ID   SAEC_MYCS2              Reviewed;         385 AA.
AC   A0QNH6;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Putative ESX-1 scaffolding and assembly protein SaeC;
GN   Name=saeC {ECO:0000303|PubMed:22233444};
GN   OrderedLocusNames=MSMEG_0046, MSMEI_0048;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA   Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA   Gray T.A., Derbyshire K.M.;
RT   "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT   secretory apparatus in mycobacteria.";
RL   Mol. Microbiol. 83:654-664(2012).
CC   -!- FUNCTION: May be involved in assembly of the ESX-1 / type VII
CC       specialized secretion system (T7SS), which exports several proteins
CC       including EsxA and EsxB (PubMed:22233444). Involved in DNA conjugation
CC       in recipient (MKD8) strain (PubMed:22233444).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22233444}.
CC       Note=Localizes at or near the cell pole (usually the old or non-septal
CC       cell pole) in (on average) 1 discrete spot that also colocalizes with
CC       EspE and EccCb1 (PubMed:22233444). Localization occurs in the absence
CC       of the ESX-1 locus (PubMed:22233444). {ECO:0000269|PubMed:22233444}.
CC   -!- DISRUPTION PHENOTYPE: Secretes greatly reduced amounts of EsxB, protein
CC       is synthesized intracellularly (PubMed:22233444). In single deletion
CC       mutant EccCb1 only rarely localizes near the cell pole, while
CC       disruption of the probable saeA-saeB-saeC operon completely blocks
CC       polar localization of EccCb1 (PubMed:22233444). Loss of DNA conjugation
CC       when disrupted in recipient strain (MKD8) (PubMed:22233444).
CC       {ECO:0000269|PubMed:22233444}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:22233444}.
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DR   EMBL; CP000480; ABK70450.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36530.1; -; Genomic_DNA.
DR   RefSeq; WP_003891374.1; NZ_SIJM01000001.1.
DR   RefSeq; YP_884464.1; NC_008596.1.
DR   AlphaFoldDB; A0QNH6; -.
DR   STRING; 246196.MSMEI_0048; -.
DR   PRIDE; A0QNH6; -.
DR   EnsemblBacteria; ABK70450; ABK70450; MSMEG_0046.
DR   EnsemblBacteria; AFP36530; AFP36530; MSMEI_0048.
DR   GeneID; 66738236; -.
DR   KEGG; msg:MSMEI_0048; -.
DR   KEGG; msm:MSMEG_0046; -.
DR   PATRIC; fig|246196.19.peg.44; -.
DR   eggNOG; COG1100; Bacteria.
DR   OMA; QEPFIFQ; -.
DR   OrthoDB; 1052728at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR045528; DO-GTPase2.
DR   Pfam; PF19993; DO-GTPase2; 1.
PE   4: Predicted;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..385
FT                   /note="Putative ESX-1 scaffolding and assembly protein
FT                   SaeC"
FT                   /id="PRO_0000438355"
SQ   SEQUENCE   385 AA;  42111 MW;  E74E81A2B18BFC84 CRC64;
     MSKCPRCFTA LNPNQYLWTL PEQSGGTRYR DEVATAYSGA PADCGPLYTW SRSPGYNGPP
     PPTSEATRAL QAPAVEICPV CHFTLPEGWR EGHAICIALA GARATGKSLY IAVLVKQLEL
     LCERFGVSMQ PVTRGTAQAY ATNYETPLYV QRGLIPPTPT VRTQTSDHRE PLMFSLGIWH
     GVRRFLVLRD VAGEDMESGD LHAPPFRFFA HADAVFFMFD PLRVKTIRDQ LHDLLPAQVV
     SGGDPRTVLT NVLTAVSPGQ PKLAVILSKF DVLRALRDVE GSEWGLIMSN GGAAYLRDTS
     DGQQYDETDG QLLHEEVRSL LLRLHGGSIL AAVENPATGV RLQSRNFVVS ALGHPPSGNR
     LHARGIAPFR CVDPIRWVTS GFGVL