ID   SAM50_RAT               Reviewed;         469 AA.
AC   Q6AXV4;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Sorting and assembly machinery component 50 homolog;
GN   Name=Samm50;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=19343716; DOI=10.1002/pmic.200800664;
RA   Maurya D.K., Sundaram C.S., Bhargava P.;
RT   "Proteome profile of the mature rat olfactory bulb.";
RL   Proteomics 9:2593-2599(2009).
CC   -!- FUNCTION: Plays a crucial role in the maintenance of the structure of
CC       mitochondrial cristae and the proper assembly of the mitochondrial
CC       respiratory chain complexes. Required for the assembly of TOMM40 into
CC       the TOM complex. {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- SUBUNIT: Associates with the mitochondrial contact site and cristae
CC       organizing system (MICOS) complex, composed of at least MICOS10/MIC10,
CC       CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26
CC       and QIL1/MIC13 (By similarity). This complex was also known under the
CC       names MINOS or MitOS complex (By similarity). The MICOS complex
CC       associates with mitochondrial outer membrane proteins SAMM50, MTX1 and
CC       MTX2 (together described as components of the mitochondrial outer
CC       membrane sorting assembly machinery (SAM) complex) and DNAJC11,
CC       mitochondrial inner membrane protein TMEM11 and with HSPA9 (By
CC       similarity). The MICOS and SAM complexes together with DNAJC11 are part
CC       of a large protein complex spanning both membranes termed the
CC       mitochondrial intermembrane space bridging (MIB) complex. Interacts
CC       with IMMT/MIC60. Interacts with CHCHD3/MIC19 (By similarity). Interacts
CC       with ARMC1 (By similarity). {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC       {ECO:0000250|UniProtKB:Q9Y512}; Multi-pass membrane protein
CC       {ECO:0000250}. Cytoplasm {ECO:0000269|PubMed:19343716}. Mitochondrion
CC       {ECO:0000250|UniProtKB:Q9Y512}.
CC   -!- DOMAIN: Its C-terminal part seems to contain many membrane-spanning
CC       sided beta-sheets, that have the potential to adopt a transmembrane
CC       beta-barrel type structure. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SAM50/omp85 family. {ECO:0000305}.
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DR   EMBL; BC079302; AAH79302.1; -; mRNA.
DR   RefSeq; NP_001004241.1; NM_001004241.1.
DR   AlphaFoldDB; Q6AXV4; -.
DR   SMR; Q6AXV4; -.
DR   BioGRID; 256455; 2.
DR   STRING; 10116.ENSRNOP00000016520; -.
DR   iPTMnet; Q6AXV4; -.
DR   PhosphoSitePlus; Q6AXV4; -.
DR   jPOST; Q6AXV4; -.
DR   PaxDb; Q6AXV4; -.
DR   PRIDE; Q6AXV4; -.
DR   DNASU; 300111; -.
DR   Ensembl; ENSRNOT00000016520; ENSRNOP00000016520; ENSRNOG00000011952.
DR   GeneID; 300111; -.
DR   KEGG; rno:300111; -.
DR   CTD; 25813; -.
DR   RGD; 1303219; Samm50.
DR   eggNOG; KOG2602; Eukaryota.
DR   GeneTree; ENSGT00390000011355; -.
DR   HOGENOM; CLU_014798_3_0_1; -.
DR   InParanoid; Q6AXV4; -.
DR   OMA; KHPVARF; -.
DR   OrthoDB; 844748at2759; -.
DR   PhylomeDB; Q6AXV4; -.
DR   TreeFam; TF106126; -.
DR   Reactome; R-RNO-9013404; RAC2 GTPase cycle.
DR   PRO; PR:Q6AXV4; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000011952; Expressed in heart and 19 other tissues.
DR   Genevisible; Q6AXV4; RN.
DR   GO; GO:0016021; C:integral component of membrane; ISO:RGD.
DR   GO; GO:0005741; C:mitochondrial outer membrane; ISO:RGD.
DR   GO; GO:0005739; C:mitochondrion; ISO:RGD.
DR   GO; GO:0001401; C:SAM complex; ISO:RGD.
DR   GO; GO:0042407; P:cristae formation; ISS:UniProtKB.
DR   GO; GO:0033108; P:mitochondrial respiratory chain complex assembly; ISS:UniProtKB.
DR   GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; ISO:RGD.
DR   InterPro; IPR000184; Bac_surfAg_D15.
DR   InterPro; IPR039910; D15-like.
DR   InterPro; IPR034746; POTRA.
DR   PANTHER; PTHR12815; PTHR12815; 1.
DR   Pfam; PF01103; Omp85; 1.
DR   PROSITE; PS51779; POTRA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Membrane; Methylation; Mitochondrion;
KW   Mitochondrion outer membrane; Reference proteome; Transmembrane;
KW   Transmembrane beta strand.
FT   CHAIN           1..469
FT                   /note="Sorting and assembly machinery component 50 homolog"
FT                   /id="PRO_0000286400"
FT   DOMAIN          45..125
FT                   /note="POTRA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT   MOD_RES         255
FT                   /note="N6-methyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y512"
SQ   SEQUENCE   469 AA;  51960 MW;  600F6CC96DA5297F CRC64;
     MGTVHARSLE PLPSSGTDFG ALGEEAEFVE VEPEAKQEIL ENKDVVVQHV HFDGLERTKD
     DIIICEIGEV FKAKNLIEVM RRSHEAREKL LRLGIFRQVD VLIDTCHGED ALPNGLDVTF
     EVTELRRLTG SYNTMVGNNE GSMVLGLKLP NLLGRAEKVT FQFSYGTKET SYGLSFFKPQ
     PGNFEKNFSV NLYKVTGQFP WSSLRETDRG VSAEYSFPLC KTSHTVKWEG VWRELGCLAR
     TASFAVRKES GHSLKSSLSH AMVIDSRNSS ILPRRGALLK VNQELAGYTG GDVSFIKEDF
     ELQLNKPLVL DSVFSTSLWG GMLVPIGDKL SSIADRFYLG GPTSVRGFSM HSIGPQSEGD
     YLGGEAYWAG GLHLYTPLPF RPGQGGFGEL FRTHFFLNAG NLCNLNYGEG PRAHIRKLAE
     CIRWSYGAGI VLRLGNIARL ELNYCIPMGV QRGDRICDGV QFGAGIRFL