ID   SAHS2_RAMVA             Reviewed;         174 AA.
AC   J7MAN2;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Secretory-abundant heat soluble protein 2 {ECO:0000303|PubMed:22937162};
DE            Short=SAHS2 {ECO:0000303|PubMed:22937162};
DE   AltName: Full=Tardigrade-specific intrinsically disordered protein SAHS2 {ECO:0000305};
DE            Short=TDP SAHS2 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=SAHS2 {ECO:0000303|PubMed:22937162}; ORFNames=RvY_02431;
OS   Ramazzottius varieornatus (Water bear) (Tardigrade).
OC   Eukaryota; Metazoa; Ecdysozoa; Tardigrada; Eutardigrada; Parachela;
OC   Hypsibioidea; Ramazzottiidae; Ramazzottius.
OX   NCBI_TaxID=947166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION,
RP   AND DOMAIN.
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=22937162; DOI=10.1371/journal.pone.0044209;
RA   Yamaguchi A., Tanaka S., Yamaguchi S., Kuwahara H., Takamura C.,
RA   Imajoh-Ohmi S., Horikawa D.D., Toyoda A., Katayama T., Arakawa K.,
RA   Fujiyama A., Kubo T., Kunieda T.;
RT   "Two novel heat-soluble protein families abundantly expressed in an
RT   anhydrobiotic tardigrade.";
RL   PLoS ONE 7:E44209-E44209(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=27649274; DOI=10.1038/ncomms12808;
RA   Hashimoto T., Horikawa D.D., Saito Y., Kuwahara H., Kozuka-Hata H.,
RA   Shin-I T., Minakuchi Y., Ohishi K., Motoyama A., Aizu T., Enomoto A.,
RA   Kondo K., Tanaka S., Hara Y., Koshikawa S., Sagara H., Miura T.,
RA   Yokobori S., Miyagawa K., Suzuki Y., Kubo T., Oyama M., Kohara Y.,
RA   Fujiyama A., Arakawa K., Katayama T., Toyoda A., Kunieda T.;
RT   "Extremotolerant tardigrade genome and improved radiotolerance of human
RT   cultured cells by tardigrade-unique protein.";
RL   Nat. Commun. 7:12808-12808(2016).
CC   -!- FUNCTION: Secreted heat soluble protein acting as a molecular shield in
CC       water-deficient condition (PubMed:22937162). Tardigrade-specific
CC       intrinsically disordered proteins (TDPs) are essential for desiccation
CC       tolerance by forming non-crystalline amorphous solids upon desiccation,
CC       and this vitrified state mirrors their protective capabilities (By
CC       similarity). {ECO:0000250|UniProtKB:J7MFT5}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:J7MFT5}.
CC   -!- DOMAIN: SAHS-c1, SAHS-c2 and SAHS-c3 are 3 highly conserved regions
CC       within the SAHS protein family (PubMed:22937162).
CC       {ECO:0000305|PubMed:22937162}.
CC   -!- MISCELLANEOUS: Trehalose, a disaccharide essential for several
CC       organisms to survive drying, is detected at low levels or not at all in
CC       some tardigrade species, indicating that tardigrades possess
CC       potentially novel mechanisms for surviving desiccation involving
CC       tardigrade-specific intrinsically disordered proteins (TDPs) (By
CC       similarity). {ECO:0000250|UniProtKB:P0CU39}.
CC   -!- SIMILARITY: Belongs to the Secretory-abundant heat soluble protein
CC       (SAHS) family. {ECO:0000305}.
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DR   EMBL; AB650498; BAM37957.1; -; mRNA.
DR   EMBL; BDGG01000001; GAU89940.1; -; Genomic_DNA.
DR   AlphaFoldDB; J7MAN2; -.
DR   SMR; J7MAN2; -.
DR   Proteomes; UP000186922; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.128.20; -; 1.
DR   InterPro; IPR012674; Calycin.
DR   SUPFAM; SSF50814; SSF50814; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Reference proteome; Secreted; Signal; Stress response.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..174
FT                   /note="Secretory-abundant heat soluble protein 2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5003794980"
FT   REGION          30..60
FT                   /note="SAHS-c1"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   REGION          77..105
FT                   /note="SAHS-c2"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   REGION          118..172
FT                   /note="SAHS-c3"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   CARBOHYD        111
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   174 AA;  19878 MW;  BC02F191D56E4E71 CRC64;
     MHRFVLALVV FAGAAIVWAA DDAAHEEGVE WTGKPWMGKW ESDPSKDENV EEFKKKLQLP
     MSHSEMNKNS KVWIHHYKKG DEYHHKIIIN DAHYKNDIVF KLGQESAGSY NGSSFSVKYE
     DKDGALVGSV HYTGTKEQSL DKTINNVFKL EGDHLVKTST IEGVTMKRHY NKRQ