ID   SAMC2_ARATH             Reviewed;         345 AA.
AC   F4HT41; Q500W9; Q9FX15;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Probable S-adenosylmethionine carrier 2, chloroplastic;
DE   Flags: Precursor;
GN   Name=SAMC2; OrderedLocusNames=At1g34065; ORFNames=F12G12.11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-345.
RA   Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT   "Arabidopsis cDNA clones.";
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 25-345, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=16950860; DOI=10.1104/pp.106.086975;
RA   Palmieri L., Arrigoni R., Blanco E., Carrari F., Zanor M.I.,
RA   Studart-Guimareas C., Fernie A.R., Palmieri F.;
RT   "Molecular identification of an Arabidopsis S-adenosylmethionine
RT   transporter. Analysis of organ distribution, bacterial expression,
RT   reconstitution into liposomes, and functional characterization.";
RL   Plant Physiol. 142:855-865(2006).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=17098813; DOI=10.1105/tpc.105.040741;
RA   Bouvier F., Linka N., Isner J.C., Mutterer J., Weber A.P.M., Camara B.;
RT   "Arabidopsis SAMT1 defines a plastid transporter regulating plastid
RT   biogenesis and plant development.";
RL   Plant Cell 18:3088-3105(2006).
CC   -!- FUNCTION: Probable S-adenosylmethionine (SAM) transporter able to
CC       catalyze both uniport and exchange reactions through membranes.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed at low levels in seedlings, leaves,
CC       flowers, stems and roots. {ECO:0000269|PubMed:16950860}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG12530.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAY34159.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC015446; AAG12530.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE31668.1; -; Genomic_DNA.
DR   EMBL; BT022098; AAY34159.1; ALT_INIT; mRNA.
DR   EMBL; AM260491; CAJ91124.1; -; mRNA.
DR   PIR; F86464; F86464.
DR   RefSeq; NP_564436.4; NM_103129.5.
DR   AlphaFoldDB; F4HT41; -.
DR   SMR; F4HT41; -.
DR   STRING; 3702.AT1G34065.1; -.
DR   PaxDb; F4HT41; -.
DR   PRIDE; F4HT41; -.
DR   ProteomicsDB; 232840; -.
DR   EnsemblPlants; AT1G34065.1; AT1G34065.1; AT1G34065.
DR   GeneID; 840304; -.
DR   Gramene; AT1G34065.1; AT1G34065.1; AT1G34065.
DR   KEGG; ath:AT1G34065; -.
DR   Araport; AT1G34065; -.
DR   TAIR; locus:505006167; AT1G34065.
DR   eggNOG; KOG0768; Eukaryota.
DR   HOGENOM; CLU_015166_3_0_1; -.
DR   InParanoid; F4HT41; -.
DR   OrthoDB; 1538959at2759; -.
DR   PRO; PR:F4HT41; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4HT41; baseline and differential.
DR   Genevisible; F4HT41; AT.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0000095; F:S-adenosyl-L-methionine transmembrane transporter activity; IBA:GO_Central.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Chloroplast; Membrane; Plastid; Reference proteome; Repeat;
KW   S-adenosyl-L-methionine; Transit peptide; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..31
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..345
FT                   /note="Probable S-adenosylmethionine carrier 2,
FT                   chloroplastic"
FT                   /id="PRO_0000424772"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          76..148
FT                   /note="Solcar 1"
FT   REPEAT          157..239
FT                   /note="Solcar 2"
FT   REPEAT          252..334
FT                   /note="Solcar 3"
SQ   SEQUENCE   345 AA;  36883 MW;  3F259E58838F0F14 CRC64;
     MTKALSGFCC SLSLSTLVRS SSSHMDSDIV SSSIDRSQTA MPDALAFKSI NDPIKNQINS
     CAAICVKQDD PCHFLRVLYE SLITGGLAGV VVEAALYPID TIKTRIQVAR DGGKIIWKGL
     YSGLGGNLVG VLPASALFFG VYEPTKQKLL KVLPDNLSAV AHLAAGALGG AVSSIVRVPT
     EVVKQRMQTG QFVSAPDAVR LIIAKEGFGG MYAGYGSFLL RDLPFDALQF CVYEQLRIGY
     KLAARRDLND PENAMIGAFA GAVTGVLTTP LDVIKTRLMV QGSGTQYKGV SDCIKTIIRE
     EGSSALWKGM GPRVLWIGIG GSIFFGVLEK TKQILSERSQ KSHNA