SAO1_CAEEL
ID SAO1_CAEEL Reviewed; 245 AA.
AC C6KRN1; C6KRN2; Q1ZXT3; Q1ZXT4;
DT 16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Suppressor of aph-1 {ECO:0000312|WormBase:R10D12.14c};
GN Name=sao-1 {ECO:0000312|WormBase:R10D12.14c};
GN ORFNames=R10D12.14 {ECO:0000312|WormBase:R10D12.14c};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LEU-59.
RX PubMed=22209900; DOI=10.1534/genetics.111.136804;
RA Hale V.A., Guiney E.L., Goldberg L.Y., Haduong J.H., Kwartler C.S.,
RA Scangos K.W., Goutte C.;
RT "Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that
RT interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.";
RL Genetics 190:1043-1057(2012).
CC -!- FUNCTION: Involved in negative regulation of early and late embryonic
CC Notch signaling. {ECO:0000269|PubMed:22209900}.
CC -!- INTERACTION:
CC C6KRN1; Q22799: dlc-1; NbExp=4; IntAct=EBI-323135, EBI-328324;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=c {ECO:0000312|WormBase:R10D12.14c};
CC IsoId=C6KRN1-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:R10D12.14a};
CC IsoId=C6KRN1-2; Sequence=VSP_057839;
CC Name=b {ECO:0000312|WormBase:R10D12.14b};
CC IsoId=C6KRN1-3; Sequence=VSP_057840;
CC Name=d {ECO:0000312|WormBase:R10D12.14d};
CC IsoId=C6KRN1-4; Sequence=VSP_057840, VSP_057841;
CC -!- DISRUPTION PHENOTYPE: Partially penetrant phenotypes when temperature
CC elevated to 25 degrees Celsius which include embryonic lethality,
CC reduced brood size, egg retention and an increased incidence of males.
CC {ECO:0000269|PubMed:22209900}.
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DR EMBL; Z81109; CAJ85777.1; -; Genomic_DNA.
DR EMBL; Z81109; CAJ85778.1; -; Genomic_DNA.
DR EMBL; Z81109; CAZ65518.1; -; Genomic_DNA.
DR EMBL; Z81109; CAZ65519.1; -; Genomic_DNA.
DR RefSeq; NP_001041150.1; NM_001047685.1.
DR RefSeq; NP_001041151.1; NM_001047686.2. [C6KRN1-3]
DR RefSeq; NP_001256550.1; NM_001269621.1. [C6KRN1-1]
DR RefSeq; NP_001256551.1; NM_001269622.1. [C6KRN1-4]
DR AlphaFoldDB; C6KRN1; -.
DR SMR; C6KRN1; -.
DR IntAct; C6KRN1; 3.
DR MINT; C6KRN1; -.
DR STRING; 6239.R10D12.14c; -.
DR EPD; C6KRN1; -.
DR PaxDb; C6KRN1; -.
DR PeptideAtlas; C6KRN1; -.
DR EnsemblMetazoa; R10D12.14a.1; R10D12.14a.1; WBGene00011195. [C6KRN1-2]
DR EnsemblMetazoa; R10D12.14b.1; R10D12.14b.1; WBGene00011195. [C6KRN1-3]
DR EnsemblMetazoa; R10D12.14c.1; R10D12.14c.1; WBGene00011195. [C6KRN1-1]
DR EnsemblMetazoa; R10D12.14d.1; R10D12.14d.1; WBGene00011195. [C6KRN1-4]
DR GeneID; 3565237; -.
DR UCSC; R10D12.14b; c. elegans.
DR CTD; 3565237; -.
DR WormBase; R10D12.14a; CE40107; WBGene00011195; sao-1. [C6KRN1-2]
DR WormBase; R10D12.14b; CE40108; WBGene00011195; sao-1. [C6KRN1-3]
DR WormBase; R10D12.14c; CE12698; WBGene00011195; sao-1. [C6KRN1-1]
DR WormBase; R10D12.14d; CE43865; WBGene00011195; sao-1. [C6KRN1-4]
DR eggNOG; KOG1862; Eukaryota.
DR HOGENOM; CLU_078058_0_0_1; -.
DR InParanoid; C6KRN1; -.
DR OMA; WFYQGED; -.
DR OrthoDB; 1451346at2759; -.
DR SignaLink; C6KRN1; -.
DR PRO; PR:C6KRN1; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00011195; Expressed in adult organism and 3 other tissues.
DR ExpressionAtlas; C6KRN1; baseline and differential.
DR GO; GO:0005938; C:cell cortex; IDA:WormBase.
DR GO; GO:0032154; C:cleavage furrow; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0071987; F:WD40-repeat domain binding; IPI:WormBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IGI:WormBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0060625; P:regulation of protein deneddylation; IGI:WormBase.
DR CDD; cd00072; GYF; 1.
DR Gene3D; 3.30.1490.40; -; 1.
DR InterPro; IPR003169; GYF.
DR InterPro; IPR035445; GYF-like_dom_sf.
DR Pfam; PF02213; GYF; 1.
DR SMART; SM00444; GYF; 1.
DR SUPFAM; SSF55277; SSF55277; 1.
DR PROSITE; PS50829; GYF; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Notch signaling pathway;
KW Reference proteome.
FT CHAIN 1..245
FT /note="Suppressor of aph-1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000433780"
FT DOMAIN 12..60
FT /note="GYF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00101"
FT REGION 126..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..166
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 160..180
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_057839"
FT VAR_SEQ 160..180
FT /note="TRLNEQTLQQPPSWLIALGLA -> FQ (in isoform b and
FT isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057840"
FT VAR_SEQ 224
FT /note="E -> EFQ (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_057841"
FT MUTAGEN 59
FT /note="L->F: In ik1; temperature-sensitive mutant with low
FT incidence of embryonic lethality."
FT /evidence="ECO:0000269|PubMed:22209900"
SQ SEQUENCE 245 AA; 27532 MW; A817206830A9CB66 CRC64;
MHKNGNNGPV IDTKWHYLGP DSEKYGPYMS KDMLFWLQAG YFNDGLQLKT ENEPNYHTLG
EWSQLLGTHP FSMPVHSLDA TIAQMNSMRP HGAMMMVPPG LQNQFQPPMP MRFPPFLPMP
LLHQMNQNGP PMGAQMHSQP PSEPIDAGSL SHTPDSENET RLNEQTLQQP PSWLIALGLA
GHGRKPHHHQ QILAHQHIPQ MQHANVATDQ VVMKSVECQT EPVEISKEQA SRVLSELLGQ
MVIIN
