ID   SAFA_SHIFL              Reviewed;          67 AA.
AC   Q7UCH6;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Two-component-system connector protein SafA;
GN   Name=safA; OrderedLocusNames=SF1537.1, S1655;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Connects the signal transduction between the two-component
CC       systems EvgS/EvgA and PhoQ/PhoP, by directly interacting with PhoQ and
CC       thus activating the PhoQ/PhoP system, in response to acid stress
CC       conditions. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PhoQ. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       type II membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By acid stress, via the EvgS/EvgA system. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SafA family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005674; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AE014073; AAP17018.1; -; Genomic_DNA.
DR   RefSeq; WP_000543395.1; NZ_WPGV01000167.1.
DR   AlphaFoldDB; Q7UCH6; -.
DR   SMR; Q7UCH6; -.
DR   EnsemblBacteria; AAP17018; AAP17018; S1655.
DR   KEGG; sft:NCTC1_01659; -.
DR   KEGG; sfx:S1655; -.
DR   PATRIC; fig|623.158.peg.1684; -.
DR   HOGENOM; CLU_2842804_0_0_6; -.
DR   OMA; NYKGIMS; -.
DR   OrthoDB; 2034505at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031411; SafA.
DR   Pfam; PF17073; SafA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Signal-anchor; Stress response; Transmembrane; Transmembrane helix.
FT   CHAIN           1..67
FT                   /note="Two-component-system connector protein SafA"
FT                   /id="PRO_0000223720"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..65
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   67 AA;  7672 MW;  28F6223CD1AA4C9F CRC64;
     MHATTVKNKI TQRDNYKEIM SVIVVVLLLT LTLIAIFSAI DQLSISEMGR IARDLTHFII
     NSLQDWK