SAFA_ECOLI
ID SAFA_ECOLI Reviewed; 65 AA.
AC P76136; B2D6K4;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Two-component-system connector protein SafA;
DE AltName: Full=Regulatory protein b1500;
DE AltName: Full=Sensor associating factor A;
GN Name=safA; Synonyms=yneN; OrderedLocusNames=b1500, JW1494.1;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP INDUCTION BY ACID STRESS.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12694615; DOI=10.1046/j.1365-2958.2003.03477.x;
RA Masuda N., Church G.M.;
RT "Regulatory network of acid resistance genes in Escherichia coli.";
RL Mol. Microbiol. 48:699-712(2003).
RN [4]
RP FUNCTION IN RESPONSE TO ACID STRESS, SUBCELLULAR LOCATION, TOPOLOGY, AND
RP INTERACTION WITH PHOQ.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=17998538; DOI=10.1073/pnas.0705768104;
RA Eguchi Y., Itou J., Yamane M., Demizu R., Yamato F., Okada A., Mori H.,
RA Kato A., Utsumi R.;
RT "B1500, a small membrane protein, connects the two-component systems
RT EvgS/EvgA and PhoQ/PhoP in Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:18712-18717(2007).
RN [5]
RP INDUCTION, AND GENE NAME.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19352034; DOI=10.1271/bbb.80795;
RA Itou J., Eguchi Y., Utsumi R.;
RT "Molecular mechanism of transcriptional cascade initiated by the EvgS/EvgA
RT system in Escherichia coli K-12.";
RL Biosci. Biotechnol. Biochem. 73:870-878(2009).
CC -!- FUNCTION: Connects the signal transduction between the two-component
CC systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and
CC thus activating the PhoQ/PhoP system, in response to acid stress
CC conditions. {ECO:0000269|PubMed:17998538}.
CC -!- SUBUNIT: Interacts with PhoQ. {ECO:0000269|PubMed:17998538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:17998538}; Single-pass type II membrane protein
CC {ECO:0000269|PubMed:17998538}.
CC -!- INDUCTION: By acid stress, via the EvgS/EvgA system.
CC {ECO:0000269|PubMed:12694615, ECO:0000269|PubMed:19352034}.
CC -!- SIMILARITY: Belongs to the SafA family. {ECO:0000305}.
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DR EMBL; U00096; AAC74573.1; -; Genomic_DNA.
DR EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; G64903; G64903.
DR RefSeq; NP_416017.1; NC_000913.3.
DR RefSeq; WP_000543384.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P76136; -.
DR SMR; P76136; -.
DR DIP; DIP-46181N; -.
DR IntAct; P76136; 1.
DR STRING; 511145.b1500; -.
DR PaxDb; P76136; -.
DR PRIDE; P76136; -.
DR EnsemblBacteria; AAC74573; AAC74573; b1500.
DR GeneID; 946061; -.
DR KEGG; eco:b1500; -.
DR PATRIC; fig|1411691.4.peg.766; -.
DR OMA; NYKGIMS; -.
DR BioCyc; EcoCyc:MON0-2841; -.
DR PRO; PR:P76136; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0010447; P:response to acidic pH; IEP:EcoCyc.
DR InterPro; IPR031411; SafA.
DR Pfam; PF17073; SafA; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Signal-anchor; Stress response; Transmembrane; Transmembrane helix.
FT CHAIN 1..65
FT /note="Two-component-system connector protein SafA"
FT /id="PRO_0000223715"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:17998538"
FT TRANSMEM 19..39
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..65
FT /note="Periplasmic"
FT /evidence="ECO:0000269|PubMed:17998538"
SQ SEQUENCE 65 AA; 7272 MW; 10ECD1B9EE15463E CRC64;
MHATTVKNKI TQRDNYKEIM SAIVVVLLLT LTLIAIFSAI DQLSISEMGR IARDLTHFII
NSLQG
