SAMD5_MOUSE
ID SAMD5_MOUSE Reviewed; 173 AA.
AC Q3V1H9; A6H5X3; Q8BJC5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Sterile alpha motif domain-containing protein 5;
DE Short=SAM domain-containing protein 5;
GN Name=Samd5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye, and Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=28388653; DOI=10.1371/journal.pone.0175355;
RA Yagai T., Matsui S., Harada K., Inagaki F.F., Saijou E., Miura Y.,
RA Nakanuma Y., Miyajima A., Tanaka M.;
RT "Expression and localization of sterile alpha motif domain containing 5 is
RT associated with cell type and malignancy of biliary tree.";
RL PLoS ONE 12:E0175355-E0175355(2017).
RN [4] {ECO:0007744|PDB:5ZRZ}
RP X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) OF 1-66 IN COMPLEX WITH EPHA5,
RP SUBUNIT, AND MUTAGENESIS OF ASP-24; TYR-27 AND ASP-28.
RX PubMed=29749928; DOI=10.7554/elife.35677;
RA Wang Y., Shang Y., Li J., Chen W., Li G., Wan J., Liu W., Zhang M.;
RT "Specific Eph receptor-cytoplasmic effector signaling mediated by SAM-SAM
RT domain interactions.";
RL Elife 7:0-0(2018).
CC -!- SUBUNIT: Interacts promiscuously (via SAM domain) with EPHA5, EPHA6,
CC EPHA7, EPHA8, EPHB1, EPHB2, EPHB3 and EPHB4 (via SAM domain) (in
CC vitro). {ECO:0000269|PubMed:29749928}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:28388653}.
CC -!- TISSUE SPECIFICITY: Detected in kidney glomeruli, and in peribiliary
CC gland (PBG) cells at the hepatic hilum (at protein level). Detected in
CC liver, kidney and small intestine. {ECO:0000269|PubMed:28388653}.
CC -!- INDUCTION: Strongly induced in EPCAM-positive liver cells in response
CC to liver damage caused by long-term 3,5-diethoxycarbonyl-1,4-dihydro-
CC collidine (DDC) feeding. {ECO:0000269|PubMed:28388653}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC39899.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK087498; BAC39899.1; ALT_FRAME; mRNA.
DR EMBL; AK132444; BAE21172.1; -; mRNA.
DR EMBL; BC145672; AAI45673.1; -; mRNA.
DR EMBL; BC145674; AAI45675.1; -; mRNA.
DR CCDS; CCDS35836.1; -.
DR RefSeq; NP_796245.2; NM_177271.3.
DR PDB; 5ZRZ; X-ray; 1.89 A; B=1-66.
DR PDBsum; 5ZRZ; -.
DR AlphaFoldDB; Q3V1H9; -.
DR SMR; Q3V1H9; -.
DR STRING; 10090.ENSMUSP00000097648; -.
DR PhosphoSitePlus; Q3V1H9; -.
DR PaxDb; Q3V1H9; -.
DR PRIDE; Q3V1H9; -.
DR ProteomicsDB; 253395; -.
DR DNASU; 320825; -.
DR GeneID; 320825; -.
DR KEGG; mmu:320825; -.
DR UCSC; uc007eiw.1; mouse.
DR CTD; 389432; -.
DR MGI; MGI:2444815; Samd5.
DR eggNOG; KOG4384; Eukaryota.
DR InParanoid; Q3V1H9; -.
DR OrthoDB; 1498045at2759; -.
DR PhylomeDB; Q3V1H9; -.
DR TreeFam; TF339222; -.
DR BioGRID-ORCS; 320825; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Samd5; mouse.
DR PRO; PR:Q3V1H9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q3V1H9; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR Pfam; PF00536; SAM_1; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome.
FT CHAIN 1..173
FT /note="Sterile alpha motif domain-containing protein 5"
FT /id="PRO_0000311362"
FT DOMAIN 1..65
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT REGION 89..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 24
FT /note="D->A: Strongly reduced binding affinity for EPHA5
FT SAM domain."
FT /evidence="ECO:0000269|PubMed:29749928"
FT MUTAGEN 27
FT /note="Y->F: Abolishes binding to EPHA5 SAM domain."
FT /evidence="ECO:0000269|PubMed:29749928"
FT MUTAGEN 28
FT /note="D->A: Strongly reduced binding affinity for EPHA5
FT SAM domain."
FT /evidence="ECO:0000269|PubMed:29749928"
FT CONFLICT 11
FT /note="R -> K (in Ref. 2; AAI45673/AAI45675)"
FT /evidence="ECO:0000305"
FT HELIX 5..12
FT /evidence="ECO:0007829|PDB:5ZRZ"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:5ZRZ"
FT HELIX 19..24
FT /evidence="ECO:0007829|PDB:5ZRZ"
FT HELIX 30..33
FT /evidence="ECO:0007829|PDB:5ZRZ"
FT HELIX 38..44
FT /evidence="ECO:0007829|PDB:5ZRZ"
FT HELIX 49..62
FT /evidence="ECO:0007829|PDB:5ZRZ"
SQ SEQUENCE 173 AA; 19221 MW; 0888866977989924 CRC64;
MCTNIVYEWL RALQLPQYAE SFVDNGYDDL EVCKQIGDPD LDAIGVLAPA HRRRILEAVH
RLREQDAAAA GLYFTLEPQP VPPAPLVEAV PPGRRGEPCG SSAQGTRGDP RGQPGAPCSR
ELVSYPKLKL KIMIRDKLVR DGIHLSKPPY SRKVPMAGIL EYLMNWPKSS QNH
