SAM35_YEAST
ID SAM35_YEAST Reviewed; 329 AA.
AC P14693; D3DL34;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Sorting assembly machinery 35 kDa subunit;
DE AltName: Full=Mitochondrial 38 kDa outer membrane protein;
DE AltName: Full=TOB complex 38 kDa subunit;
GN Name=SAM35; Synonyms=OMP85, TOB38, TOM38; OrderedLocusNames=YHR083W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-329.
RX PubMed=2558054; DOI=10.1101/gad.3.9.1349;
RA Errede B., Ammerer G.;
RT "STE12, a protein involved in cell-type-specific transcription and signal
RT transduction in yeast, is part of protein-DNA complexes.";
RL Genes Dev. 3:1349-1361(1989).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP IDENTIFICATION IN THE SAM COMPLEX.
RX PubMed=15205677; DOI=10.1038/sj.embor.7400183;
RA Waizenegger T., Habib S.J., Lech M., Mokranjac D., Paschen S.A., Hell K.,
RA Neupert W., Rapaport D.;
RT "Tob38, a novel essential component in the biogenesis of beta-barrel
RT proteins of mitochondria.";
RL EMBO Rep. 5:704-709(2004).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=14699090; DOI=10.1083/jcb.200310092;
RA Gentle I., Gabriel K., Beech P., Waller R., Lithgow T.;
RT "The Omp85 family of proteins is essential for outer membrane biogenesis in
RT mitochondria and bacteria.";
RL J. Cell Biol. 164:19-24(2004).
RN [7]
RP IDENTIFICATION IN THE SAM COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=15326197; DOI=10.1083/jcb.200405138;
RA Ishikawa D., Yamamoto H., Tamura Y., Moritoh K., Endo T.;
RT "Two novel proteins in the mitochondrial outer membrane mediate beta-barrel
RT protein assembly.";
RL J. Cell Biol. 166:621-627(2004).
RN [8]
RP FUNCTION, AND IDENTIFICATION IN THE SAM COMPLEX.
RX PubMed=15067005; DOI=10.1074/jbc.c400120200;
RA Milenkovic D., Kozjak V., Wiedemann N., Lohaus C., Meyer H.E., Guiard B.,
RA Pfanner N., Meisinger C.;
RT "Sam35 of the mitochondrial protein sorting and assembly machinery is a
RT peripheral outer membrane protein essential for cell viability.";
RL J. Biol. Chem. 279:22781-22785(2004).
CC -!- FUNCTION: Essential component of the mitochondrial outer membrane
CC sorting assembly machinery (SAM or TOB) complex, which is required for
CC the sorting of proteins with complicated topology, such as beta-barrel
CC proteins, to the mitochondrial outer membrane after import by the TOM
CC complex. {ECO:0000269|PubMed:14699090, ECO:0000269|PubMed:15067005}.
CC -!- SUBUNIT: Component of the mitochondrial outer membrane sorting assembly
CC machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37
CC and SAM50. {ECO:0000269|PubMed:15067005, ECO:0000269|PubMed:15205677,
CC ECO:0000269|PubMed:15326197}.
CC -!- INTERACTION:
CC P14693; P18409: MDM10; NbExp=5; IntAct=EBI-24602, EBI-10580;
CC P14693; P50110: SAM37; NbExp=7; IntAct=EBI-24602, EBI-2347180;
CC P14693; P53969: SAM50; NbExp=8; IntAct=EBI-24602, EBI-28646;
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:14699090, ECO:0000269|PubMed:15326197}.
CC -!- MISCELLANEOUS: Present with 1470 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U10556; AAB68885.1; -; Genomic_DNA.
DR EMBL; X16112; CAA34245.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06778.1; -; Genomic_DNA.
DR PIR; S46807; S46807.
DR RefSeq; NP_011951.1; NM_001179213.1.
DR PDB; 7BTW; EM; 2.90 A; B=1-329.
DR PDB; 7BTX; EM; 2.80 A; B=1-329.
DR PDB; 7BTY; EM; 3.20 A; B=1-329.
DR PDB; 7E4H; EM; 3.01 A; B=1-329.
DR PDB; 7E4I; EM; 3.05 A; B=1-329.
DR PDBsum; 7BTW; -.
DR PDBsum; 7BTX; -.
DR PDBsum; 7BTY; -.
DR PDBsum; 7E4H; -.
DR PDBsum; 7E4I; -.
DR AlphaFoldDB; P14693; -.
DR SMR; P14693; -.
DR BioGRID; 36518; 96.
DR ComplexPortal; CPX-1744; Mitochondrial sorting and assembly machinery complex.
DR DIP; DIP-1879N; -.
DR IntAct; P14693; 99.
DR MINT; P14693; -.
DR STRING; 4932.YHR083W; -.
DR TCDB; 1.B.33.3.1; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR iPTMnet; P14693; -.
DR MaxQB; P14693; -.
DR PaxDb; P14693; -.
DR PRIDE; P14693; -.
DR EnsemblFungi; YHR083W_mRNA; YHR083W; YHR083W.
DR GeneID; 856483; -.
DR KEGG; sce:YHR083W; -.
DR SGD; S000001125; SAM35.
DR VEuPathDB; FungiDB:YHR083W; -.
DR eggNOG; ENOG502RXPE; Eukaryota.
DR HOGENOM; CLU_073166_0_0_1; -.
DR InParanoid; P14693; -.
DR OMA; FVENECK; -.
DR BioCyc; YEAST:G3O-31130-MON; -.
DR PRO; PR:P14693; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P14693; protein.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IDA:ComplexPortal.
DR GO; GO:0001401; C:SAM complex; IDA:SGD.
DR GO; GO:0070096; P:mitochondrial outer membrane translocase complex assembly; IMP:SGD.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IDA:ComplexPortal.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IMP:SGD.
DR InterPro; IPR021211; SAM35.
DR Pfam; PF10806; SAM35; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..329
FT /note="Sorting assembly machinery 35 kDa subunit"
FT /id="PRO_0000202903"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:7E4I"
FT STRAND 21..23
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 31..40
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 45..50
FT /evidence="ECO:0007829|PDB:7E4H"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:7BTW"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 75..80
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 81..94
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 103..105
FT /evidence="ECO:0007829|PDB:7E4H"
FT STRAND 115..117
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 139..141
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 143..152
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:7BTW"
FT HELIX 158..180
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 181..183
FT /evidence="ECO:0007829|PDB:7BTY"
FT HELIX 185..191
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 199..201
FT /evidence="ECO:0007829|PDB:7BTY"
FT HELIX 204..215
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 217..224
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 227..229
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 230..233
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 235..241
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 242..245
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 248..271
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 272..274
FT /evidence="ECO:0007829|PDB:7BTX"
FT STRAND 279..281
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 284..296
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 305..311
FT /evidence="ECO:0007829|PDB:7BTX"
FT HELIX 314..324
FT /evidence="ECO:0007829|PDB:7BTX"
FT TURN 325..327
FT /evidence="ECO:0007829|PDB:7E4H"
SQ SEQUENCE 329 AA; 37404 MW; 4DCA565B56F35E4D CRC64;
MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG SSELTVEGTY
KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT HSQEQTPSHT CNHEMLVLSR
LSNPDEALPI LVEGYKKRII RSTVAISEIM RSRILDDAEQ LMYYTLLDTV LYDCWITQII
FCASDAQFME LYSCQKLSGS IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS
MQGVYHNHHN SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK
EPIKLKTFVE NECKELVQFA QDTLKNFVQ
