SAEB_MYCS2
ID SAEB_MYCS2 Reviewed; 822 AA.
AC A0QNH5; I7G2D7;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Putative ESX-1 scaffolding and assembly protein SaeB;
GN Name=saeB {ECO:0000303|PubMed:22233444};
GN OrderedLocusNames=MSMEG_0045, MSMEI_0047;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155, and MKD8;
RX PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT Mycobacterium smegmatis.";
RL Mol. Microbiol. 69:794-808(2008).
RN [5]
RP PUTATIVE FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA Gray T.A., Derbyshire K.M.;
RT "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT secretory apparatus in mycobacteria.";
RL Mol. Microbiol. 83:654-664(2012).
CC -!- FUNCTION: May be involved in assembly of the ESX-1 / type VII
CC specialized secretion system (T7SS), which exports several proteins
CC including EsxA and EsxB (PubMed:22233444). Involved in DNA conjugation
CC in recipient (MDK8) but not donor (mc(2)155) strain (PubMed:18554329).
CC {ECO:0000269|PubMed:18554329, ECO:0000305|PubMed:22233444}.
CC -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC recipient strain, no effect when disrupted in donor strain
CC (PubMed:18554329). The recipient strain secretes reduced amounts of
CC EsxB (PubMed:18554329). Disruption of the probable saeA-saeB-saeC
CC operon completely blocks polar localization of EccCb1
CC (PubMed:22233444). {ECO:0000269|PubMed:18554329,
CC ECO:0000269|PubMed:22233444}.
CC -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC distinct donor and recipient strains; mc(2)155 is a donor strain while
CC MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC transfer do not always alter DNA transfer in a recipient strain.
CC {ECO:0000305|PubMed:18554329}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFP36529.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK71610.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36529.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_011726629.1; NZ_SIJM01000001.1.
DR RefSeq; YP_884463.1; NC_008596.1.
DR AlphaFoldDB; A0QNH5; -.
DR STRING; 246196.MSMEI_0047; -.
DR EnsemblBacteria; ABK71610; ABK71610; MSMEG_0045.
DR EnsemblBacteria; AFP36529; AFP36529; MSMEI_0047.
DR GeneID; 66738235; -.
DR KEGG; msg:MSMEI_0047; -.
DR KEGG; msm:MSMEG_0045; -.
DR PATRIC; fig|246196.19.peg.43; -.
DR eggNOG; ENOG502ZI73; Bacteria.
DR OMA; WCELVGA; -.
DR OrthoDB; 130370at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR InterPro; IPR045401; GAP1-M.
DR InterPro; IPR045402; GAP1-N2.
DR Pfam; PF20014; GAP1-M; 1.
DR Pfam; PF20013; GAP1-N2; 1.
PE 4: Predicted;
KW Reference proteome.
FT CHAIN 1..822
FT /note="Putative ESX-1 scaffolding and assembly protein
FT SaeB"
FT /id="PRO_0000438353"
SQ SEQUENCE 822 AA; 86601 MW; 4954801A877F551B CRC64;
MTSRFGQLTY TSFDAVGTVG GWQVKETSDA LTPEETQLML AGVRTVFRTV EPMPDYPTPE
QLEAAPRRLA YSRVGETYAA LWHTVPAGAD STGRPGNVFA HVLLDRTPDV APRHRAIQWW
RSPHWLCPYG ATAVSRAALA EPGAEPGRVV TKDSVVAFAL DTTTWRLATL FGLLDAVAAA
LDGGPPVVLG VESPDNAAQW IGLLSFLMSP GTAAQLSFST FDRADQLNPH SGQMLSAVPL
EDLSAVPAGM VAISEAETLS LGELGGEPHR TAGGYAIDVT PWSAMAQVVV LDPGSARRLL
DDIDAVAEQV RDSGLHPAWP MAMAVAGRAE FADAEEEAHE VIAAHSPPGV AVGSAAARTI
SGVLSAAVGT TTADAWRAVQ ELPTGPGAVF ADTTYLCRAV TDDEWLSENS PIPLGPRMFH
GKPIPPPLRT AIGRALDPGR GPQRLLQVAD LLVRAGVEDP RIRTTLVEDV VPRLGDAEVR
ERVGADARLT LGAVLLNDGD ANGTAIDDGL LDWLADTAPL PPPDELAQAT PWDRTWTIAA
LRGVRARRRG TGGAGQDAGA LLWWLRATGS ADFEQTATAS AWNPEDLLLA IGADPLPGAA
AVRTLVAAAD SPALNRLAAK VIDENGDTLA VACAAVRTIE PTVWLQQRYV ETHQHAYVPL
WDEVLSVVDP ADVHPDFSTR LLAFALLGLF TGHPYPRACS GFAASDRLGG EAIERLMPLV
GEGQLAPHAV VAIGLLRAAA PPDPAHPDVT LDELAGHLAE QVATGMAGDD NDVDGVVAVM
AQLSGDSAEP ALRGYRKMVT KLLARRGDSP SLTARLRGGR QA
