ID   SAP_ARATH               Reviewed;         446 AA.
AC   Q9FKH1; Q9ZRQ7;
DT   09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Transcriptional regulator STERILE APETALA;
GN   Name=SAP; OrderedLocusNames=At5g35770; ORFNames=MXH1.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC   STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX   PubMed=10215627; DOI=10.1101/gad.13.8.1002;
RA   Byzova M.V., Franken J., Aarts M.G.M., de Almeida-Engler J., Engler G.,
RA   Mariani C., van Lookeren Campagne M.M., Angenent G.C.;
RT   "Arabidopsis STERILE APETALA, a multifunctional gene regulating
RT   inflorescence, flower, and ovule development.";
RL   Genes Dev. 13:1002-1014(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Transcriptional regulator involved in the specification of
CC       floral identity. Acts as A class cadastral protein by repressing the C
CC       class floral homeotic gene AGAMOUS in the external flower organs in
CC       association with APETALA2 and other repressors. Is required to maintain
CC       floral meristem identity in concert with AGAMOUS. Interacts also with
CC       APETALA2 to ensure the normal development of ovule.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in inflorescence and floral meristems,
CC       young floral organ primordia, and later in ovule primordia.
CC   -!- MISCELLANEOUS: Mutations in the SAP gene result in the ectopic
CC       expression of AGAMOUS, leading to partial homeotic transformation of
CC       the external floral organs. Mutations affect equally ovule development
CC       and cause floral sterility.
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DR   EMBL; AJ223125; CAA11128.1; -; mRNA.
DR   EMBL; AB011485; BAB09276.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94017.1; -; Genomic_DNA.
DR   PIR; T51368; T51368.
DR   RefSeq; NP_198426.1; NM_122968.2.
DR   AlphaFoldDB; Q9FKH1; -.
DR   BioGRID; 18809; 15.
DR   IntAct; Q9FKH1; 8.
DR   STRING; 3702.AT5G35770.1; -.
DR   PaxDb; Q9FKH1; -.
DR   PRIDE; Q9FKH1; -.
DR   ProteomicsDB; 226646; -.
DR   EnsemblPlants; AT5G35770.1; AT5G35770.1; AT5G35770.
DR   GeneID; 833556; -.
DR   Gramene; AT5G35770.1; AT5G35770.1; AT5G35770.
DR   KEGG; ath:AT5G35770; -.
DR   Araport; AT5G35770; -.
DR   TAIR; locus:2177271; AT5G35770.
DR   eggNOG; ENOG502QPKU; Eukaryota.
DR   HOGENOM; CLU_614456_0_0_1; -.
DR   InParanoid; Q9FKH1; -.
DR   OMA; WRIMVID; -.
DR   OrthoDB; 574224at2759; -.
DR   PhylomeDB; Q9FKH1; -.
DR   PRO; PR:Q9FKH1; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKH1; baseline and differential.
DR   Genevisible; Q9FKH1; AT.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:TAIR.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IMP:TAIR.
DR   GO; GO:0009554; P:megasporogenesis; IMP:TAIR.
DR   GO; GO:0046622; P:positive regulation of organ growth; IMP:TAIR.
DR   GO; GO:0030163; P:protein catabolic process; IMP:TAIR.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   SUPFAM; SSF81383; SSF81383; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Flowering; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..446
FT                   /note="Transcriptional regulator STERILE APETALA"
FT                   /id="PRO_0000097586"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        75
FT                   /note="H -> L (in Ref. 1; CAA11128)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        153
FT                   /note="A -> G (in Ref. 1; CAA11128)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        328
FT                   /note="Q -> P (in Ref. 1; CAA11128)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   446 AA;  49306 MW;  D099C0F2DED475F9 CRC64;
     MSTSSSSSDN GAGGSGGVFE APSPSRPRRG ANDVWPEPFL ESLAVQVAVN ASTSAGLLAA
     APALANVFRV CTTWHAVSRS DHLWQLLSRQ VWARTHLMHD TWRDEFIYRH RTARNFRTRT
     HTYFTLQFDP SDVDEPDSLS CRCLTLSDLY LAAGFADGTV RLFLLNNRLH VRTLRPPLRD
     RFGRFSRAVS GIVISDSRLT FATMDGDIHV AEIDGVGHTR TAYAGDIVND GALVDFTGCG
     RWWVGLFAGV PGRAFHIWDC NSEETTFVGG TLTDPEAVMG WHTLTELTTS LGRLRISGNE
     TAVACTRWRI MVIDLRNQGV IIGEDEEQRR GLIVTGFDAN DEAYVRLDSR GNASVRRVNT
     QQTVCEFRVS GAAQRRVMGC VNRLHALMCA GGIMRVWEVE RGEYLYSIRE RVGEVDAIVA
     DDRHVAVASA SSTAQSIIHL WDFGAL