ID   SAG39_ORYSI             Reviewed;         339 AA.
AC   A2XQE8;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Senescence-specific cysteine protease SAG39 {ECO:0000305};
DE            EC=3.4.22.- {ECO:0000305};
DE   AltName: Full=Cysteine proteinase SAG39 {ECO:0000305};
DE   AltName: Full=Protein SENESCENCE-ASSOCIATED GENE 39 {ECO:0000250|UniProtKB:Q7XWK5};
DE   Flags: Precursor;
GN   ORFNames=OsI_14861 {ECO:0000312|EMBL:EAY93058.1};
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946 {ECO:0000312|EMBL:EAY93058.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Cysteine protease that may have a developmental senescence
CC       specific cell death function during apoptosis, heavy metal
CC       detoxification, and hypersensitive response.
CC       {ECO:0000250|UniProtKB:Q9FJ47}.
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250|UniProtKB:Q9FJ47}.
CC       Note=Localized in senescence-associated vacuoles (SAVs) with intense
CC       proteolytic activity that develop in the peripheral cytoplasm of
CC       mesophyll and guard cells. {ECO:0000250|UniProtKB:Q9FJ47}.
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC       ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR   EMBL; CM000129; EAY93058.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2XQE8; -.
DR   SMR; A2XQE8; -.
DR   STRING; 39946.A2XQE8; -.
DR   MEROPS; C01.104; -.
DR   EnsemblPlants; BGIOSGA015901-TA; BGIOSGA015901-PA; BGIOSGA015901.
DR   Gramene; BGIOSGA015901-TA; BGIOSGA015901-PA; BGIOSGA015901.
DR   HOGENOM; CLU_012184_1_0_1; -.
DR   OMA; FRSTKTN; -.
DR   Proteomes; UP000007015; Chromosome 4.
DR   GO; GO:0010282; C:senescence-associated vacuole; ISS:UniProtKB.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0007568; P:aging; ISS:UniProtKB.
DR   GO; GO:0010150; P:leaf senescence; IEA:EnsemblPlants.
DR   GO; GO:0010623; P:programmed cell death involved in cell development; ISS:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0009737; P:response to abscisic acid; IEA:EnsemblPlants.
DR   GO; GO:0009723; P:response to ethylene; IEA:EnsemblPlants.
DR   GO; GO:0009739; P:response to gibberellin; IEA:EnsemblPlants.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR025661; Pept_asp_AS.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR025660; Pept_his_AS.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   InterPro; IPR013201; Prot_inhib_I29.
DR   Pfam; PF08246; Inhibitor_I29; 1.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   PRINTS; PR00705; PAPAIN.
DR   SMART; SM00848; Inhibitor_I29; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR   PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Signal; Thiol protease; Vacuole.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..339
FT                   /note="Senescence-specific cysteine protease SAG39"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000430526"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT   ACT_SITE        282
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10089"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10090"
SQ   SEQUENCE   339 AA;  36842 MW;  E36D71DBE01A0A4B CRC64;
     MAMAKALLFA ILGCLCLCSA VLAARELSDD AAMAARHERW MAQYGRVYRD DAEKARRFEV
     FKANVAFIES FNAGNHNFWL GVNQFADLTN DEFRWTKTNK GFIPSTTRVP TGFRYENVNI
     DALPATVDWR TKGAVTPIKD QGQCGCCWAF SAVAAMEGIV KLSTGKLISL SEQELVDCDV
     HGEDQGCEGG LMDDAFKFII KNGGLTTESN YPYAAADDKC KSVSNSVASI KGYEDVPANN
     EAALMKAVAN QPVSVAVDGG DMTFQFYKGG VMTGSCGTDL DHGIVAIGYG KASDGTKYWL
     LKNSWGTTWG ENGFLRMEKD ISDKRGMCGL AMEPSYPTA