ID   SAFA_SHIDS              Reviewed;          65 AA.
AC   Q32FX5;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Two-component-system connector protein SafA;
GN   Name=safA; OrderedLocusNames=SDY_1659;
OS   Shigella dysenteriae serotype 1 (strain Sd197).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=300267;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sd197;
RX   PubMed=16275786; DOI=10.1093/nar/gki954;
RA   Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J.,
RA   Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J.,
RA   Xu J., Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J.,
RA   Jin Q.;
RT   "Genome dynamics and diversity of Shigella species, the etiologic agents of
RT   bacillary dysentery.";
RL   Nucleic Acids Res. 33:6445-6458(2005).
CC   -!- FUNCTION: Connects the signal transduction between the two-component
CC       systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and
CC       thus activating the PhoQ/PhoP system, in response to acid stress
CC       conditions. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PhoQ. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       type II membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By acid stress, via the EvgS/EvgA system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SafA family. {ECO:0000305}.
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DR   EMBL; CP000034; ABB61780.1; -; Genomic_DNA.
DR   RefSeq; WP_000543400.1; NC_007606.1.
DR   RefSeq; YP_403271.1; NC_007606.1.
DR   AlphaFoldDB; Q32FX5; -.
DR   SMR; Q32FX5; -.
DR   EnsemblBacteria; ABB61780; ABB61780; SDY_1659.
DR   KEGG; sdy:SDY_1659; -.
DR   HOGENOM; CLU_2842804_0_0_6; -.
DR   OMA; NYKGIMS; -.
DR   Proteomes; UP000002716; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031411; SafA.
DR   Pfam; PF17073; SafA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Signal-anchor; Stress response; Transmembrane; Transmembrane helix.
FT   CHAIN           1..65
FT                   /note="Two-component-system connector protein SafA"
FT                   /id="PRO_0000223719"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..65
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   65 AA;  7256 MW;  FF8A094A4C85A7FC CRC64;
     MHATTVKNKI TQRDNYKGIM SVIVVVLLLT LTLIAIFSAI DQLGISEMGR IARDLTHFII
     NSLQD