ID   SAM50_BOVIN             Reviewed;         469 AA.
AC   Q2HJ55;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Sorting and assembly machinery component 50 homolog;
GN   Name=SAMM50;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Uterus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a crucial role in the maintenance of the structure of
CC       mitochondrial cristae and the proper assembly of the mitochondrial
CC       respiratory chain complexes. Required for the assembly of TOMM40 into
CC       the TOM complex. {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- SUBUNIT: Associates with the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex, composed of at least MICOS10/MIC10,
CC       CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26
CC       and QIL1/MIC13 (By similarity). This complex was also known under the
CC       names MINOS or MitOS complex (By similarity). The MICOS complex
CC       associates with mitochondrial outer membrane proteins SAMM50, MTX1 and
CC       MTX2 (together described as components of the mitochondrial outer
CC       membrane sorting assembly machinery (SAM) complex) and DNAJC11,
CC       mitochondrial inner membrane protein TMEM11 and with HSPA9 (By
CC       similarity). The MICOS and SAM complexes together with DNAJC11 are part
CC       of a large protein complex spanning both membranes termed the
CC       mitochondrial intermembrane space bridging (MIB) complex (By
CC       similarity). Interacts with IMMT/MIC60. Interacts with CHCHD3/MIC19 (By
CC       similarity). Interacts with ARMC1 (By similarity).
CC       {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC       {ECO:0000250|UniProtKB:Q9Y512}; Multi-pass membrane protein
CC       {ECO:0000250}. Cytoplasm {ECO:0000250|UniProtKB:Q6AXV4}. Mitochondrion
CC       {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- DOMAIN: Its C-terminal part seems to contain many membrane-spanning
CC       sided beta-sheets, that have the potential to adopt a transmembrane
CC       beta-barrel type structure. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SAM50/omp85 family. {ECO:0000305}.
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DR   EMBL; BT025460; ABF57416.1; -; mRNA.
DR   EMBL; BC113305; AAI13306.1; -; mRNA.
DR   RefSeq; NP_001040088.1; NM_001046623.1.
DR   AlphaFoldDB; Q2HJ55; -.
DR   SMR; Q2HJ55; -.
DR   STRING; 9913.ENSBTAP00000055147; -.
DR   PaxDb; Q2HJ55; -.
DR   PRIDE; Q2HJ55; -.
DR   DNASU; 618777; -.
DR   Ensembl; ENSBTAT00000063243; ENSBTAP00000055147; ENSBTAG00000045957.
DR   GeneID; 618777; -.
DR   KEGG; bta:618777; -.
DR   CTD; 25813; -.
DR   VEuPathDB; HostDB:ENSBTAG00000045957; -.
DR   VGNC; VGNC:34278; SAMM50.
DR   eggNOG; KOG2602; Eukaryota.
DR   GeneTree; ENSGT00390000011355; -.
DR   InParanoid; Q2HJ55; -.
DR   OMA; KHPVARF; -.
DR   OrthoDB; 844748at2759; -.
DR   Proteomes; UP000009136; Chromosome 5.
DR   Bgee; ENSBTAG00000045957; Expressed in digestive system secreted substance and 105 other tissues.
DR   ExpressionAtlas; Q2HJ55; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0001401; C:SAM complex; IBA:GO_Central.
DR   GO; GO:0042407; P:cristae formation; ISS:UniProtKB.
DR   GO; GO:0033108; P:mitochondrial respiratory chain complex assembly; ISS:UniProtKB.
DR   GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IBA:GO_Central.
DR   InterPro; IPR000184; Bac_surfAg_D15.
DR   InterPro; IPR039910; D15-like.
DR   InterPro; IPR034746; POTRA.
DR   PANTHER; PTHR12815; PTHR12815; 1.
DR   Pfam; PF01103; Omp85; 1.
DR   PROSITE; PS51779; POTRA; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Membrane; Methylation; Mitochondrion;
KW   Mitochondrion outer membrane; Reference proteome; Transmembrane;
KW   Transmembrane beta strand.
FT   CHAIN           1..469
FT                   /note="Sorting and assembly machinery component 50 homolog"
FT                   /id="PRO_0000383680"
FT   DOMAIN          45..125
FT                   /note="POTRA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT   MOD_RES         255
FT                   /note="N6-methyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y512"
SQ   SEQUENCE   469 AA;  52042 MW;  859F52F5D193A31D CRC64;
     MGTVHARSLE PLPASGPDFG ALGEEAEFVE VEPEAKQEIL ENKDVVVQHV HFDGLGRTKD
     DIIMYEIRDV FKAKNLIEVM RKSHEAREKL LRLGIFRQVD VLIDTCQGDD ALPNGLDVTF
     EVTELRRLTG SYNTMVGNNE GSMVLGLKLP NLLGRAEKVT FQFSYGTKET SYGLSFFKPQ
     PGNFDRNFSV NLYKVTGQFP WSSLRETDRG VSAEYSFPTW KTSHTVKWEG VWRELGCLSR
     VASFAVRKES GHSLKSSLSH SMVIDSRNSS ILPKRGALLK VNQELAGYTG GDVSFLKEDF
     ELQLNKQLIL DTVFSASLWG GMLVPMGDKP SSIADRFYLG GPTSVRGFSM HSVGPQSEGD
     YLGGEAYWAG GLHLYTPLPF RPGQGGFGEL FRTHFFLNAG NLCNLNYGEG PKAHIRKLAE
     CIRWSYGAGI VLRLGNIARL ELNYCVPMGV QRGDRICDGV QFGAGIRFL