ID   SAPA_ECOLI              Reviewed;         547 AA.
AC   Q47622; P77358;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Probable ABC transporter periplasmic-binding protein SapA {ECO:0000305};
DE   Flags: Precursor;
GN   Name=sapA; OrderedLocusNames=b1294, JW1287;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / FRAG5;
RX   PubMed=11700350; DOI=10.1099/00221287-147-11-2991;
RA   Harms C., Domoto Y., Celik C., Rahe E., Stumpe S., Schmid R., Nakamura T.,
RA   Bakker E.P.;
RT   "Identification of the ABC protein SapD as the subunit that confers ATP
RT   dependence to the K+-uptake systems Trk(H) and Trk(G) from Escherichia coli
RT   K-12.";
RL   Microbiology 147:2991-3003(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   34-35.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / BW25113, and K12 / MG1655 / ATCC 47076;
RX   PubMed=27803167; DOI=10.1074/jbc.m116.762450;
RA   Sugiyama Y., Nakamura A., Matsumoto M., Kanbe A., Sakanaka M., Higashi K.,
RA   Igarashi K., Katayama T., Suzuki H., Kurihara S.;
RT   "A novel putrescine exporter SapBCDF of Escherichia coli.";
RL   J. Biol. Chem. 291:26343-26351(2016).
CC   -!- FUNCTION: Not part of a putrescine export system (PubMed:27803167).
CC       Very similar to a S.typhimurium protein implicated in antimicrobial
CC       peptide resistance, but the SapBCDF operon in E.coli is implicated in
CC       putrescine export (PubMed:27803167). {ECO:0000269|PubMed:27803167}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: No change in extracellular putrescine levels.
CC       {ECO:0000269|PubMed:27803167}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC       {ECO:0000305}.
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DR   EMBL; X97282; CAA65937.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74376.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA14855.2; -; Genomic_DNA.
DR   PIR; A64878; A64878.
DR   RefSeq; NP_415810.1; NC_000913.3.
DR   RefSeq; WP_001250216.1; NZ_SSZK01000012.1.
DR   AlphaFoldDB; Q47622; -.
DR   SMR; Q47622; -.
DR   BioGRID; 4261257; 254.
DR   ComplexPortal; CPX-4422; Putative peptide ABC transporter.
DR   DIP; DIP-10822N; -.
DR   IntAct; Q47622; 5.
DR   STRING; 511145.b1294; -.
DR   TCDB; 3.A.1.5.42; the atp-binding cassette (abc) superfamily.
DR   jPOST; Q47622; -.
DR   PaxDb; Q47622; -.
DR   PRIDE; Q47622; -.
DR   EnsemblBacteria; AAC74376; AAC74376; b1294.
DR   EnsemblBacteria; BAA14855; BAA14855; BAA14855.
DR   GeneID; 945873; -.
DR   KEGG; ecj:JW1287; -.
DR   KEGG; eco:b1294; -.
DR   PATRIC; fig|1411691.4.peg.985; -.
DR   EchoBASE; EB4155; -.
DR   eggNOG; COG4166; Bacteria.
DR   HOGENOM; CLU_017028_7_0_6; -.
DR   InParanoid; Q47622; -.
DR   OMA; NQYVRLV; -.
DR   PhylomeDB; Q47622; -.
DR   BioCyc; EcoCyc:SAPA-MON; -.
DR   BRENDA; 7.6.2.11; 2026.
DR   PRO; PR:Q47622; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR   GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR   GO; GO:1904680; F:peptide transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015833; P:peptide transport; IBA:GO_Central.
DR   InterPro; IPR030678; Peptide/Ni-bd.
DR   InterPro; IPR039424; SBP_5.
DR   InterPro; IPR023765; SBP_5_CS.
DR   InterPro; IPR000914; SBP_5_dom.
DR   PANTHER; PTHR30290; PTHR30290; 1.
DR   Pfam; PF00496; SBP_bac_5; 1.
DR   PIRSF; PIRSF002741; MppA; 1.
DR   PROSITE; PS01040; SBP_BACTERIAL_5; 1.
PE   3: Inferred from homology;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..547
FT                   /note="Probable ABC transporter periplasmic-binding protein
FT                   SapA"
FT                   /id="PRO_0000031801"
FT   CONFLICT        34..35
FT                   /note="SG -> RV (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   547 AA;  61565 MW;  EB552BB3B8E102BF CRC64;
     MRQVLSSLLV IAGLVSGQAI AAPESPPHAD IRDSGFVYCV SGQVNTFNPS KASSGLIVDT
     LAAQFYDRLL DVDPYTYRLM PELAESWEVL DNGATYRFHL RRDVPFQKTD WFTPTRKMNA
     DDVVFTFQRI FDRNNPWHNV NGSNFPYFDS LQFADNVKSV RKLDNHTVEF RLAQPDASFL
     WHLATHYASV MSAEYARKLE KEDRQEQLDR QPVGTGPYQL SEYRAGQFIR LQRHDDFWRG
     KPLMPQVVVD LGSGGTGRLS KLLTGECDVL AWPAASQLSI LRDDPRLRLT LRPGMNVAYL
     AFNTAKPPLN NPAVRHALAL AINNQRLMQS IYYGTAETAA SILPRASWAY DNEAKITEYN
     PAKSREQLKS LGLENLTLKL WVPTRSQAWN PSPLKTAELI QADMAQVGVK VVIVPVEGRF
     QEARLMDMSH DLTLSGWATD SNDPDSFFRP LLSCAAIHSQ TNLAHWCDPK FDSVLRKALS
     SQQLAARIEA YDEAQSILAQ ELPILPLASS LRLQAYRYDI KGLVLSPFGN ASFAGVYREK
     QDEVKKP