ID   SAK_BPP42               Reviewed;         163 AA.
AC   P15240;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   23-FEB-2022, entry version 82.
DE   RecName: Full=Staphylokinase;
DE   AltName: Full=Sak42D;
DE   Flags: Precursor;
GN   Name=sak;
OS   Staphylococcus phage 42D (Bacteriophage P42D).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=10715;
OH   NCBI_TaxID=1280; Staphylococcus aureus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3123893; DOI=10.1007/bf00327208;
RA   Behnke D., Gerlach D.;
RT   "Cloning and expression in Escherichia coli, Bacillus subtilis, and
RT   Streptococcus sanguis of a gene for staphylokinase -- a bacterial
RT   plasminogen activator.";
RL   Mol. Gen. Genet. 210:528-534(1987).
CC   -!- FUNCTION: Potent plasminogen activator that converts plasminogen into
CC       plasmin. It forms a 1:1 complex with plasmin, which in turn activates
CC       other plasminogen molecules.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the staphylokinase family. {ECO:0000305}.
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DR   EMBL; X06603; CAA29822.1; -; Genomic_DNA.
DR   EMBL; M57455; AAA98206.1; -; Genomic_DNA.
DR   PIR; S02330; S02330.
DR   PDB; 1BUI; X-ray; 2.65 A; C=38-163.
DR   PDBsum; 1BUI; -.
DR   BMRB; P15240; -.
DR   SMR; P15240; -.
DR   MEROPS; X46.001; -.
DR   EvolutionaryTrace; P15240; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031639; P:plasminogen activation; IEA:InterPro.
DR   InterPro; IPR004093; SAK.
DR   InterPro; IPR036120; SAK/SK_sf.
DR   Pfam; PF02821; Staphylokinase; 1.
DR   SUPFAM; SSF54328; SSF54328; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Plasminogen activation; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..163
FT                   /note="Staphylokinase"
FT                   /id="PRO_0000031605"
FT   STRAND          51..54
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          57..59
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          65..67
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   HELIX           84..98
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          105..107
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          114..120
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   TURN            121..124
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          125..131
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   TURN            144..146
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          151..154
FT                   /evidence="ECO:0007829|PDB:1BUI"
FT   STRAND          157..160
FT                   /evidence="ECO:0007829|PDB:1BUI"
SQ   SEQUENCE   163 AA;  18608 MW;  AA267A4FF75D36EC CRC64;
     MLKRSLLFLT VLLLLFSFSS ITNEVSASSS FDKGKYKKGD DASYFEPTGP YLMVNVTGVD
     GKRNELLSPR YVEFPIKPGT TLTKEKIEYY VEWALDATAY KEFRVVELDP SAKIEVTYYD
     KNKKKEETKS FPITEKGFVV PDLSEHIKNP GFNLITKVVI EKK