ID   SAM_RICPR               Reviewed;         294 AA.
AC   Q9ZE70;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=S-adenosylmethionine uptake transporter;
GN   Name=sam; OrderedLocusNames=RP076;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND ACTIVITY REGULATION.
RC   STRAIN=Madrid E;
RX   PubMed=12730161; DOI=10.1128/jb.185.10.3031-3035.2003;
RA   Tucker A.M., Winkler H.H., Driskell L.O., Wood D.O.;
RT   "S-adenosylmethionine transport in Rickettsia prowazekii.";
RL   J. Bacteriol. 185:3031-3035(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- FUNCTION: Transports S-adenosylmethionine.
CC       {ECO:0000269|PubMed:12730161}.
CC   -!- ACTIVITY REGULATION: Transport is inhibited by S-adenosylethionine and
CC       to a lesser extent by S-adenosylhomocysteine. Unlike eukaryotic
CC       transporters is not inhibited by sinfungin. Also inhibited by 2.4-
CC       dinitrophenol, suggesting transport is an energy-dependent process.
CC       {ECO:0000269|PubMed:12730161}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
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DR   EMBL; AJ235270; CAA14547.1; -; Genomic_DNA.
DR   PIR; D71716; D71716.
DR   RefSeq; NP_220470.1; NC_000963.1.
DR   RefSeq; WP_004596540.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZE70; -.
DR   SMR; Q9ZE70; -.
DR   STRING; 272947.RP076; -.
DR   TCDB; 2.A.7.3.7; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; CAA14547; CAA14547; CAA14547.
DR   GeneID; 57569204; -.
DR   KEGG; rpr:RP076; -.
DR   PATRIC; fig|272947.5.peg.77; -.
DR   eggNOG; COG0697; Bacteria.
DR   HOGENOM; CLU_032828_0_0_5; -.
DR   OMA; PFFWEVP; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..294
FT                   /note="S-adenosylmethionine uptake transporter"
FT                   /id="PRO_0000280997"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          22..141
FT                   /note="EamA 1"
FT   DOMAIN          160..280
FT                   /note="EamA 2"
SQ   SEQUENCE   294 AA;  33092 MW;  3DE6527258466AB1 CRC64;
     MNDALKTYLN GICWFLLSLV TSSINDVMSK YLGTRLHSFE VAFFRFFFSS IVLLPFVVYY
     GKNALKTSRP FVHVLRGLLL FFGMTSWTYG LTIAPVTTAT VVSFAIPLFT LILAVFILNE
     NIIWQRWVVT VVGFIGLVVM LKPHTKDFNP EILYLILAAI SFAMLDIINK KFVVKESMLS
     MLFYSAIVTA MVSLPVAMQY WITPSSFELA LLFVLGSSGS FILFFLLKAF SIVDATATAP
     YRYLELVISA IAAYFIFNEF PDKSTVHGAV IIIPATLFII YSEKKSMSSK HESQ