SAP47_DROME
ID SAP47_DROME Reviewed; 551 AA.
AC Q960T2; A4V2Z5; Q24502; Q24503; Q8INC9; Q9VF07;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Synapse-associated protein of 47 kDa;
GN Name=Sap47; ORFNames=CG8884;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS F AND I), AND TISSUE SPECIFICITY.
RC STRAIN=Berlin; TISSUE=Head;
RX PubMed=7494462; DOI=10.1016/0169-328x(95)00058-z;
RA Reichmuth C., Becker S., Benz M., Debel K., Reisch D., Heimbeck G.,
RA Hofbauer A., Klagges B., Pflugfelder G.O., Buchner E.;
RT "The sap47 gene of Drosophila melanogaster codes for a novel conserved
RT neuronal protein associated with synaptic terminals.";
RL Brain Res. Mol. Brain Res. 32:45-54(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-182; THR-186;
RP SER-433 AND THR-530, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=A; Synonyms=C, D, E, H;
CC IsoId=Q960T2-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q960T2-2; Sequence=VSP_007393, VSP_007394;
CC Name=F; Synonyms=1;
CC IsoId=Q960T2-4; Sequence=VSP_007389, VSP_007390, VSP_007393,
CC VSP_007394;
CC Name=G;
CC IsoId=Q960T2-5; Sequence=VSP_007389;
CC Name=I; Synonyms=2;
CC IsoId=Q960T2-3; Sequence=VSP_007389, VSP_007390, VSP_007391,
CC VSP_007392;
CC -!- TISSUE SPECIFICITY: Expressed specifically in neurons and transported
CC to synaptic terminals. {ECO:0000269|PubMed:7494462}.
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DR EMBL; X80110; CAA56415.1; -; mRNA.
DR EMBL; X80111; CAA56416.1; -; mRNA.
DR EMBL; AE014297; AAF55255.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13682.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13683.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13684.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13685.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13686.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13687.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13688.1; -; Genomic_DNA.
DR EMBL; AY051871; AAK93295.1; -; mRNA.
DR PIR; S60653; S60653.
DR RefSeq; NP_001287350.1; NM_001300421.1. [Q960T2-3]
DR RefSeq; NP_524371.1; NM_079647.4. [Q960T2-4]
DR RefSeq; NP_732088.1; NM_169684.3. [Q960T2-1]
DR RefSeq; NP_732089.1; NM_169685.3. [Q960T2-1]
DR RefSeq; NP_732090.1; NM_169686.3. [Q960T2-1]
DR RefSeq; NP_732091.1; NM_169687.3. [Q960T2-1]
DR RefSeq; NP_732092.2; NM_169688.3. [Q960T2-3]
DR RefSeq; NP_732093.1; NM_169689.3. [Q960T2-5]
DR RefSeq; NP_732094.1; NM_169690.3. [Q960T2-2]
DR AlphaFoldDB; Q960T2; -.
DR SMR; Q960T2; -.
DR BioGRID; 66992; 14.
DR DIP; DIP-18495N; -.
DR IntAct; Q960T2; 3.
DR STRING; 7227.FBpp0082658; -.
DR iPTMnet; Q960T2; -.
DR PaxDb; Q960T2; -.
DR PRIDE; Q960T2; -.
DR DNASU; 41938; -.
DR EnsemblMetazoa; FBtr0083204; FBpp0082658; FBgn0013334. [Q960T2-1]
DR EnsemblMetazoa; FBtr0083205; FBpp0082659; FBgn0013334. [Q960T2-2]
DR EnsemblMetazoa; FBtr0083206; FBpp0082660; FBgn0013334. [Q960T2-1]
DR EnsemblMetazoa; FBtr0083207; FBpp0082661; FBgn0013334. [Q960T2-1]
DR EnsemblMetazoa; FBtr0083208; FBpp0082662; FBgn0013334. [Q960T2-1]
DR EnsemblMetazoa; FBtr0083209; FBpp0082663; FBgn0013334. [Q960T2-4]
DR EnsemblMetazoa; FBtr0083210; FBpp0082664; FBgn0013334. [Q960T2-5]
DR EnsemblMetazoa; FBtr0301655; FBpp0290869; FBgn0013334. [Q960T2-3]
DR EnsemblMetazoa; FBtr0344384; FBpp0310757; FBgn0013334. [Q960T2-3]
DR GeneID; 41938; -.
DR KEGG; dme:Dmel_CG8884; -.
DR CTD; 41938; -.
DR FlyBase; FBgn0013334; Sap47.
DR VEuPathDB; VectorBase:FBgn0013334; -.
DR eggNOG; KOG4310; Eukaryota.
DR GeneTree; ENSGT00390000007662; -.
DR InParanoid; Q960T2; -.
DR OMA; FQTTCES; -.
DR PhylomeDB; Q960T2; -.
DR BioGRID-ORCS; 41938; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Sap47; fly.
DR GenomeRNAi; 41938; -.
DR PRO; PR:Q960T2; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0013334; Expressed in brain and 29 other tissues.
DR ExpressionAtlas; Q960T2; baseline and differential.
DR Genevisible; Q960T2; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IDA:UniProtKB.
DR GO; GO:0008306; P:associative learning; IMP:FlyBase.
DR GO; GO:0007268; P:chemical synaptic transmission; NAS:UniProtKB.
DR GO; GO:0048172; P:regulation of short-term neuronal synaptic plasticity; IDA:FlyBase.
DR GO; GO:0038203; P:TORC2 signaling; IBA:GO_Central.
DR Gene3D; 1.10.3970.10; -; 1.
DR InterPro; IPR005607; BSD_dom.
DR InterPro; IPR035925; BSD_dom_sf.
DR Pfam; PF03909; BSD; 1.
DR SMART; SM00751; BSD; 1.
DR SUPFAM; SSF140383; SSF140383; 1.
DR PROSITE; PS50858; BSD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..551
FT /note="Synapse-associated protein of 47 kDa"
FT /id="PRO_0000097584"
FT DOMAIN 295..347
FT /note="BSD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT REGION 20..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 117..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 370..385
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 178
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 182
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 186
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 433
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 530
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 72..88
FT /note="RLPKSASLVDSLVSEAT -> S (in isoform F, isoform G and
FT isoform I)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007389"
FT VAR_SEQ 197..204
FT /note="GKGDEVKI -> V (in isoform F and isoform I)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007390"
FT VAR_SEQ 369..370
FT /note="AN -> ED (in isoform I)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007391"
FT VAR_SEQ 370..374
FT /note="NEVAT -> LAFNY (in isoform B and isoform F)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007393"
FT VAR_SEQ 371..551
FT /note="Missing (in isoform I)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007392"
FT VAR_SEQ 375..551
FT /note="Missing (in isoform B and isoform F)"
FT /evidence="ECO:0000303|PubMed:7494462"
FT /id="VSP_007394"
SQ SEQUENCE 551 AA; 56980 MW; 430392D0EAD5916E CRC64;
MFSGLTNQFT SLVGAVKGGA GDEDVPAPTG DAPAAAPAAS TSVEATASSA VDPEAAAAAG
GEGLEGEEAG KRLPKSASLV DSLVSEATGW LGSAKGWLGN ASIPSMPAMP SMPSMPAMPA
MPSIPSIPGL RKGAGADGAE GAEGAVAGEG GAAASGAVSG GEDDDKSRYI SATEGADSHP
ASGGGTPTGD EGQIGQGKGD EVKITTKVTQ QAKHFGSFLS SAISKAGSKI KETVKDNTIL
DSFNKEQEAF IKGQGGVGNG AAPWIGHANE AKIKEEILGL SQDRRNFVRA PPAGVDFEFS
YDTAYPTAIA IMAEDKALET MRFELVPKII TEENFWRNYF YRVSLIIQAA ELGTLGADGV
GQASSGEDAN EVATKEKKSK TAEPAKGDSS VKAIAEQPKA VIEPEAQECD VQAAKSKAKA
KAQAGKELGQ KISESEFVSD DFQASSESDL AEIQDGMRKL GIDSMTQQAL AATDEEQWEK
DLEAELKDYE VVDEGGTGGD GGGGRRKGRK AGEDDTEADE DEPTISNLRT RSTNNDWEEY
ADLIEDTDDL K
