ID   SAEB_MYCSE              Reviewed;         822 AA.
AC   L8FL04; A0A2U9PH40;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Putative ESX-1 scaffolding and assembly protein SaeB;
GN   Name=saeB;
GN   ORFNames=0044 {ECO:0000303|PubMed:18554329},
GN   D806_000430 {ECO:0000312|EMBL:AWT51037.1}, D806_0046;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155, and MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: May be involved in assembly of the ESX-1 / type VII
CC       specialized secretion system (T7SS), which exports several proteins
CC       including EsxA and EsxB (By similarity). Involved in DNA conjugation in
CC       recipient (MKD8) but not donor (mc(2)155) strain (PubMed:18554329).
CC       {ECO:0000250|UniProtKB:A0QNH5, ECO:0000269|PubMed:18554329}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, no effect when disrupted in donor strain
CC       (PubMed:18554329). The recipient strain secretes reduced amounts of
CC       EsxB (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
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DR   EMBL; CP027541; AWT51037.1; -; Genomic_DNA.
DR   RefSeq; WP_003891373.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FL04; -.
DR   EnsemblBacteria; AWT51037; AWT51037; D806_000430.
DR   PATRIC; fig|1214915.3.peg.46; -.
DR   HOGENOM; CLU_345074_0_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   InterPro; IPR045401; GAP1-M.
DR   InterPro; IPR045402; GAP1-N2.
DR   Pfam; PF20014; GAP1-M; 1.
DR   Pfam; PF20013; GAP1-N2; 1.
PE   3: Inferred from homology;
FT   CHAIN           1..822
FT                   /note="Putative ESX-1 scaffolding and assembly protein
FT                   SaeB"
FT                   /id="PRO_0000438354"
SQ   SEQUENCE   822 AA;  86625 MW;  BCF40862FE5CF07C CRC64;
     MTSRFGQLTY TSFDAVGTVG GWQVKETTDA LTSEETQLML AGVRTVFRTV EPMPDYPTPE
     QLEAAPRRLA YSRVGETYAA LWHTVPAGAD STGRPGNVFA HVLLDRTPDV APRHRAIQWW
     RSPHWLCPYG ATSVSRAALA EPGPEPGGVV TKDSVVAFAL DTTTWRLATL FGLLDAVAAA
     LDGGAPVVLG VESPDNAAQW IGLLSFLMSP GTAAQLSFST FDRADQLNLH GGQVLSAVPL
     EDLSAVPAGM AAISEAETLS LGELGGEPHR TAGGYAIDVT PWSAMAQVVL LDPGSARRLL
     DDIDAVAEQV RDSGLHPAWP MAMAVAGRAE FADAEEEAHE VIAAHSPPGV AVGSAAARTI
     SGVLSAVVGT TTADAWRAVQ ELPTGPGAVF ADTTYLCRAV ADDAWLSNSG PIPLGPRMFH
     GKPIPPPLRT AIGDALNPAR GPERLLQVAD LLVRAGVEDP RIRTTLVEDV VPRLRDAEAR
     ERVGADARLT LGAVLLNDGD ANGTAIDDGL LDWLADTAPL PPPDELAQAT PWDRTWTTAA
     LRGVRAWRRG TGGAGRDAGA LLWWLRMAGS ADFEQTATAS AWNPEDLLLA IGADPLPGAA
     AVRTLVAAAD SPALNRLAAK VIDENGDSLA VACAAVRTIE PTVWLQQRYV ETHQHAYVPL
     WDEVLSVVDP ADVHPDFSTR LLAFALLGLF TGHPYPRACS GFAASDRLGG EAIERLMPLV
     GEGHLAPHAV VAISLLRAAA PPDPSHPDVT LDELAGHLAE QVATGMVGDD NDVEGVVAVM
     AQLSGDSAEP ALRGYRKMVT KLMARRRDSP SLTARLRGGM QA