ID   SAKP_LATSK              Reviewed;          61 AA.
AC   P35618; Q57121;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Bacteriocin sakacin-P;
DE   AltName: Full=Sakacin 674;
DE   Flags: Precursor;
GN   Name=sakP; Synonyms=sakR, sppA;
OS   Latilactobacillus sakei (Lactobacillus sakei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=1599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LTH673;
RX   PubMed=8180701; DOI=10.1099/13500872-140-2-361;
RA   Tichaczek P.S., Vogel R.F., Hammes W.P.;
RT   "Cloning and sequencing of sakP encoding sakacin P, the bacteriocin
RT   produced by Lactobacillus sake LTH 673.";
RL   Microbiology 140:361-367(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-61, AND
RP   CHARACTERIZATION.
RC   STRAIN=Lb674;
RX   PubMed=8138128; DOI=10.1111/j.1574-6968.1994.tb06629.x;
RA   Holck A.L., Axelsson L., Huehne K., Kroeckel L.;
RT   "Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus
RT   sake Lb674.";
RL   FEMS Microbiol. Lett. 115:143-150(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Lb674;
RX   PubMed=8704983; DOI=10.1099/13500872-142-6-1437;
RA   Huehne K., Axelsson L., Holck A., Kroeckel L.;
RT   "Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and
RT   its expression in sakacin-negative Lb. sake strains.";
RL   Microbiology 142:1437-1448(1996).
RN   [4]
RP   PROTEIN SEQUENCE OF 19-59, AND CHARACTERIZATION.
RC   STRAIN=LTH673;
RA   Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.;
RT   "Characterization of the bacteriocins curvacin A from Lactobacillus
RT   curvatus LTH1174 and sakacin P from L. sake LTH673.";
RL   Syst. Appl. Microbiol. 15:460-465(1992).
RN   [5]
RP   STRUCTURE BY NMR OF 19-61, AND DISULFIDE BOND.
RX   PubMed=14516192; DOI=10.1021/bi034572i;
RA   Uteng M., Hauge H.H., Markwick P.R., Fimland G., Mantzilas D.,
RA   Nissen-Meyer J., Muhle-Goll C.;
RT   "Three-dimensional structure in lipid micelles of the pediocin-like
RT   antimicrobial peptide sakacin P and a sakacin P variant that is
RT   structurally stabilized by an inserted C-terminal disulfide bridge.";
RL   Biochemistry 42:11417-11426(2003).
CC   -!- FUNCTION: Bactericidal activity; inhibits closely related Lactobacilli,
CC       Listeria monocytogenes and ivanovvi, Enterococcus faecalis,
CC       Carnobacterium sp and Brocothrix thermosphacta.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
CC       {ECO:0000305}.
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DR   EMBL; X75081; CAA52974.1; -; Genomic_DNA.
DR   EMBL; Z25816; CAA81064.1; -; Genomic_DNA.
DR   EMBL; Z48542; CAA88428.1; -; Genomic_DNA.
DR   EMBL; AF002276; AAB93970.1; -; Genomic_DNA.
DR   PIR; S57911; S38508.
DR   RefSeq; WP_004271264.1; NZ_LT960790.1.
DR   PDB; 1OG7; NMR; -; A=19-61.
DR   PDB; 1OHM; NMR; -; A=19-61.
DR   PDB; 1OHN; NMR; -; A=19-61.
DR   PDBsum; 1OG7; -.
DR   PDBsum; 1OHM; -.
DR   PDBsum; 1OHN; -.
DR   AlphaFoldDB; P35618; -.
DR   SMR; P35618; -.
DR   TCDB; 1.C.24.1.2; the pediocin (pediocin) family.
DR   EvolutionaryTrace; P35618; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.5.130; -; 1.
DR   InterPro; IPR002633; Bacteriocin_IIa.
DR   InterPro; IPR023384; Bacteriocin_IIa_CS.
DR   InterPro; IPR023388; Bacteriocin_IIa_dom_sf.
DR   Pfam; PF01721; Bacteriocin_II; 1.
DR   PROSITE; PS60030; BACTERIOCIN_IIA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PROPEP          1..18
FT                   /evidence="ECO:0000269|PubMed:8138128, ECO:0000269|Ref.4"
FT                   /id="PRO_0000002751"
FT   CHAIN           19..61
FT                   /note="Bacteriocin sakacin-P"
FT                   /id="PRO_0000002752"
FT   DISULFID        27..32
FT                   /evidence="ECO:0000269|PubMed:14516192"
FT   STRAND          20..22
FT                   /evidence="ECO:0007829|PDB:1OG7"
FT   STRAND          28..30
FT                   /evidence="ECO:0007829|PDB:1OG7"
FT   HELIX           37..50
FT                   /evidence="ECO:0007829|PDB:1OG7"
FT   TURN            53..59
FT                   /evidence="ECO:0007829|PDB:1OG7"
SQ   SEQUENCE   61 AA;  6385 MW;  B7BF14DCDD28A73D CRC64;
     MEKFIELSLK EVTAITGGKY YGNGVHCGKH SCTVDWGTAI GNIGNNAAAN WATGGNAGWN
     K