
GN   ORFNames=0044 {ECO:0000303|PubMed:18554329},
GN   D806_000420 {ECO:0000312|EMBL:AWT51036.1}, D806_0045;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155, and MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: May be involved in assembly of the ESX-1 / type VII
CC       specialized secretion system (T7SS), which exports several proteins
CC       including EsxA and EsxB (By similarity). Involved in DNA conjugation in
CC       recipient (MKD8) but not donor (mc(2)155) strain (PubMed:18554329).
CC       {ECO:0000250|UniProtKB:A0QNH4, ECO:0000269|PubMed:18554329}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain (MKD8), no effect when disrupted in donor strain
CC       (mc(2)155) (PubMed:18554329). The recipient strain does not secrete
CC       EsxB (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
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DR   EMBL; CP027541; AWT51036.1; -; Genomic_DNA.
DR   RefSeq; WP_003891372.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FM21; -.
DR   EnsemblBacteria; AWT51036; AWT51036; D806_000420.
DR   PATRIC; fig|1214915.3.peg.45; -.
DR   HOGENOM; CLU_397843_0_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
PE   3: Inferred from homology;
FT   CHAIN           1..692
FT                   /note="Putative ESX-1 scaffolding and assembly protein
FT                   SaeA"
FT                   /id="PRO_0000438352"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          87..134
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        88..104
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   692 AA;  74904 MW;  88008794B83EF9F2 CRC64;
     MGERGELVSD LHPSDDHDAD PRLAPLLAWR QQLVDSGAVA PRSFKEAHLR LVLRSGRTDV
     EQIRAMLPGS VAEHAEEMAR ILAELTPAAP EPDPPPVPEP QPEPEPGPGK HRSPETDEPP
     VATTTEIPIP TTGFAPFQFS SQQVALHDIT VQRTDAAVEL SWPPYEAPDD EAARDISVVM
     YRVVSSDDQA PYSPDPAHLV ALTEEPKATD ERQQVSPVRH YQVWVNVGAS EAAARKAQPV
     LYATAVLVRP VTGFEIREDA GWVIGQWTAP PGVTAVHVFR VPIDEVDRDE AQYRILTAGE
     NLAGFVDTEP VRGQRYRYRA RCAVNVDGVV RLSEAAEADV ELAAALMPVT DLVVETAADG
     ASCDLSWTPP AGGQVAIYRS QNGPSAGAEA IELPQGALEQ VGLTPELRVT QDLTEETGSD
     GRRRARLTGV TWPSEWSRAY FTPVTLMGER AMLGRTLSSV RTGTIRDIEL AEYCNKQVLT
     FDWPDGAASV IVYLAPKGHD PRSGLNGRSF EISLEEYERY GGMHLTGQLP VGGCSLHLAP
     VAFAGGRRVV GAFSSIEYRG LLRLQYAVRI GRDPNGFPTT ATIALRAEQN VPGSPGFVLV
     NNPQRLPLSV HDGHPVDVAP LDARGQLADH PSKELRWSAL TTSGDGELWA ANLSGLQGWI
     RLFVNIGSPA QLRVIALLDP PVETLRLTAA TL