ID   SAFA_ECO57              Reviewed;          65 AA.
AC   Q8XAX0; Q7AE10;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Two-component-system connector protein SafA;
GN   Name=safA; OrderedLocusNames=Z2208, ECs2105;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Connects the signal transduction between the two-component
CC       systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and
CC       thus activating the PhoQ/PhoP system, in response to acid stress
CC       conditions. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PhoQ. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       type II membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By acid stress, via the EvgS/EvgA system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SafA family. {ECO:0000305}.
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DR   EMBL; BA000007; BAB35528.1; -; Genomic_DNA.
DR   EMBL; AE005174; AAG56268.1; -; Genomic_DNA.
DR   PIR; A90892; A90892.
DR   PIR; H85725; H85725.
DR   RefSeq; NP_310132.1; NC_002695.1.
DR   RefSeq; WP_000543386.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8XAX0; -.
DR   SMR; Q8XAX0; -.
DR   STRING; 155864.EDL933_2139; -.
DR   EnsemblBacteria; AAG56268; AAG56268; Z2208.
DR   EnsemblBacteria; BAB35528; BAB35528; ECs_2105.
DR   GeneID; 917320; -.
DR   KEGG; ece:Z2208; -.
DR   KEGG; ecs:ECs_2105; -.
DR   PATRIC; fig|386585.9.peg.2211; -.
DR   HOGENOM; CLU_2842804_0_0_6; -.
DR   OMA; NYKGIMS; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031411; SafA.
DR   Pfam; PF17073; SafA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Signal-anchor; Stress response; Transmembrane; Transmembrane helix.
FT   CHAIN           1..65
FT                   /note="Two-component-system connector protein SafA"
FT                   /id="PRO_0000223716"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..65
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   65 AA;  7328 MW;  EA9E42AA4C9737ED CRC64;
     MHATTVKNKI TQRDNYKEIM SVIVVILLLT LTLIAIFSAI DQLGISEMGR IARDLTHFIV
     NSLQD