ID   SANA_ECOLI              Reviewed;         239 AA.
AC   P0AFY2; P33017; P76438; Q2MAT5;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Protein SanA;
GN   Name=sanA; Synonyms=yeiF; OrderedLocusNames=b2144, JW2132;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8550448; DOI=10.1128/jb.178.1.94-102.1996;
RA   Rida S., Caillet J., Alix J.-H.;
RT   "Amplification of a novel gene, sanA, abolishes a vancomycin-sensitive
RT   defect in Escherichia coli.";
RL   J. Bacteriol. 178:94-102(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-152.
RC   STRAIN=K12 / BHB2600;
RA   Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA   Church G.M.;
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RX   PubMed=1567863; DOI=10.1021/bi00132a003;
RA   Yang C., Carlow D., Wolfenden R., Short S.A.;
RT   "Cloning and nucleotide sequence of the Escherichia coli cytidine deaminase
RT   (ccd) gene.";
RL   Biochemistry 31:4168-4174(1992).
CC   -!- FUNCTION: Participates in the barrier function of the cell envelope.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Single-pass membrane
CC       protein.
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DR   EMBL; Z47804; CAA87766.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75205.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76621.1; -; Genomic_DNA.
DR   EMBL; U00007; AAA60509.1; -; Genomic_DNA.
DR   EMBL; M60916; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; G64982; G64982.
DR   RefSeq; NP_416649.1; NC_000913.3.
DR   RefSeq; WP_000920064.1; NZ_STEB01000002.1.
DR   AlphaFoldDB; P0AFY2; -.
DR   BioGRID; 4259167; 7.
DR   IntAct; P0AFY2; 2.
DR   STRING; 511145.b2144; -.
DR   TCDB; 9.B.114.1.1; the vancomycin-sensitivity protein (sana) family.
DR   jPOST; P0AFY2; -.
DR   PaxDb; P0AFY2; -.
DR   PRIDE; P0AFY2; -.
DR   EnsemblBacteria; AAC75205; AAC75205; b2144.
DR   EnsemblBacteria; BAE76621; BAE76621; BAE76621.
DR   GeneID; 66673960; -.
DR   GeneID; 949003; -.
DR   KEGG; ecj:JW2132; -.
DR   KEGG; eco:b2144; -.
DR   PATRIC; fig|1411691.4.peg.98; -.
DR   EchoBASE; EB1960; -.
DR   eggNOG; COG2949; Bacteria.
DR   HOGENOM; CLU_051474_0_2_6; -.
DR   InParanoid; P0AFY2; -.
DR   OMA; YDEPDAM; -.
DR   PhylomeDB; P0AFY2; -.
DR   BioCyc; EcoCyc:EG12025-MON; -.
DR   PRO; PR:P0AFY2; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; IMP:EcoCyc.
DR   CDD; cd06259; YdcF-like; 1.
DR   InterPro; IPR003848; DUF218.
DR   InterPro; IPR023604; Uncharacterised_SanA.
DR   Pfam; PF02698; DUF218; 1.
DR   PIRSF; PIRSF005011; SanA; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..239
FT                   /note="Protein SanA"
FT                   /id="PRO_0000097576"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        7..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        24..239
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        156..239
FT                   /note="IALHMGIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLV
FT                   PIPAMHQVPEDAQGYPAVTPEQLLELQKKQGK -> MRCIWDSGSVLCRTVTERYAVST
FT                   YS (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   239 AA;  27287 MW;  574EF9B02249547C CRC64;
     MLKRVFLSLL VLIGLLLLTV LGLDRWMSWK TAPYIYDELQ DLPYRQVGVV LGTAKYYRTG
     VINQYYRYRI QGAINAYNSG KVNYLLLSGD NALQSYNEPM TMRKDLIAAG VDPSDIVLDY
     AGFRTLDSIV RTRKVFDTND FIIITQRFHC ERALFIALHM GIQAQCYAVP SPKDMLSVRI
     REFAARFGAL ADLYIFKREP RFLGPLVPIP AMHQVPEDAQ GYPAVTPEQL LELQKKQGK