ID   SAFA_ECOSM              Reviewed;          65 AA.
AC   B1LFB6;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Two-component-system connector protein SafA;
GN   Name=safA; OrderedLocusNames=EcSMS35_1673;
OS   Escherichia coli (strain SMS-3-5 / SECEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=439855;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SMS-3-5 / SECEC;
RX   PubMed=18708504; DOI=10.1128/jb.00661-08;
RA   Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C.,
RA   Ravel J., Stepanauskas R.;
RT   "Insights into the environmental resistance gene pool from the genome
RT   sequence of the multidrug-resistant environmental isolate Escherichia coli
RT   SMS-3-5.";
RL   J. Bacteriol. 190:6779-6794(2008).
CC   -!- FUNCTION: Connects the signal transduction between the two-component
CC       systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and
CC       thus activating the PhoQ/PhoP system, in response to acid stress
CC       conditions. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PhoQ. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       type II membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By acid stress, via the EvgS/EvgA system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SafA family. {ECO:0000305}.
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DR   EMBL; CP000970; ACB16698.1; -; Genomic_DNA.
DR   RefSeq; WP_000543380.1; NC_010498.1.
DR   AlphaFoldDB; B1LFB6; -.
DR   SMR; B1LFB6; -.
DR   EnsemblBacteria; ACB16698; ACB16698; EcSMS35_1673.
DR   KEGG; ecm:EcSMS35_1673; -.
DR   HOGENOM; CLU_2842804_0_0_6; -.
DR   OMA; NYKGIMS; -.
DR   Proteomes; UP000007011; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031411; SafA.
DR   Pfam; PF17073; SafA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Signal-anchor;
KW   Stress response; Transmembrane; Transmembrane helix.
FT   CHAIN           1..65
FT                   /note="Two-component-system connector protein SafA"
FT                   /id="PRO_0000340117"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..65
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   65 AA;  7391 MW;  CF25C8AA4C85A4AD CRC64;
     MHATTVKNKI MQRDNYKEIM SVIVVVLLLT LTLIAIFSAI DQQGISEMGR MARDLTHFII
     NSLQD