ID   SAKA_LATSK              Reviewed;          59 AA.
AC   P0A310; P35619; P80097;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Bacteriocin sakacin-A;
DE   Flags: Precursor;
GN   Name=sapA; Synonyms=sakA;
OS   Latilactobacillus sakei (Lactobacillus sakei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=1599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE.
RC   STRAIN=Lb706;
RX   PubMed=1487735; DOI=10.1099/00221287-138-12-2715;
RA   Holck A.L., Axelsson L., Birkeland S.-E., Aukrust T., Blom H.;
RT   "Purification and amino acid sequence of sakacin A, a bacteriocin from
RT   Lactobacillus sake Lb706.";
RL   J. Gen. Microbiol. 138:2715-2720(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Lb706;
RX   PubMed=7721704; DOI=10.1128/jb.177.8.2125-2137.1995;
RA   Axelsson L., Holck A.;
RT   "The genes involved in production of and immunity to sakacin A, a
RT   bacteriocin from Lactobacillus sake Lb706.";
RL   J. Bacteriol. 177:2125-2137(1995).
CC   -!- FUNCTION: Bactericidal activity; inhibits closely related Lactobacilli,
CC       Listeria monocytogenes and ivanovvi, Enterococcus faecalis,
CC       Carnobacterium sp and Brocothrix thermosphacta.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z14233; CAA78600.1; -; Genomic_DNA.
DR   EMBL; Z46867; CAA86942.1; -; Genomic_DNA.
DR   PIR; C56273; C56273.
DR   RefSeq; WP_032488606.1; NZ_MKGH01000063.1.
DR   AlphaFoldDB; P0A310; -.
DR   SMR; P0A310; -.
DR   TCDB; 1.C.24.1.8; the pediocin (pediocin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.5.130; -; 1.
DR   InterPro; IPR002633; Bacteriocin_IIa.
DR   InterPro; IPR023384; Bacteriocin_IIa_CS.
DR   InterPro; IPR023388; Bacteriocin_IIa_dom_sf.
DR   InterPro; IPR010133; Bacteriocin_signal_seq.
DR   Pfam; PF01721; Bacteriocin_II; 1.
DR   TIGRFAMs; TIGR01847; bacteriocin_sig; 1.
DR   PROSITE; PS60030; BACTERIOCIN_IIA; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Disulfide bond; Secreted.
FT   PROPEP          1..18
FT                   /id="PRO_0000002749"
FT   CHAIN           19..59
FT                   /note="Bacteriocin sakacin-A"
FT                   /id="PRO_0000002750"
FT   DISULFID        28..33
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   59 AA;  6257 MW;  CEE4B0EB5470574A CRC64;
     MNNVKELSMT ELQTITGGAR SYGNGVYCNN KKCWVNRGEA TQSIIGGMIS GWASGLAGM