SAM10_CAEEL
ID SAM10_CAEEL Reviewed; 455 AA.
AC B7FAS6; B7FAS7;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Single-stranded DNA-binding protein homolog sam-10 {ECO:0000250|UniProtKB:Q9BWW4};
DE AltName: Full=Synaptic vesicle tag abnormal in mechanosensory neurons {ECO:0000303|PubMed:21115607};
GN Name=sam-10 {ECO:0000312|WormBase:Y48C3A.8a};
GN ORFNames=Y48C3A.8 {ECO:0000312|WormBase:Y48C3A.8a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21115607; DOI=10.1242/dev.055350;
RA Zheng Q., Schaefer A.M., Nonet M.L.;
RT "Regulation of C. elegans presynaptic differentiation and neurite branching
RT via a novel signaling pathway initiated by SAM-10.";
RL Development 138:87-96(2011).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Involved cell autonomously in PLM neuron pre-synaptic
CC differentiation by negatively regulating prk-2 expression and in
CC neurite branch positioning. {ECO:0000269|PubMed:21115607}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21115607}. Nucleus
CC {ECO:0000269|PubMed:21115607}. Note=In PLM neurons, translocates into
CC the nucleus during the 3-fold embryonic stage and remains nuclear in
CC the adult. Nuclear localization is idb-1-dependent.
CC {ECO:0000269|PubMed:21115607}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=B7FAS6-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y48C3A.8b};
CC IsoId=B7FAS6-2; Sequence=VSP_057535;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in
CC the head and tail ganglia, the vulva and PLM neurons.
CC {ECO:0000269|PubMed:21115607}.
CC -!- DEVELOPMENTAL STAGE: Expression begins at the 1.5-fold embryonic stage
CC and is retained in the adult. {ECO:0000269|PubMed:21115607}.
CC -!- DISRUPTION PHENOTYPE: Defects in PLM neuron synaptic differentiation
CC characterized by a misalignment of active zones and synaptic vesicles,
CC branch point anterior to the vulva and overextension of synaptic
CC branch. DD motoneurons display similar pre-synaptic defects. Increased
CC expression of prk-2. In addition, mutants display a moderate
CC uncoordinated movement, a small decrease in body size, a defect in egg
CC laying and a slower growth. {ECO:0000269|PubMed:21115607}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL117203; CAV31815.1; -; Genomic_DNA.
DR EMBL; AL117203; CAV31816.1; -; Genomic_DNA.
DR RefSeq; NP_001254379.1; NM_001267450.1. [B7FAS6-1]
DR RefSeq; NP_001254380.1; NM_001267451.1. [B7FAS6-2]
DR AlphaFoldDB; B7FAS6; -.
DR SMR; B7FAS6; -.
DR STRING; 6239.Y48C3A.8a.1; -.
DR EPD; B7FAS6; -.
DR PaxDb; B7FAS6; -.
DR PeptideAtlas; B7FAS6; -.
DR EnsemblMetazoa; Y48C3A.8a.1; Y48C3A.8a.1; WBGene00012990. [B7FAS6-1]
DR EnsemblMetazoa; Y48C3A.8b.1; Y48C3A.8b.1; WBGene00012990. [B7FAS6-2]
DR GeneID; 174975; -.
DR KEGG; cel:CELE_Y48C3A.8; -.
DR CTD; 174975; -.
DR WormBase; Y48C3A.8a; CE43420; WBGene00012990; sam-10. [B7FAS6-1]
DR WormBase; Y48C3A.8b; CE43413; WBGene00012990; sam-10. [B7FAS6-2]
DR eggNOG; KOG4594; Eukaryota.
DR GeneTree; ENSGT00950000183049; -.
DR HOGENOM; CLU_593463_0_0_1; -.
DR InParanoid; B7FAS6; -.
DR OMA; YTIMNPM; -.
DR OrthoDB; 11344at6231; -.
DR PRO; PR:B7FAS6; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00012990; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0048755; P:branching morphogenesis of a nerve; IMP:UniProtKB.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:1902473; P:regulation of protein localization to synapse; IMP:UniProtKB.
DR GO; GO:0007416; P:synapse assembly; IMP:WormBase.
DR InterPro; IPR006594; LisH.
DR SMART; SM00667; LisH; 1.
DR PROSITE; PS50896; LISH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Neurogenesis; Nucleus; Reference proteome.
FT CHAIN 1..455
FT /note="Single-stranded DNA-binding protein homolog sam-10"
FT /id="PRO_0000432619"
FT DOMAIN 19..51
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT REGION 217..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..204
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_057535"
SQ SEQUENCE 455 AA; 46047 MW; D5685FFCA6854D87 CRC64;
MPPQVIQQQQ QSLASEMTAR DRLTSYIYEY LQQTGASKTA ETFKEEVLST NPAAGLAAAN
STKLSDKSFL LEWWLLFWDL YSAAPERRDA GGDPFSAEAK YFHEAMIGMP PGMNGHFAPP
PMGMEMMGGH PGAFGGRFAP GRMPPGAMAP GGMPPGAFPM FPPDPRLQRM APNQGMRMPP
PPVGQPFPGA VGMPRPVGPG APMDMSGMQR FDFMGGPPPG GGAQPFPGAS GSGGMMPNGA
HPHMSLNSPS MGVPPADMPP FMGMPPMPPT SSSAMPFGMS SDHQPMSAGP AAAAPGATTA
GGPGTPGMIG SVPGPGSVPQ VATTSVGSVG TPSSIGQQLH QPKQEITTNG EEIMKTEALT
PTGGGGGGSV PPPPPAATAA VSMNGGGPGS APGSAHSVNN NVNPGTPGSN PLSNPMSNPP
LSSGPPPPGS NDAFGKDDNG EISKIREGLL DGFCA
