ID   SAMHT_CHLT2             Reviewed;         327 AA.
AC   B0B8F4;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=S-adenosylmethionine/S-adenosylhomocysteine transporter;
DE            Short=SAM/SAH transporter;
DE            Short=SAMHT;
GN   OrderedLocusNames=CTL0843;
OS   Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=471472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=434/Bu / ATCC VR-902B;
RX   PubMed=18032721; DOI=10.1101/gr.7020108;
RA   Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P.,
RA   Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D.,
RA   Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N.;
RT   "Chlamydia trachomatis: genome sequence analysis of lymphogranuloma
RT   venereum isolates.";
RL   Genome Res. 18:161-171(2008).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=434/Bu / ATCC VR-902B;
RX   PubMed=21558433; DOI=10.1128/mbio.00051-11;
RA   Binet R., Fernandez R.E., Fisher D.J., Maurelli A.T.;
RT   "Identification and characterization of the Chlamydia trachomatis L2 S-
RT   adenosylmethionine transporter.";
RL   MBio 2:E51-E51(2011).
CC   -!- FUNCTION: Transports S-adenosylmethionine (SAM) and S-
CC       adenosylhomocysteine (SAH). Allows bacteria to acquire SAM from the
CC       eukaryotic host cell and to likely remove the toxic by-product SAH.
CC       {ECO:0000269|PubMed:21558433}.
CC   -!- ACTIVITY REGULATION: CCCP treatment reduces SAM intracellular uptake by
CC       50%. {ECO:0000269|PubMed:21558433}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=5.9 uM for SAM {ECO:0000269|PubMed:21558433};
CC         Vmax=32 pmol/min/mg enzyme {ECO:0000269|PubMed:21558433};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
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DR   EMBL; AM884176; CAP04280.1; -; Genomic_DNA.
DR   RefSeq; WP_009873914.1; NC_010287.1.
DR   RefSeq; YP_001654913.1; NC_010287.1.
DR   AlphaFoldDB; B0B8F4; -.
DR   SMR; B0B8F4; -.
DR   TCDB; 2.A.7.3.20; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; CAP04280; CAP04280; CTL0843.
DR   KEGG; ctb:CTL0843; -.
DR   PATRIC; fig|471472.4.peg.904; -.
DR   HOGENOM; CLU_080359_0_0_0; -.
DR   OMA; AYGLFFY; -.
DR   Proteomes; UP000000795; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Repeat; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..327
FT                   /note="S-adenosylmethionine/S-adenosylhomocysteine
FT                   transporter"
FT                   /id="PRO_5000300993"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          34..157
FT                   /note="EamA 1"
FT   DOMAIN          189..313
FT                   /note="EamA 2"
SQ   SEQUENCE   327 AA;  36069 MW;  07E295402C94E407 CRC64;
     MVAVKALLFA CTLRTCVFKP CCDMAIFLIF LNAFIWSSSF ALSKSAMEAA APLFVTGSRM
     VLAGVVLFGL LLCKRESLRL PRPAIMPIVL LSVIGFYLTN VLEFIGLQRL SSSTACFIYG
     FSPFTAAFCS YVQLREVVTW KKLGGLSLGL VSYLVYLLFG GSEDVAEWGW QLGLPELLLI
     AATCLSSYGW TLLRKLGRRC ESLSMTAINA YAMVIAGVLS LIHSAVTEVW NPVPVENPLL
     FLQAIGALVI FSNLICYNLF AKLLRSFSST FLSFCNLVMP LFASFFGWLL LGESFPPGLL
     FAVGFMVLGC RLIYHEEFRQ GYVLTSE