ID   SAM_RICFE               Reviewed;         294 AA.
AC   Q4UNE6;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=S-adenosylmethionine uptake transporter;
GN   Name=sam; OrderedLocusNames=RF_0061;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- FUNCTION: Transports S-adenosylmethionine. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
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DR   EMBL; CP000053; AAY60912.1; -; Genomic_DNA.
DR   RefSeq; WP_011270416.1; NC_007109.1.
DR   AlphaFoldDB; Q4UNE6; -.
DR   SMR; Q4UNE6; -.
DR   STRING; 315456.RF_0061; -.
DR   EnsemblBacteria; AAY60912; AAY60912; RF_0061.
DR   KEGG; rfe:RF_0061; -.
DR   eggNOG; COG0697; Bacteria.
DR   HOGENOM; CLU_032828_0_0_5; -.
DR   OMA; PFFWEVP; -.
DR   OrthoDB; 1420919at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane; Repeat;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..294
FT                   /note="S-adenosylmethionine uptake transporter"
FT                   /id="PRO_0000280996"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          21..141
FT                   /note="EamA 1"
FT   DOMAIN          160..280
FT                   /note="EamA 2"
SQ   SEQUENCE   294 AA;  33016 MW;  54F089B6CCD64760 CRC64;
     MNDALKTYLT GIGWFLLSLV SSSANDVMSK YLGTRLHSFE VAFFRFFFSS IVLLPFVFYH
     GKNTLKTSRP FVHILRGLLL FFGMTSWTYG LTIAPVTTAT VVSFSIPLFT LILAVFFLNE
     NIIWQRWVVT VVGFIGLVVT LKPHTEDFNP EILYFVLAAI SFAMLDIINK KFVIKESMIS
     MLFYSAIVTA VVSLPVASQY WLTPSGFELA LLFVLGSSGS LILFFLLKAF SMVDATATAP
     YRYLELVISA IAAYFIFSEF PDKSTLHGAV IIIPATLFII YSEKKAMSRK HESQ