SAP1_ARATH
ID SAP1_ARATH Reviewed; 168 AA.
AC Q6NNI8; Q9LNA2;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Zinc finger A20 and AN1 domain-containing stress-associated protein 1;
DE Short=AtSAP1;
GN Name=SAP1; OrderedLocusNames=At1g12440; ORFNames=F5O11.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=17033811; DOI=10.1007/s00438-006-0165-1;
RA Vij S., Tyagi A.K.;
RT "Genome-wide analysis of the stress associated protein (SAP) gene family
RT containing A20/AN1 zinc-finger(s) in rice and their phylogenetic
RT relationship with Arabidopsis.";
RL Mol. Genet. Genomics 276:565-575(2006).
RN [5]
RP STRUCTURE BY NMR OF 84-149.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the ZF-AN1 domain from Arabidopsis thaliana F5O11.17
RT protein.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- FUNCTION: May be involved in environmental stress response.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79653.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC025416; AAF79653.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28882.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28883.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58832.1; -; Genomic_DNA.
DR EMBL; BT010824; AAR24191.1; -; mRNA.
DR EMBL; BT011299; AAR92335.1; -; mRNA.
DR RefSeq; NP_001321241.1; NM_001332036.1.
DR RefSeq; NP_172706.1; NM_101116.6.
DR RefSeq; NP_849652.1; NM_179321.3.
DR PDB; 1WFP; NMR; -; A=89-149.
DR PDBsum; 1WFP; -.
DR AlphaFoldDB; Q6NNI8; -.
DR SMR; Q6NNI8; -.
DR BioGRID; 23041; 8.
DR IntAct; Q6NNI8; 7.
DR STRING; 3702.AT1G12440.2; -.
DR PaxDb; Q6NNI8; -.
DR PRIDE; Q6NNI8; -.
DR ProteomicsDB; 226665; -.
DR EnsemblPlants; AT1G12440.1; AT1G12440.1; AT1G12440.
DR EnsemblPlants; AT1G12440.2; AT1G12440.2; AT1G12440.
DR EnsemblPlants; AT1G12440.3; AT1G12440.3; AT1G12440.
DR GeneID; 837801; -.
DR Gramene; AT1G12440.1; AT1G12440.1; AT1G12440.
DR Gramene; AT1G12440.2; AT1G12440.2; AT1G12440.
DR Gramene; AT1G12440.3; AT1G12440.3; AT1G12440.
DR KEGG; ath:AT1G12440; -.
DR Araport; AT1G12440; -.
DR TAIR; locus:2034650; AT1G12440.
DR eggNOG; KOG3173; Eukaryota.
DR HOGENOM; CLU_057016_5_3_1; -.
DR InParanoid; Q6NNI8; -.
DR OMA; PETHECE; -.
DR OrthoDB; 1551371at2759; -.
DR PhylomeDB; Q6NNI8; -.
DR EvolutionaryTrace; Q6NNI8; -.
DR PRO; PR:Q6NNI8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NNI8; baseline and differential.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 4.10.1110.10; -; 1.
DR InterPro; IPR035896; AN1-like_Znf.
DR InterPro; IPR002653; Znf_A20.
DR InterPro; IPR000058; Znf_AN1.
DR Pfam; PF01754; zf-A20; 1.
DR Pfam; PF01428; zf-AN1; 1.
DR SMART; SM00259; ZnF_A20; 1.
DR SMART; SM00154; ZnF_AN1; 1.
DR SUPFAM; SSF118310; SSF118310; 1.
DR PROSITE; PS51036; ZF_A20; 1.
DR PROSITE; PS51039; ZF_AN1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..168
FT /note="Zinc finger A20 and AN1 domain-containing stress-
FT associated protein 1"
FT /id="PRO_0000066577"
FT ZN_FING 13..47
FT /note="A20-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00451"
FT ZN_FING 103..149
FT /note="AN1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT REGION 49..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..93
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00451"
FT BINDING 23
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00451"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00451"
FT BINDING 38
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00451"
FT BINDING 109
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 123
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 125
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 130
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 133
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 139
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT BINDING 141
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00449"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:1WFP"
FT TURN 117..119
FT /evidence="ECO:0007829|PDB:1WFP"
FT TURN 131..133
FT /evidence="ECO:0007829|PDB:1WFP"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:1WFP"
SQ SEQUENCE 168 AA; 17820 MW; 5C33C5A98C14E672 CRC64;
MGSEQNDSTS FSPSEPKLCV KGCGFFGSPS NMNLCSKCYR DIRATEEQTA SAKAAVEKSL
NPNKPKTQPQ QSQEITQGVL GSGSSSSSTR GGDSAAAPLD PPKSTATRCL SCNKKVGVTG
FKCRCGSTFC GTHRYPESHE CQFDFKGVAR EAIAKANPVV KADKVDRI
