SAMC_DROME
ID SAMC_DROME Reviewed; 283 AA.
AC Q9VBN7; Q4V4S1;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=S-adenosylmethionine mitochondrial carrier protein homolog;
GN ORFNames=CG4743;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=27313316; DOI=10.1101/gad.282277.116;
RA Parkhitko A.A., Binari R., Zhang N., Asara J.M., Demontis F., Perrimon N.;
RT "Tissue-specific down-regulation of S-adenosyl-homocysteine via suppression
RT of dAhcyL1/dAhcyL2 extends health span and life span in Drosophila.";
RL Genes Dev. 30:1409-1422(2016).
CC -!- FUNCTION: Mitochondrial solute carriers shuttle metabolites,
CC nucleotides, and cofactors through the mitochondrial inner membrane.
CC May mediate the transport of S-adenosylmethionine (SAM) into the
CC mitochondria (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VBN7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VBN7-2; Sequence=VSP_031065;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in extended
CC lifespan. {ECO:0000269|PubMed:27313316}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF56493.1; -; Genomic_DNA.
DR EMBL; BT022260; AAY54676.1; -; mRNA.
DR EMBL; BT022935; AAY55351.1; -; mRNA.
DR RefSeq; NP_651415.1; NM_143158.2. [Q9VBN7-2]
DR AlphaFoldDB; Q9VBN7; -.
DR SMR; Q9VBN7; -.
DR BioGRID; 68012; 1.
DR IntAct; Q9VBN7; 5.
DR STRING; 7227.FBpp0084307; -.
DR PaxDb; Q9VBN7; -.
DR DNASU; 43101; -.
DR EnsemblMetazoa; FBtr0084933; FBpp0084307; FBgn0039357. [Q9VBN7-2]
DR GeneID; 43101; -.
DR KEGG; dme:Dmel_CG4743; -.
DR UCSC; CG4743-RA; d. melanogaster. [Q9VBN7-1]
DR FlyBase; FBgn0039357; CG4743.
DR VEuPathDB; VectorBase:FBgn0039357; -.
DR eggNOG; KOG0768; Eukaryota.
DR GeneTree; ENSGT00550000074950; -.
DR HOGENOM; CLU_015166_3_0_1; -.
DR InParanoid; Q9VBN7; -.
DR OMA; PRVGWIS; -.
DR PhylomeDB; Q9VBN7; -.
DR Reactome; R-DME-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR BioGRID-ORCS; 43101; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43101; -.
DR PRO; PR:Q9VBN7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039357; Expressed in thoracico-abdominal ganglion (Drosophila) and 31 other tissues.
DR Genevisible; Q9VBN7; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0000095; F:S-adenosyl-L-methionine transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0015805; P:S-adenosyl-L-methionine transport; ISS:UniProtKB.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 4.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat;
KW S-adenosyl-L-methionine; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..283
FT /note="S-adenosylmethionine mitochondrial carrier protein
FT homolog"
FT /id="PRO_0000317592"
FT TRANSMEM 14..34
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 11..84
FT /note="Solcar 1"
FT REPEAT 93..178
FT /note="Solcar 2"
FT REPEAT 187..275
FT /note="Solcar 3"
FT VAR_SEQ 1..4
FT /note="MHLL -> MAAELGLESAAGSVAIKM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_031065"
SQ SEQUENCE 283 AA; 31038 MW; BCCC9149F02D6C65 CRC64;
MHLLQEPVNK LKFFHALVAG GVAGMVVDIA LFPIDTVKTR LQSELGFWRA GGFRGIYKGL
APAAAGSAPT AALFFCTYEC GKQFLSSVTQ TKDSPYVHMA AASAAEVLAC LIRVPVEIAK
QRSQTLQGNK QSGLQILLRA YRTEGLKRGL YRGFGSTIMR EIPFSLIQFP LWEYFKLQWT
PLTGFDSTPF SVALCGAVAG GISAGLTTPL DVVKTRIMLA ERESLNRRRS ARRILHGIYL
ERGFSGLFAG FVPRVLWITL GGAFFFGFYD LTTRILGATS TDH
