ID   SAK_STAAN               Reviewed;         163 AA.
AC   Q99SU7;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Staphylokinase;
DE   Flags: Precursor;
GN   Name=sak; OrderedLocusNames=SA1758;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Potent plasminogen activator that converts plasminogen into
CC       plasmin. It forms a 1:1 complex with plasmin, which in turn activates
CC       other plasminogen molecules (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q99SU7; P00747: PLG; Xeno; NbExp=7; IntAct=EBI-7689378, EBI-999394;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the staphylokinase family. {ECO:0000305}.
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DR   EMBL; BA000018; BAB43032.1; -; Genomic_DNA.
DR   PIR; G89983; G89983.
DR   RefSeq; WP_000920042.1; NC_002745.2.
DR   AlphaFoldDB; Q99SU7; -.
DR   SMR; Q99SU7; -.
DR   IntAct; Q99SU7; 1.
DR   MINT; Q99SU7; -.
DR   EnsemblBacteria; BAB43032; BAB43032; BAB43032.
DR   KEGG; sau:SA1758; -.
DR   HOGENOM; CLU_137975_0_0_9; -.
DR   PRO; PR:Q99SU7; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031639; P:plasminogen activation; IEA:InterPro.
DR   InterPro; IPR004093; SAK.
DR   InterPro; IPR036120; SAK/SK_sf.
DR   Pfam; PF02821; Staphylokinase; 1.
DR   SUPFAM; SSF54328; SSF54328; 1.
PE   1: Evidence at protein level;
KW   Plasminogen activation; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000250"
FT   CHAIN           28..163
FT                   /note="Staphylokinase"
FT                   /id="PRO_0000031600"
SQ   SEQUENCE   163 AA;  18520 MW;  413CDAE14BE9FD40 CRC64;
     MLKRSLLFLT VLLLLFSFSS ITNEVSASSS FDKGKYKKGD DASYFEPTGP YLMVNVTGVD
     SKGNELLSPH YVEFPIKPGT TLTKEKIEYY VEWALDATAY KEFRVVELDP SAKIEVTYYD
     KNKKKEETKS FPITEKGFVV PDLSEHIKNP GFNLITKVVI EKK